Photosynthetica, 2011 (vol. 49), issue 3

Photosynthetica 2011, 49(3):353 | DOI: 10.1007/s11099-011-0044-9

Biphasic regulation of superoxide radical levels in Mn-depleted and photoactivated photosystem II

Y. G. Song1,2, B. Liu3, Y. P. Liu2, L. B. Du1, F. A. Villamena2, Y. Liu1,*
1 State Key laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, China
2 Center for Biomedical EPR Spectroscopy and Imaging, The Davis Heart and Lung Research Institute, the Division of Cardiovascular Medicine, Department of Internal Medicine, and Department of Pharmacology, College of Medicine, The Ohio State University, Columbus, USA
3 Cardiovascular Research Center, Shantou University Medical College, Shantou, China

In the past decades, it has become clear that superoxide radical (O2 .-) can be generated from photosystem II (PSII) during photosynthesis. Depending on the extent of its accumulation, O2 .- plays an important role in plant physiology and pathology. The photoinhibition/repair cycle is a typical process in PSII which is mainly responsible for the survival of plants under the photoinihibition condition. It is therefore of significant importance to determine O2 .- production in this cycle, and then explore how O2 .- is controlled by PSII within a normal physiological level. With this in mind, we herein investigate the variation of the O2 .- levels in PSII under Mn-depleted and photoactivated conditions mimicking the photoinhibition/repair cycle in vitro. The effect of intrinsic SOD-like component on the O2 .- levels was also studied. Results show that PSII has the ability to regulate the O2 .- levels in these two processes by simultaneously modulating the O2 .- generation activity and intrinsic SOD-like activity. This finding could shed new lights on the photoprotective property of PSII against O2 .- and other reactive oxygen species.

Keywords: biphasic regulation; cytochrome b559; Mn-depletion; photoactivation; photosystem II; superoxide radical

Received: November 15, 2010; Accepted: May 11, 2011; Published: September 1, 2011Show citation

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Song, Y.G., Liu, B., Liu, Y.P., Du, L.B., Villamena, F.A., & Liu, Y. (2011). Biphasic regulation of superoxide radical levels in Mn-depleted and photoactivated photosystem II. Photosynthetica, 49(3), 353. doi: 10.1007/s11099-011-0044-9.
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