Photosynthetica, 2011 (vol. 49), issue 3

Photosynthetica 2011, 49(3):371 | DOI: 10.1007/s11099-011-0040-0

Black leaf-clips increased minimum fluorescence emission in clipped leaves exposed to high solar radiation during dark adaptation

P. Giorio1,*
1 Istituto per i Sistemi Agricoli e Forestali del Mediterraneo, Consiglio Nazionale delle Ricerche (CNR-I.S.A.FO.M.), Ercolano (NA), Italy

Tomato and pepper leaves were clipped with black leaf clips for dark adaptation under solar radiation in the late spring or early summer 2010 in southern Italy. The leaves showed highly variable maximum PSII quantum yield (Fv/Fm = 0.026-0.802) using a continuous-excitation fluorometer Pocket PEA. These results were confirmed using the modulated fluorometer FMS1 on tomato leaves in mid summer, with Fv/Fm as low as 0.222 ± 0.277 due to nearly equal minimum (Fo) and maximum (Fm) fluorescence emission. A significant clip effect on Fv/Fm occurred after only 12 (tomato) or 25 (pepper) min. Increasing the leaf temperature from 25 to 50°C reportedly induced an Fo increase and Fm decrease so that Fv/Fm approached zero. The hypothesis that black leaf clips overheated under intense solar irradiance was verified by shrouding the clipped leaves with aluminum foil. In clipped leaves of pepper, Fv/Fm with the black clip/Pocket-PEA was 0.769 ± 0.025 (shrouded) and as low as 0.271 ± 0.163 (nonshrouded), the latter showing a double Fo and 32% lower Fm. An 8% clip effect on Fv/Fm was observed with the white clip/FMS1. To avoid the clip effect in high irradiance environments, Fv/Fm measurements with black clip/Pocket PEA system required leaf dark adaptation with radiation-reflecting shrouds. It would be useful if manufacturing companies could develop better radiation-reflecting leaf clips for the Pocket PEA fluorometer.

Keywords: leaf-clip effect; pepper; photochemical efficiency; radiation; temperature; tomato

Received: September 13, 2010; Accepted: June 22, 2011; Published: September 1, 2011Show citation

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Giorio, P. (2011). Black leaf-clips increased minimum fluorescence emission in clipped leaves exposed to high solar radiation during dark adaptation. Photosynthetica, 49(3), 371. doi: 10.1007/s11099-011-0040-0.
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    References

    1. Baker, N.R.: Chlorophyll fluorescence: a probe of photosynthesis in vivo. - Annu. Rev. Plant Biol. 59: 89-113, 2008. Go to original source...
    2. Bilger, H.W., Schreiber, U., Lange, O.L.: Determination of leaf heat resistance: comparative investigation of chlorophyll fluorescence changes and tissue necrosis methods.- Oecologia 63: 256-262, 1984. Go to original source...
    3. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    4. Hansatech Instruments: Hansatech Fluorescence Monitoring System User Manual. - England, King's Lynn, Norfolk 1997.
    5. Hansatech Instruments: Operating Instructions for Plant Efficiency Analyser Advanced Fluorescence Analysis, Summary Version. - England, King's Lynn, Norfolk 1999.
    6. Hansatech Instruments: Operations Manual, Setup, Installation & Maintenance: Handy PEA, Pocket PEA & PEA Plus Software, version 1.0. - England, King's Lynn, Norfolk 2006.
    7. Ilík, P., Nauš, J., Cikánek, D., Novotný, R.: Chlorophyll fluorescence changes at high temperatures induced by linear heating of greening barley leaves. - Photosynth. Res. 44: 271-275, 1995. Go to original source...
    8. Kitajima, M., Butler, W.L.: Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. - Acta Biochim. Biophys. 376: 105-115, 1975. Go to original source...
    9. Kouřil, R., Lazár, D., Ilík, P., Skotnica, J., Krchňák, P., Nauš, J.: High-temperature induced chlorophyll fluorescence rise in plants at 40-50 °C: experimental and theoretical approach.- Photosynth. Res. 81: 49-66, 2004. Go to original source...
    10. Kuropatwa, R., Nauš, J., Mašláň, M., Dvořák, L.: Basic properties of the chlorophyll fluorescence temperature curve in barley leaves. - Photosynthetica 27: 129-138, 1992.
    11. Lazár, D.: Chlorophyll a fluorescence induction. - Biochim. Biophys. Acta 1412: 1-28, 1999. Go to original source...
    12. Lazár, D.: The polyphasic chloropyll a fluorescence rise measured under high intensity of exciting light. - Funct. Plant Biol. 33: 9-30, 2006. Go to original source...
    13. Lazár, D, Nauš, J.: Statistical properties of chlorophyll fluorescence induction parameters. - Photosynthetica 35: 121-127, 1998. Go to original source...
    14. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence - a practical guide. - J. Exp. Bot. 51: 659-668, 2000. Go to original source...
    15. Nauš, J., Kuropatwa, R., Klinkovský T., Ilík, P., Lattová J., Pavlová, Z.: Heat injury of barley leaves detected by the chlorophyll fluorescence temperature curve. - Biochim. Biophys. Acta 1101: 359-362, 1992. Go to original source...
    16. Ögren, E., Baker, N.R.: Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light. - Plant, Cell Environ. 8: 539-547, 1985.
    17. Pospíšil, P., Skotnica, J., Nauš, J.: Low and high temperature of minimum F 0 and maximum F M chlorophyll fluorescence in vivo. - Biochim Biophys Acta 163: 95-99, 1998.
    18. Roháček, K., Barták, M.: Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. - Photosynthetica 37: 339-363, 1999.
    19. Roháček, K.: Method for resolution and quantification of componenets of the non-photochemical quenching (qN).- Photosynth. Res. 105: 101-113, 2010. Go to original source...
    20. Schreiber U.: Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer.- Photosynth. Res. 9: 261-272, 1986. Go to original source...
    21. Schreiber, U., Bilger, W., Schliwa, U.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. - Photosynth. Res. 10: 51-62, 1986. Go to original source...
    22. Strasser, R.J., Srivastava, A, Tsimilli-Michael, M.: The fluorescence transient as a tool to characterize and screen photosynthetic samples. - In: Yunus, M., Pathre, U., Mohanty, P. (ed.): Probing Photosynthesis: Mechanism, Regulation and Adaptation. Pp. 443-480. Taylor and Francis, London 2000.
    23. Strasser, R.J., Tsimilli-Michael, M., Srivastava, A.: Analysis of the fluorescence transient. - In: Papageorgiou, G.C., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Pp. 321-362. Springer, Dordrecht 2004. Go to original source...
    24. Sušila, P., Lazár, D., Ilík, P., Tomek, P., Nauš, J.: The gradient exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise.- Photosynthetica 42: 161-172, 2004.
    25. van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. - Photosynth. Res. 27: 121-133, 1990. Go to original source...
    26. Weng, J.-H.: Underestimate of photosystem 2 efficiency in the field due to high leaf temperature resulting from leaf clipping and its amendment. - Photosynthetica 44: 467-470, 2006. Go to original source...
    27. Weng, J.-H., Lai, M.-F.: Estimating heat tolerance among plant species by two chlorophyll fluorescence parameters.- Photosynthetica 43: 439-444, 2005. Go to original source...
    28. Yamane, Y., Kashino, Y., Koike, H., Satoh, K.: Increase in the fluorescence Fo level and reversible inhibition of Photosystem II reaction center by high-temperature treatments in higher plants. - Photosynth. Res. 52: 57-64, 1997. Go to original source...

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