Contacts

Group head: 

Tomáš Moravec

Centre: 

Lysolaje

Address: 

Rozvojová 263, 165 02 Praha 6 - Lysolaje

Email: 

moravecueb [dot] cas [dot] cz

Phone: 

+420 225 106 822

www: 

Laboratory of Virology

Research areas of the laboratory:
Understanding and management of plant viral diseases
·         Study of molecular properties of plant viruses by immunological and molecular biological methods
·         Genome mapping of plant viruses, viral isolates and phylogenetic analysis
·         Preparation of polyclonal antibodies against recombinant viral structural and nonstructural proteins for investigation of virus life-cycle
·         Virus detection using antibodies- and/or DNA/RNA-based methods (LAMP, RT-LAMP, PCR, RT-PCR, qPCR)
Biotechnological utilization of plant viruses
·         Design of plant viral vectors based on several RNA and DNA plant viruses
·         Adaptation of plant viral vectors to GoldenBraid cloning system
·         Transient expression of heterologous proteins in plants using vectors derived from plant viruses
·         Peptide/epitope display on the surface of virus particles (electron microscopy)
Metabolic engineering of plant cells
·         Development of platform for expression and accumulation of authentic human glycoproteins such as antibodies in plants
·         Plant genome editing (Crispr, RNAi)
·         Protein expression using plant tissue cultures
Influence of abiotic stress on plant virus infection
Analysis of virus infection upon heat, drought and cold treatment of plants