Photosynthetica, 2011 (vol. 49), issue 3

Photosynthetica 2011, 49(3):389 | DOI: 10.1007/s11099-011-0047-6

Responses of gas exchange, cellular membrane integrity, and antioxidant enzymes activities of salinity-stressed winter wheat to ozone pollution

Y. H. Zheng1,2,*, Y. G. Li1, W. R. Xia4, H. Xu1, B. Y. Su3, G. M. Jiang1,3, T. Y. Ning3,*
1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing, China
2 State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Water and Soil Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi, P. R. China
3 State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, China
4 Dezhou University, Dezhou, China

A sand-culture experiment was conducted in open-top chambers which were constructed in a greenhouse to investigate the responses of salt-stressed wheat (Triticum aestivum L.) to O3. Plant seeding of JN17 (a popular winter wheat cultivar) was grown in saltless (-S) and saline (+S, 100 mM NaCl) conditions combined with charcoal-filtered air (CF, < 5 ppb O3) and elevated O3 (+O3, 80 ± 5 ppb, 8 h day-1) for 30 d. O3 significantly reduced net photosynthetic rate (PN), stomatal conductance, chlorophyll contents and plant biomass in -S treatment, but no considerable differences were noted in those parameters between +O3+S and CF+S treatments. O3-induced loss in cellular membrane integrity was significant in -S plants, but not in +S plants evidenced by significant elevations being measured in electrolyte leakage (EL) and malondialdehyde (MDA) content in -S plants, but not in +S plants. Both O3 and salinity increased proline content and stimulated antioxidant enzymes activities. Soluble protein increased by salinity but decreased by O3. Abscisic acid (ABA) was significantly elevated by O3 in -S plants but not in +S plants. The results of this study suggested that the specificity of different agricultural environments should be considered in order to develop reliable prediction models on O3 damage to wheat plants.

Keywords: abscisic acid; antioxidants; gas exchange; ozone; salinity; Triticum aestivum L.

Received: November 13, 2010; Accepted: May 10, 2011; Published: September 1, 2011Show citation

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Zheng, Y.H., Li, Y.G., Xia, W.R., Xu, H., Su, B.Y., Jiang, G.M., & Ning, T.Y. (2011). Responses of gas exchange, cellular membrane integrity, and antioxidant enzymes activities of salinity-stressed winter wheat to ozone pollution. Photosynthetica, 49(3), 389. doi: 10.1007/s11099-011-0047-6.
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