Photosynthetica, 2011 (vol. 49), issue 1

Photosynthetica 2011, 49(1):48-54 | DOI: 10.1007/s11099-011-0008-0

High-light-induced superoxide anion radical formation in cytochrome b6f complex from spinach as detected by EPR spectroscopy

M. Sang1,2, X. C. Qin1, W. D. Wang1, J. Xie3, X. B. Chen4, K. B. Wang1, J. P. Zhang5, L. B. Li1, T. Y. Kuang1
1 Institute of Hydrobiology, Ji'nan University, Guangzhou, P.R. China
2 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, P.R. China
3 Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, P.R. China
4 School of Bioscience and Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei Province, P.R. China
5 Department of Chemistry, Renmin University of China, Beijing, P.R. China

The generation of superoxide anion radical (O2 .-) in the cytochrome b 6 f complex (Cyt b 6 f) of spinach under high-light illumination was studied using electron paramagnetic resonance spectroscopy. The generation of O2 .- was lost in the absence of molecular oxygen. It was also suppressed in the presence of NaN3 and could be scavenged by extraneous antioxidants such as ascorbate, β-carotene, and glutathione. The results also indicate that O2 .-, which is produced under high-light illumination of the Cyt b 6 f from spinach, might be generated from a reaction involing 1O2, and the Rieske Fe-S protein could serve as the electron donor in the O2 .- production. The mechanism of photoprotection of the Cyt b 6 f complex by antioxidants is discussed.

Keywords: Cyt b6f; EPR; photoprotection; superoxide anion radical

Received: June 9, 2010; Accepted: January 14, 2011; Published: March 1, 2011Show citation

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Sang, M., Qin, X.C., Wang, W.D., Xie, J., Chen, X.B., Wang, K.B., ... Kuang, T.Y. (2011). High-light-induced superoxide anion radical formation in cytochrome b6f complex from spinach as detected by EPR spectroscopy. Photosynthetica, 49(1), 48-54. doi: 10.1007/s11099-011-0008-0.
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