Photosynthetica, 2011 (vol. 49), issue 1

Photosynthetica 2011, 49(1):154-160 | DOI: 10.1007/s11099-011-0011-5

Growth and photosynthetic characteristics of Nigella sativa L. as affected by presowing seed treatment with kinetin

S. H. Shah1,*
1 Plant Physiology Division, Department of Botany, Aligarh Muslim University, Aligarh, India

Black cumin (Nigella sativa L.) seeds were surface-sterilized and soaked for 5, 10, or 15 h in 1, 10, or 100 μM aqueous solution of kinetin (KIN). The potted plants were then analyzed at 30, 50, and 70 days after emergence (DAE) for dry mass (DM), leaf area (LA), chlorophyll (Chl) content, stomatal conductance (g s), carbonic anhydrase (CA), and nitrate reductase (NR) activity, total protein content, and net photosynthetic rate (P N). Capsule number and seed yield were determined at harvest (90 DAE). Treatment with the growth regulator was found to appreciably enhance all the determinants, with most prominent results being obtained following 10-h soaking with 10 μM KIN, in which case the values for DM, LA, P N, CA and NR activity, and seed yield were elevated by 55, 63, 43, 38, 29, and 23% respectively over the control at the 50-day stage.

Keywords: carbonic anhydrase; chlorophyll; kinetin; Nigella sativa; nitrate reductase; photosynthetic rate; protein; seed treatment; stomatal conductance

Received: August 19, 2010; Accepted: February 4, 2011; Published: March 1, 2011Show citation

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Shah, S.H. (2011). Growth and photosynthetic characteristics of Nigella sativa L. as affected by presowing seed treatment with kinetin. Photosynthetica, 49(1), 154-160. doi: 10.1007/s11099-011-0011-5.
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    References

    1. Afzal, I., Basra, M.A, Iqbal, A.: The effects of seed soaking with plant growth regulators on seedling vigor of wheat under salinity stress. - J. Stress Physiol. Biochem. 1: 6-14, 2005.
    2. Aldesuquy, H.S., Baka, Z.A.M.: Interactive effect of seawater and plant hormones on the pigment content and chloroplast ultrastructure of wheat flag leaf. The 6th conference of Egypt. - Bot. Soc. 1: 51-64, 1998.
    3. Aldesuquy, H.S., Gaber, A.M.: Effect of growth regulating substances on Vicia faba plants irrigated by sea-water. 1. Leaf area, pigment content and photosynthetic activity. - J. Environ. Sci. 4: 291-009, 1992.
    4. Arteca, R.N., Dong, C.N.: Stimulation of photosynthesis by application of phytohormones to root systems of tomato plants. - Photosynth. Res. 2: 243-249, 1981. Go to original source...
    5. Chernyad'ev, I.I., Monakhova, O.F.: The activity and content of ribulose-1,5-phosphate carboxylase/oxygenase in wheat plants as affected by water stress and kartolin-4. - Photosynthetica 35: 603-610, 1998. Go to original source...
    6. Dwivedi, R.S., Randhawa, N.S.: Evaluation of a rapid test for the hidden hunger of zinc in plants. - Plant Soil 40: 445-451, 1974. Go to original source...
    7. El-Shihaby, O.A., Alla, M.M.N., Younis, M.E., El-Bastawisy, Z.M.,: Effect of kinetin on photosynthetic activity and carbohydrate content in waterlogged or seawater-treated Vigna sinensis and Zea mays plants. - Plant Biosystems 136: 277-290, 2002. Go to original source...
    8. Faiss, M., Zalubilová, J., Strnad, M., Schmülling, T.: Conditional transgenic expression of the IPT gene indicates a function for cytokinins in paracrine signalling in whole tobacco plants. - Plant J. 12: 401-415, 1997. Go to original source...
    9. Fatima, Z., Bano, A., Sial, R., Aslam, M.: Response of chickpea to plant growth regulators on nitrogen fixation and yield. - Pak. J. Bot. 40: 2005-2013, 2008.
    10. Gomez, K.A., Gomez, A.A.: Statistical Procedures for Agricultural Research. 2nd Ed. - John Wiley & Sons, New York 1984.
    11. Hutchison, C.E., Kieber, J. J.: Cytokinin signalling in Arabidopsis. - Plant Cell 14: S47-S59, 2002. Go to original source...
    12. Iqbal, M., Ashraf, M.: Presowing seed treatment with cytokinins and its effect on growth, photosynthetic rate, ionic levels and yield of two wheat cultivars differing in salt tolerance. - J. Integrative Plant Biol. 47: 1315-1325, 2005. Go to original source...
    13. Ismail, A.M.: Physiological studies on the influence of cytokinin or GA3 in the alleviation of salt stress in sorghum plants. - Acta Agr. Hungarica 51: 371-380, 2003. Go to original source...
    14. Jablonski, J.R., Skoog, F.: Cell enlargement and cell division in excised tobacco pith tissue. - Physiol. Plant. 7: 16-24, 1954. Go to original source...
    15. Jaworski, E.G.: Nitrate reductase assay in intact plant tissue. - Biochem. Biophys. Res. Commun. 43: 1274-1279, 1971. Go to original source...
    16. Khan, A.A., Huang, X.-L.: Synergistic enhancement of ethylene production and germination with kinetin and 1-aminocyclopropane-1-carboxylic acid in lettuce seeds exposed to salinity stress. - Plant Physiol. 87: 847-852, 1988. Go to original source...
    17. Lechowski, Z.: Stomatal response to exogenous cytokinin treatment of the hemiparasite (Melampyrum arvense L.) before and after attachment to the host. - Biol. Plant. 39: 13-21, 1997. Go to original source...
    18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with Folin-phenol reagent. - J. Biol. Chem. 193: 265-275, 1951.
    19. Lu, J.L., Ertl, J.R., Chen, C.M.: Cytokinin enhancement of the light induction of nitrate reductase transcript levels in etiolated barley leaves. - Plant Mol. Biol. 14: 585-594, 1990. Go to original source...
    20. Lu, J.L., Ertl, J.R., Chen, C.M.: Transcriptional regulation of nitrate reductase mRNA levels by cytokinin-abscisic acid interactions in etiolated barley leaves. - Plant Physiol. 98: 1255-1260, 1992. Go to original source...
    21. MacKinney, G.: Absorption of light by chlorophyll solutions. - J. Biol. Chem. 140: 315-322, 1941.
    22. Moore, T.C.: Biochemistry and Physiology of Plant Hormones. 2nd Ed. - Springer-Verlag, New York - Heidelberg - Berlin - London - Paris - Tokyo - Hong Kong 1989.
    23. Pospíšilová, J., Synková, H., Rulcová, J.: Cytokinins and water stress. - Biol. Plant. 43: 321-328, 2000.
    24. Premabatidevi, R.K.: Effect of IAA, GA3 and kinetin on nitrate reductase and nitrite reductase in the leaves of a tree legume. - Indian J. Plant Physiol. 3: 97-101, 1998.
    25. Sawan, Z.M., Mohamed, A.A., Sakr, R.A., Tarrad, A.M.: Effect of kinetin concentration and methods of application on seed germination, yield components, yield and fiber properties of the Egyptian cotton (Gossypium barbadense). - Environ. Exp. Bot. 44: 59-68, 2000. Go to original source...
    26. Seth, A.K., Wareing, P.F.: Interactions between auxins, gibberrelins, and kinins in hormone-directed transport. - Life Sci. 3: 1483-1486, 1964. Go to original source...
    27. Shah, S.H.: Morphophysiological Response of Black Cumin (Nigella sativa L.) to Nitrogen, Gibberellic acid and Kinetin Application. - Ph.D. Thesis, Aligarh Muslim Univ., Aligarh 2004.
    28. Shah, S.H.: Effect of kinetin spray on growth and productivity of black cumin plants. - Russ. J. Plant Physiol. 54: 702-705, 2007. Go to original source...
    29. Shah, S.H.: Kinetin improves photosynthetic and antioxidant responses of Nigella sativa to counteract salt stress. - Russ. J.. Plant Physiol. 58: 454-459, 2011. Go to original source...
    30. Shah, S.H., Ahmad, I., Samiullah: Responses of Nigella sativa L. to foliar application of gibberellic acid and kinetin. - Biol. Plant. 51: 563-566, 2007. Go to original source...
    31. Sugiharto, B., Sugiyama, T.: Effects of nitrate and ammonium on gene expression of phosphoenolpyruvate carboxylase and nitrogen metabolism in maize leaf tissue during recovery from nitrogen stress. - Plant Physiol. 98: 1403-1408, 1992. Go to original source...
    32. Synková, H., Wilhelmová, N., Šesták, Z., Pospíšilová, J.: Photosynthesis in transgenic plants with elevated cytokinin contents. - In: M. Pessarakli (ed.), Handbook of Photosynthesis. Pp. 541-552. Marcel Dekker, New York - Basel - Hong Kong 1997.
    33. Weyers, J.D.B., Paterson, N.W.: Plant hormones and the control of physiological processes. - New Phytol. 152: 375-407, 2001. Go to original source...
    34. Wingler, A., von Schaewen, A., Leegood, R.C., Lea, P.J., Quick, W.P.: Regulation of leaf senescence by cytokinin, sugars, and light. Effects on NADH-dependent hydroxypyruvate reductase - Plant Physiol. 116: 329-335, 1998. Go to original source...

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