Tomáš Špringer, Ph.D.

Research interests:

Study of nucleic acids and their analogues significant in therapies based on suppression of genetic expression.

Development of microfluidics for SPR biosensors.

Application of gold nanoparticles for ultra-sensitive SPR biosensors.

Surface plasmon resonance (SPR) biosensors.

Education:

2015 - today: Postdoctoral fellow

2006 - 2015: PhD study, Charles University, Faculty of Mathematics and Physics (Biophysics) at the Institute of Photonics and Electronics, dpt. of Optical Sensors

Thesis: In situ study of interactions of nucleic acids significant in genetic expression and therapy based on its suppression
Supervisor: Prof. Josef Stepanek, PhD.

2001 - 2006: Master of Science, Charles University, Faculty of Mathematics and Physics (Biophysics)

Thesis: Surface-enhanced photophysical processes of organic molecules in metal nanoparticles
Supervisor: Doc. RNDr. Marek Procházka, Dr.

Awards:

2nd poster award, Europt(r)ode X 2010, Prague: Real-time monitoring of biomolecular interactions in blood plasma using surface plasmon resonance sensor

T. Špringer, M. Piliarik, J. Homola

Young Scientist Prize of the CSS IEEE EMB 2016 for the best poster presentation:

Functional gold nanoparticles for highly sensitive optical detection of cancer biomarker

T. Špringer, X.C. Song, M.L. Ermini, J. Lamačová, J. Homola

3BIO: Biophotonics and Bioelectronics for Biomedicine 2016, IPE CAS, Prague, Czech Republic

Publications:

Book chapters:

1. P. Adam, M. Piliarik, H. Šípová, T. Špringer, M. Vala, J. Homola: Surface Plasmons for Biodetection, in Photonic Sensing: Principles and Applications for Safety and Security Monitoring, editors W. J. Bock, J., X. Gaozhi, John Wiley & Sons, 2012, 1-58.

Journal papers:

1. E. Hemmerová, T. Špringer, Z. Krištofiková and J. Homola: Ionic environment affects biomolecular interactions of amyloid-β: SPR biosensor study, International Journal of Molecular Sciences, 21, 9727 (2020).
2. E. Hemmerová, T. Špringer, Z. Krištofiková, J. Homola: Study of biomolecular interactions of mitochondrial proteins related to Alzheimer's disease: toward multi-interaction biomolecular processes, Biomolecules, 10, Article Number: 1214 (2020).
3. T. Špringer, E. Hemmerová, G. Finocchiaro, Z. Krištofiková, M. Vyhnálek, J. Homola: Surface plasmon resonance biosensor for the detection of tau-amyloid beta complex, Sensors and Actuators B-Chemical, 316, Article Number: 128146 (2020).
4. Z. Krištofiková, T. Špringer, E. Gedeonová, A. Hofmannová, J. Ríčný, L. Hromadková, M. Vyhnálek, J. Laczo, T. Nikolai, J. Hort, T. Petrásek, A. Stuchlík, K. Valeš, J. Klaschka,  J. Homola: Interactions of 17β-Hydroxysteroid Dehydrogenase Type 10 and Cyclophilin D in Alzheimer's Disease, Neurochemical Research, 45, 915–927(2020).
5. N. S. Lynn, T. Špringer, J. Slabý, B. Špačková, M. Gráfová, M. L. Ermini, J. Homola: Analyte transport to micro- and nano-plasmonic structures, Lab on a Chip, 19, 4117-4127 (2019).
6. E. Hemmerová, T. Špringer, Z. Krištofiková, J. Homola: In vitro study of interaction of 17β-hydroxysteroid dehydrogenase type 10 and cyclophilin D and its potential implications for Alzheimer’s disease, Scientific Reports, 9, Article number 16700 (2019). 
7. M. Bocková, J. Slabý, T. Špringer, J. Homola: Advances in surface plasmon resonance imaging and microscopy and their biological applications, Annual Review of Analytical Chemistry, 12, 151–176 (2019).
8. M. L. Ermini, X. Chadtova Song, T. Špringer, J. Homola: Peptide functionalization of gold nanoparticles for the detection of carcinoembryonic antigen in blood plasma via SPR-based biosensor, Frontiers in Chemistry, doi: 10.3389/fchem.2019.00040 (2019)
9. T. Špringer, X. Chadtová Song, M. L. Ermini, J. Lamačová, J. Homola: Functional gold nanoparticles for optical affinity biosensing, Analytical and Bioanalytical Chemistry, 409, 4087–4097 (2017)
10. M. Kalousová, S. Dusilová-Sulková, A. Kuběna, O. Zakiyanov, K Levová, M. Bocková, E. Gedeonová, X. Chadtová Song, M. L. Ermini, T. Špringer, J. Homola, V. Tesař, and T. Zima:, 42, 509-518 (2017).
11. M. Bocková, J. Slabý, T. Špringer, J. Homola: Advances in Surface Plasmon Resonance Imaging and Microscopy and Their Biological Applications, Annual Review of Analytical Chemistry, 12, 151–176 (2019).
12. H. Vaisocherová-Lísalová, F. Surman, I. Víšová, M. Vala, T. Špringer, M. L. Ermini, H. Šípová, P. Šedivák, M. Houska, T. Riedel, O. Pop-Georgievski, E. Brynda, J. Homola: Copolymer Brush-Based Ultralow-Fouling Biorecognition Surface Platform for Food Safety, Analytical Chemistry, 88, 10533–10539 (2016).
13. H. Vaisocherová-Lísalová, I. Víšová, M. L. Ermini, T. Špringer, X. Song, J. Mrázek, J. Lamačová, N. S. Lynn Jr., P. Šedivák, and J. Homola: Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples, Biosensors and Bioelectronics, 80, 84–90 (2016).
14. M. Bocková, T. Špringer, I. Nečasová, J. Nunvar, B. Schneider, and J. Homola: Monitoring RAYT activity by surface plasmon resonance biosensor, Analytical and Bioanalytical Chemistry, 407, 3985-3993 (2015).
15. H. Vaisocherová, I. Víšová, M. Bocková, T. Špringer, M. L. Ermini, X. Song, Z. Krejčík, L. Chrastinová, O. Pastva, K. Pimková, M. Dostálová Merkerová, J. E. Dyr, and J. Homola: Rapid and sensitive detection of multiple MicroRNAs in cell lysate by low-fouling surface plasmon resonance biosensor, Biosensors and Bioelectronics, 70, 226–231 (2015).
16. T. Špringer, M. L. Ermini, B. Špačková, J. Jabloňků, J. Homola: Enhancing sensitivity of SPR biosensors by functionalized gold nanoparticles: size matters, Analytical Chemistry, 86, 10350−10356 (2014)
17. H. Šípová, T. Špringer, D. Rejman, O. Šimák, M. Petrová, P. Novák, Š. Rosenbergová et al.: 5′-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes. Nucleic acids research (2014). link
18. T. Špringer, M. Bocková, J. Homola: Label-Free Biosensing in Complex Media: A Referencing Approach.  Analytical Chemistry, 85 (2013), 5637–5640. link
19. T. Špringer, J. Homola: Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma. Analytical and Bioanalytical Chemistry, 404 (2012), 2869-2875. link
20. H. Šípová, T. Špringer, J. Homola: Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53. Analytical and Bioanalytical Chemistry, 399 (2011), 2343-2350. link
21. T. Špringer, H. Šípová, H. Vaisocherová, J. Štepánek, J. Homola: Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance study. Nucleic Acids Research, 38 (2010), 7343-7351. link
22. T. Špringer, M. Piliarik, J. Homola: Surface plasmon resonance sensor with dispersionless microfluidics for direct detection of nucleic acids at the low femtomole level. Sensors and Actuators B - Chemical, 145 (2010), 588-591. link
23. T. Špringer, M. Piliarik, J. Homola: Real-time monitoring of biomolecular interactions in blood plasma using a surface plasmon resonance biosensor. Analytical and Bioanalytical Chemistry, 398 (2010),1955-1961. link
24. H. Vaisocherová, J. Snášel, T. Špringer, H. Šípová, I.  Rosenberg, J. Štěpánek, J. Homola: Surface plasmon resonance study on HIV-1 integrase strand transfer activity. Analytical and Bioanalytical Chemistry, 393 (2009), 1165–1172. link

Konferenční příspěvky:

1. Z. Kristofikova, A. Hofmannova, L. Hromadkova, E. Gedeonova, T. Springer, M. Bockova, J. Homola: Interactions of 17beta­hydroxysteroid dehydrogenase type 10 and cyclophilin D in Alzheimer disease. FEBS 2018, Praha
2. M. Kalousová, S. Dusilová-Sulková, A. Kuběna, O. Zakiyanov, K. Levová, M. Bocková, E. Gedeonová, X. Chadtová Song, M. L. Ermini, T. Špringer, J. Homola, V. Tesař, T. Zima: Pregnancy-associated plasma protein A2 in hemodialysis patients: significance for prognosis. 54th ERA-EDTA Congress, 2017
3. T. Špringer, X.C. Song, M.L. Ermini, J. Lamačová, J. Homola : Functional gold nanoparticles for highly sensitive optical detection of cancer biomarker. 3BIO: Biophotonics and Bioelectronics for Biomedicine 2016, IPE CAS, Praha, Young Scientist Prize of the CSS IEEE EMB za nejlepší prezentaci posteru 
4. T. Špringer, X.C. Song, M.L. Ermini, J. Lamačová, J. Homola: Functional gold nanoparticles for highly sensitive optical detection of cancer biomarker T. Biosensors 2016, Gottenburg, Švédsko, poster 
5. E. Brynda, H. Vaisocherová-Lísalová, F. Surman, I. Víšová, H. Šípová, T. Špringer, M. L. Ermini, O. Pop-Georgievski, M. Houska, J. Homola: Ultra-low fouling biorecognition platform for label-free biosensing in complex samples. Biosensors 2016, Gottenburg, Švédsko, poster 
6. H. Vaisocherová-Lísalová, I. Víšová, H. Šípová, T. Špringer, M. L. Ermini, F. Surman, O. Pop-Georgievski, M. Houska, E. Brynda, J. Homola: Challenging Functionalization of Ultra-low Fouling Dual-Functional Co-polymer Surfaces for Biosensing in Undiluted Media. Europtrode XIII 2016, Graz, Rakousko, oral prezentation
7. T. Špringer, M. L. Ermini, X. Chadtová Song, J. Homola: Functionalized nanoparticles for highly sensitive optical detection of cancer biomarkers in blood plasma samples. Nanocon 2015, Brno, oral presentation
8. H. Vaisocherová, I. Víšová, K. Mrkvová, H. Šípová, T. Špringer, M.L. Ermini, F. Surman, C. Rodriguez-Emmenegger, E. Brynda, J. Homola: Ultra-low Fouling Biosensor for Rapid Detection of Multiple Bacterial Pathogens in Crude Food Samples. TechConnect World Innovation Conference and Expo 2015, Washington, USA
9. M. Bocková, N. S. Lynn Jr., T. Špringer, J. Homola: SPR imaging-based microarray for enhanced multianalyte detection in blood plasma. Biodetection and Biosensors 2015, Berlin, Německo
10. H. Vaisocherová, H. Šípová, I. Víšová, X. Song, K. Pimková, J.E. Dyr, J. Homola: An ultra-low fouling high-capacity DNA array for direct detection of miRNA at subnanomolar levels in celllysate.  Biosensors 2014, Australia
11. H Vaisocherova, I. Visova, T. Springer, M. L. Ermini, V. Sevcu, K. Mrkvova, X. Song, P. Adam, P. Kvasnicka, J. Homola: Advancing ultra-low fouling surface chemistries for label-free biosensors for food safety. Europt(r)ode XII 2014, Athény, Řecko 
12. M. L. Ermini, T. Špringer, J. Homola: Functionalization of gold spherical nanoparticles for cancer marker detection by surface plasmon resonance based biosensors. 4th International Colloids Conference, Surface Design & Engineering, 15-18 June 2014, Madrid, Španělsko
13. Size effect of neutravidin-coated gold nanoparticles on the enhanced SPR sensor response in the detection of carcinoembryonic antigen, T. Špringer, M. L. Ermini, P. Kvasnička, B. Špačková, J. Homola - Advances in Biodetection and Biosensors 2014, Berlin
14. Functionalized nanoparticles for the amplification of sensor response in SPR biosensors, T. Špringer, J. Homola - Europt(r)ode XI 2012, Barcelona
15. Single-DNA binding event detection in amplified micro-SPR surface plasmon resonance sensor, P. Kvasnička, T. Špringer, J. Homola - Europt(r)ode XI 2012, Barcelona
16. Real-time monitoring of biomolecular interactions in blood plasma using surface plasmon resonance sensor, T. Špringer, M. Piliarik, J. Homola- Europt(r)ode X 2010, Prague- 2. místo v soutěži o nejlepší poster
17. Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53, H. Šípová, T. Špringer, J. Homola- Europt(r)ode X 2010, Prague
18. Compact multi-channel surface plasmon resonance sensor for rapid detection of bacterial pathogens M. Vala, H. Šípová, T. Špringer, K. Chadt, M. Piliarik, J. Homola- Europt(r)ode X 2010, Prague
19. T. Špringer, H. Šípová, H. Vaisocherová, J. Štěpánek, J. Homola: 2D mapping of simultaneous magnesium and sodium effect on DNA hybridization via surface plasmon resonance sensor. 12th ECSBM (European Conference on the Spectroscopy of Biological Molecules), Bobigny, France, 2007