Visualization of collagen production in osteogenic cells cultivated on diamond films

Abstract

Nanocrystalline diamond films (NCD) with various physicochemical properties (i.e., roughness and topography, wettability and electrical conductivity achieved by boron doping) will be grown on silicon and titanium substrates by microwave plasma-enhanced chemical vapor deposition. The films will be used as substrates for the adhesion and growth of bone-derived cells and for their phenotypic maturation, manifested particularly by production of collagen, a major protein of the bone extracellular matrix.