This transporter is localized in the membranes of vacuoles and its activity enables Yeast cells to cope with sudden changes in osmotic pressure. This transporter is homologous to the proteins of CCC (cation-chloride-cotransporter) family that are very important in mammalian cells and whose dysfunction leads to serious diseases. Consequently, we have expressed one of the human CCC proteins in cells and characterized its properties.

Petrezsélyová S., Zimmermannová O., Sychrová H. Vhc1, a novel transporter belonging to the family of electroneutral cation-Cl^- cotransporters, participates in the regulation of cation content and morphology of Saccharomyces cerevisiae vacuoles. BBA-Biomembranes 1828: 623-631 (2013).

Petrezsélyová S., Dominguez A., Herynková P., Macias J.F., Sychrová H. Human NKCC2 cation–Cl– co-transporter complements lack of Vhc1 transporter in yeast vacuolar membranes. Yeast 30: 395–402 (2013).