Arabidopsis thaliana AtTFIIB1 gene displays alternative splicing under different abiotic stressesOriginal Papers

J.A. MIRANDA-RÍOS, J.A. RAMÍREZ-TRUJILLO, D.J. JAIME-GALLARDO, N.S. HERNÁNDEZ-BUENO, M. RAMÍREZ-YÁÑEZ, G. ITURRIAGA, R. SUÁREZ-RODRÍGUEZ

Biologia plantarum 65:255-264, 2021 | DOI: 10.32615/bp.2021.022  

In Arabidopsis, there are 14 TFIIB-like proteins that have been phylogenetically categorized into the TFIIB, BRF, and Rrn7/TAF1B/MEE12 subfamilies. The TFIIB transcription factor (TF) subfamily plays a key role in the regulation of gene expression in eukaryotes. To identify the expression patterns of some members of the TFIIB and BRF subfamilies in A. thaliana, different approaches were carried out to determine the possible functions of some of these transcription factors. Through an in silico analysis, we identified possible cis-acting regulatory elements in the promoter regions that drive the expression of transcription...

Methyl jasmonate elicitation of common bean seedlings induces nucleotidase activity and the expression of several nucleotidase genes in radiclesOriginal Papers

G. GALVEZ-VALDIVIESO, M. GARMENDIA-CALVO, M. PINEDA, P. PIEDRAS

Biologia plantarum 65:246-254, 2021 | DOI: 10.32615/bp.2021.017  

Nucleotides are the basic elements of the genetic material, participate in bio-energetic processes, are cofactors and components of secondary metabolites, etc. Nucleotide hydrolases (nucleotidases) are phosphatases that remove the 5'-phosphate group from the nucleotides and play a crucial role in nucleotide metabolism. In this study, genes encoding putative nucleotidases in Phaseolus vulgaris have been identified, and the effect of methyl jasmonate (MeJA) on both nucleotidase activity and gene expression has been addressed. The predicted nucleotidase peptides include the conserved domains characteristic of the haloacid dehalogenase-like...

Obituary - Otília GašparíkováObituary

M. Čiamporová

Biologia plantarum 65:2021  

The complete chloroplast genome of Tamarix ramosissima and comparative analysis of Tamaricaceae speciesOriginal Papers

L. WANG, L. WANG, Z.-H. GUO

Biologia plantarum 65:237-245, 2021 | DOI: 10.32615/bp.2021.014  

Tamarix ramosissima is a deciduous shrub that resides in arid and semi-arid regions. Although of ecological and medicinal values, some Tamarix species are considered invasive as they have dominated the riparian zones of dryland in some parts of the world. Here, the complete chloroplast (cp) genome of T. ramosissima was sequenced and analyzed, showing a size of 156 150 bp and a GC content of 36.5 %. The plastome displayed a typical quadripartite structure, consisting of a pair of inverted repeat (IR) regions of 26 554 bp, separated by a large single copy (LSC) region of 84 795 bp, and a small single copy (SSC) region of 18 247 bp. The...

The effect of Fusarium culmorum inoculation and deoxynivalenol application on proteome response in wheat cultivars Sumai 3 and SW KadriljOriginal Papers

K. KOSOVÁ, J. CHRPOVÁ, J. ŠANTRŮČEK, R. HYNEK, M. KLÍMA, I.T. PRÁŠIL, P. VÍTÁMVÁS

Biologia plantarum 65:221-236, 2021 | DOI: 10.32615/bp.2021.010  

Fusarium head blight (FHB) represents a damaging disease of cereal spikes caused by mycotoxin-producing Fusarium fungi revealing adverse effects on grain quality and yield. Chinese spring wheat (Triticum aestivum L.) cv. Sumai 3 represents a major source of FHB resistance thanks to Fhb1 QTL on 3BS chromosome conferring resistance to FHB and encoding a lectin-like protein. The aim of our study lied in a comparison of proteome response to Fusarium culmorum inoculation and mycotoxin deoxynivalenol (DON) application at 10 d after inoculation (dai) in spikes of resistant wheat cultivar Sumai 3 and susceptible wheat cultivar SW...

Transcriptome comparison of physiological divergence between two ecotypes of Portulaca oleraceaOriginal Papers

M. WU, S. FU, W. JIN, W.Z. XIANG, W.C. ZHANG, L. CHEN

Biologia plantarum 65:212-220, 2021 | DOI: 10.32615/bp.2021.012  

According to the survey, wild purslane (Portulaca oleracea L.) has two different ecotypes, one with the red stem living in an arid environment and one with the green stem living in a humid environment. In order to explore the physiological response strategies of plants to environmental changes, these two ecotypes of purslane were selected as experimental materials. Physiological indices were determined and transcriptome analysis was carried out to screen the differentially expressed genes (DEGs) from two ecotypes of purslane. The results showed that the content of soluble sugars, proline, anthocyanins, and chlorophylls was significantly different,...

Physiological and biochemical changes during graft union formation in Carya illinoinensisOriginal Papers

W.C. SU, H.Y. HE, Z.Z. LIU, Z.H. MO, F. CAO, F.R. PENG

Biologia plantarum 65:203-211, 2021 | DOI: 10.32615/bp.2021.023  

To reveal the physiological and biochemical mechanism underlying graft union formation in pecan (Carya illinoinensis), dynamic changes in content of nutrients, tannin, and phytohormones together with key enzyme activities were investigated 0, 3, 5, 7, 10, 14, 18, 22, 31, and 40 d after grafting (DAG), in homograft unions. During graft union formation, peroxidase (POD) activity increased at 7 - 10 DAG compared with that at 0 DAG. Polyphenol oxidase (PPO) activity was higher in grafted than ungrafted pecan seedlings (control) at 22 DAG, which was similar to POD activity. The tannin content exhibited a decreasing trend with grafting relative to...

Effects of chlorsulfuron and cadmium on metabolites of maize seedlingsOriginal Papers

L. ZHAO, S. YAN, M. WANG, H. ZHANG, X. SHI, J. ZHANG, Y. DENG, L. ZHAO

Biologia plantarum 65:193-202, 2021 | DOI: 10.32615/bp.2021.013  

The impact of persisting herbicide residues on succeeding crops is of great concern to farmers because even the presence of very low concentrations can inhibit growth of crop and cause crop reduction. Furthermore, wastewater irrigation can lead to cadmium accumulation in soils. Thus, the co-occurrence of low amounts of herbicide residues and cadmium within agricultural fields are difficult to avoid. How the combination of these two pollutants affect plant metabolites remains to be elucidated and thus warrants investigation. Maize seeds were planted in soil that had been sprayed with chlorsulfuron and Cd, then we studied the effects of exposure to the...

Spermine alleviates heat-induced senescence in creeping bentgrass by regulating water and oxidative balance, photosynthesis, and heat shock proteinsOriginal Papers

L.L. LIANG, Y.Q. CAO, D. WANG, Y. PENG, Y. ZHANG, Z. LI

Biologia plantarum 65:184-192, 2021 | DOI: 10.32615/bp.2021.008  

Spermine (SPM) is involved in response to abiotic stress in plants, but the potential role of SPM in regulating senescence has not been well documented. Objectives of this study were to examine the effect of changes in endogenous polyamines (PAs) by SPM application on improving heat tolerance of creeping bentgrass (Agrostis stolonifera) and explore the SPM-regulated senescence associated with alterations of water and oxidative balance, photosynthesis, and heat shock proteins under heat stress. The results showed that persistent high temperature caused severe oxidative damage and significant decreases in chlorophyll (Chl) content, photosynthetic...

Purification, immunological, and functional characterization of MxFIT in Malus xiaojinensisOriginal Papers

L.L. YIN, X.L. CHEN, S.L. MA, F. LI, F. ZHOU, Y. WANG

Biologia plantarum 65:177-183, 2021 | DOI: 10.32615/bp.2021.004  

MxFIT is a FER-like iron deficiency induced transcriptional factor in Malus xiaojinensis. Here, we described the heterologous expression of MxFIT in Escherichia coli BL21 (DE3) host cells. The E. coli harboring the recombinant construct pET-MxFIT was efficiently induced to express the MxFIT protein at a high level and the optimal profile for MxFIT expression was investigated. By inoculating a New Zealand rabbit with purified MxFIT-His fusion protein, a high specific anti-MxFIT antiserum was achieved. Western blot analysis showed MxFIT protein expression was induced in roots when iron supply was limiting and was inhibited...

Lysigenous aerenchyma formation: responsiveness to waterlogging in oil palm rootsOriginal Papers

S. NUANLAONG, S. WUTHISUTHIMATHAVEE, P. SURANINPONG

Biologia plantarum 65:167-176, 2021 | DOI: 10.32615/bp.2021.002  

Oil palm (Elaeis guineensis Jacq.) responds to waterlogging stress by induction of lysigenous aerenchyma tissues, which facilitates the flow of oxygen through their root tissues for survival under waterlogged conditions. Thus, the morphological and genetic adaptation involved in lysigenous aerenchyma formation in the roots of the oil palm genotype Deli × Calabar under waterlogging stress was evaluated. This study found the highest number of dead cells after waterlogging stress for 2 d in the secondary root, while the percentage of root porosity was increased with increasing of time in both roots, especially at 1.0 - 2.0 cm from...

Ectopic expression of GmNHX3 and GmNHX1, encoding two Glycine max Na⁺/H⁺ vacuolar antiporters, improves water deficit tolerance in Arabidopsis thalianaOriginal Papers

E.M. PARDO, L. TOUM, L.S. PÉREZ-BORROTO, L. FLEITAS, J.P. GALLINO, S. VIDAL MACHI, A.VOJNOV, A.P. CASTAGNARO, B. WELIN

Biologia plantarum 65:157-166, 2021 | DOI: 10.32615/bp.2021.003  

The importance of Na⁺/H⁺ antiporters in salt tolerance in plants has been demonstrated in many studies, but much less is known about their protective role during drought stress. To study their possible contribution to water deficit tolerance, two closely related soybean Na⁺/H⁺ antiporters belonging to the intracellular NHX exchanger protein family, GmNHX3 and GmNHX1, were evaluated in transgenic Arabidopsis thaliana. A. thaliana plants ectopically expressing GmNHX3 or GmNHX1 displayed a more drought-tolerant phenotype compared to wild-type plants, which was accompanied by an increase...

Physiological and transcriptomic analysis of Pinus massoniana seedling response to osmotic stressOriginal Papers

H. XU, X. GAO, C. YU

Biologia plantarum 65:145-156, 2021 | DOI: 10.32615/bp.2021.016  

Masson pine (Pinus massoniana Lamb.) is an important tree species of high economic value in southern China, but osmotic stress threatens its growth and development. In this study, physiological measurements and RNA-Seq analysis were used to clarify the physiological and molecular responses of P. massoniana under osmotic stress. Osmotic treatment caused cell membrane damage and reactive oxygen species (ROS) accumulation in the tree seedlings, but it also increased their antioxidant enzyme (superoxide dismutase, peroxidase, and catalase) activities and osmotic substances (soluble sugars, proline, and trehalose) content so as to adjust to...

Comparison of antioxidant enzyme activity and gene expression in two new spring wheat cultivars treated with salinityOriginal Papers

Y.L. XU, Y. ZHANG, J.M. LI, T.P. GAO, L.N. ZHANG, L.B. SI, Q. LI, G.Y. LI, Y.L. YANG

Biologia plantarum 65:131-144, 2021 | DOI: 10.32615/bp.2020.171  

This study aimed to analyze element content, antioxidative response, and related gene expression in two new wheat (Tritium aestivum L.) cultivars Longchun 30 and Longchun 27 when exposed to different NaCl concentrations. Low NaCl concentration (25 mM) promoted root growth and decreased malondialdehyde (MDA) content and relative conductivity (REC) in Longchun 30. Differently, higher salinity stress (100 and 200 mM NaCl) inhibited root growth and increased MDA content and REC in both cultivars. Under salt stress, the increment of Na content in the roots and leaves and the reduction of Ca content in the roots were more remarkable in Longchun 27...

Microtubule interaction of LICC1, a maize homologue of a component of the human muskelin/RanBPM/CTLH protein complexBrief Communications

M. MIQUEL, D. PAGÈS-VILA, C.M. VICIENT

Biologia plantarum 65:126-130, 2021 | DOI: 10.32615/bp.2020.168  

MRCTLH (muskelin/RanBPM/CTLH) is a protein complex found in humans (MRCTLH) that is involved in the regulation of numerous cellular processes, such as gluconeogenesis, cell signaling, development, nuclear extrusion, cell morphology, or stability of different proteins. According to genomic data, all eukaryotes have similar protein complexes. In yeast, a similar protein complex named GID was found to be involved in the regulation of gluconeogenesis. LICC1 is a maize protein whose sequence resembles that of TWA1 in humans and GID8 in yeast, which are central components of the MRCTLH and GID complexes. LICC1 contains three highly conserved protein domains,...

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanusOriginal Papers

K. NING, T. ZHOU, C.Z. JIANG, H.M. WU, J.L. JIANG, J. CHEN, Y.A. El-KASSABY, Y. MA

Biologia plantarum 65:118-125, 2021 | DOI: 10.32615/bp.2020.166  

Rapid propagation of plants by tissue culture is of great significance for large-scale production, molecular genetics research, and breeding. Currently, a rapid and high-efficient tissue culture protocol for Euonymus bungeanus is needed. To develop a propagation system for this species, we established a new regeneration system from leaves. Callus formation was induced on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm^-3 6-benzylaminopurine (6-BA) and 0.5 mg dm^-3 α-naphthalene acetic acid (NAA) and the induction rate almost reached 100 % under red radiation within 21 d. The medium for proliferation of adventitious...

Tolerance of Arabidopsis thaliana plants overexpressing grapevine VaSTS1 or VaSTS7 genes to cold, heat, drought, salinity, and ultraviolet irradiationOriginal Papers

Z.V. OGNEVA, O.A. ALEYNOVA, A.R. SUPRUN, Y.A. KARETIN, A.S. DUBROVINA, K.V. KISELEV

Biologia plantarum 65:111-117, 2021 | DOI: 10.32615/bp.2020.139  

Stilbene synthases (STS) are plant enzymes that are responsible for the biosynthesis of stilbenes, which are plant phenolic compounds with valuable biological properties. Stilbenes also play important roles in plant tolerance to biotic and abiotic stresses. Therefore, plants that overexpress STS genes can be more resistant to various stresses. This paper investigated the effects of STS gene overexpression in Arabidopsis thaliana (L.) Heynh. Columbia-0 plants on stilbene content and tolerance to the following abiotic stresses: low and high temperatures, salinity, drought, and ultraviolet irradiation (UV-B and UV-C). We used VaSTS1...

Topical application of double-stranded RNA molecules deriving from Tomato yellow leaf curl virus reduces cognate virus infection in tomatoOriginal Papers

O. MELITA, A. KALDIS, M. BERBATI, C. REPPA, M. HOLEVA, M. LAPIDOT, D. GELBART, P. OTTEN, A. VOLOUDAKIS

Biologia plantarum 65:100-110, 2021 | DOI: 10.32615/bp.2020.172  

Tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted single-stranded DNA (ssDNA) virus, causes the most important viral disease of tomato worldwide. TYLCV-mediated disease is mainly controlled via extensive insecticide sprays aiming at the whitefly vector. RNA-based vaccination was proven to be a non-transgenic approach leading to efficient plant virus control. In this work, double-stranded RNA (dsRNA) molecules deriving from sequences of the C4 and V2 genes of TYLCV-Mild were produced in vitro and topically applied onto tomato plants along with the virus (via agroinfiltration). DsC4 and dsV2 application reduced disease...

Influence of water shortage on apple seedling growth under different radiation compositionOriginal Papers

F.A. HAMANN, A. FIEBIG, G. NOGA

Biologia plantarum 65:88-99, 2021 | DOI: 10.32615/bp.2020.086  

Abiotic stresses strongly impair plant development and might impose detrimental effects particularly on seedlings. Irradiance and water deficit are relevant factors, which affect performance of young plants under controlled conditions. In our study, we investigated the influence of water shortage combined with different radiation sources - light emitting diodes (LED) and compact fluorescence lamps (CFL) - on physiological and biochemical parameters of young apple plants. Stress responses were assessed by fluorescence-based indices, while relative water, chlorophyll (Chl), and proline content served as reference parameters. The watering regime had a...

Genome‑wide identification and analysis of the trihelix transcription factors in sunflowerOriginal Papers

J. SONG, W.Y. SHEN, S. SHAHEEN, Y.Y. LI, Z.R. LIU, Z. WANG, H.B. PANG, Z. AHMED

Biologia plantarum 65:80-87, 2021 | DOI: 10.32615/bp.2021.006  

The trihelix genes encode plant-specific transcription factors, which play a vital role in plant morphological and developmental processes. However, information about the presence of trihelix genes in sunflower (Helianthus annuus L.) is scarce. Sunflower belongs to composite family and possesses strong drought and salt-alkali tolerance. In this study based on H. annuus genome data, we have identified and analyzed the trihelix genes with a complete description of their physical and chemical properties, phylogenetic relationships, motif composition, chromosome distribution, exon-intron structure, cis-acting elements,...

HSP70 plays an ambiguous role during viral infections in plantsReviews

V. HÝSKOVÁ, K. BĚLONOŽNÍKOVÁ, N. ČEŘOVSKÁ, H. RYŠLAVÁ

Biologia plantarum 65:68-79, 2021 | DOI: 10.32615/bp.2021.001  

Heat shock and almost all types of stresses associated with oxidative stress are accompanied by heat shock protein (HSP) expression. HSPs are involved in refolding denatured proteins and directing unrepairable proteins for degradation. Thus, under stress conditions, HSPs help to restore cellular balance. However, in virus-infected plants, HSP70 can have both positive and negative effects because viruses usually recruit HSP70. HSP70 can promote the replication and translation of the viral genome, the formation of viral replication complexes, and the propagation of viral particles from cell to cell and throughout the plant. HSP gene silencing...

Cloning and functional analysis of the promoter of the sesquiterpene synthase gene ASS1 in Aquilaria sinensis Original Papers

Y.H. XU, F.F. LV, P.W. SUN, M.H. TIAN, J.H. WEI

Biologia plantarum 65:60-67, 2021 | DOI: 10.32615/bp.2020.141  

Agarwood, the resin part of Aquilaria spp., is valued in medicine, perfumes, and incense. The most important components of agarwood are sesquiterpenes, which are produced only when a healthy tree is wounded. Agarwood sesquiterpene synthase 1 (ASS1) is one of key enzymes responsible for the biosynthesis of sesquiterpenes in Aquilaria sinensis (Lour.) Gilg, and it is a typical wound-inducible synthase. To elucidate its regulatory mechanism at the transcriptional level, a 978-bp sequence upstream of the translation initiation codon ATG of the promoter for ASS1 was cloned. Computational analysis revealed that this promoter contained...

Molecular cloning and functional analysis of the thioredoxin gene SikTrxh from Saussurea involucrataOriginal Papers

L. ZHANG, C. LIU, F.F. CHENG, X.Y. GUO, Y.X. LI, A.Y. WANG, J.B. ZHU

Biologia plantarum 65:47-59, 2021 | DOI: 10.32615/bp.2020.155  

Thioredoxins are oxidoreductases that help to maintain redox homeostasis in plants under abiotic stress. In this study, a new thioredoxin gene, SikTrxh, was cloned from Saussurea involucrata (Kar. & Kir.), a perennial herb that grows in the high alpine mountains of Central Asia. Bioinformatics analysis shows that the full-length cDNA of SikTrxh consisted of 565 bp with a 354-bp open reading frame and encoded a 117 amino acid protein. Using quantitative reverse transcription (RT) PCR, we found that the expression of the SikTrxh gene was induced by salt, cold, and drought stresses, suggesting that this protein played a...

Growth response of Oryza sativa seedlings to graphene oxide and its variability among genotypesOriginal Papers

Y. HE, H.M. WEI, S.J. LIU, Y.C. XU, Z.Y. ZHU, H. YAN, J.X. LI, Z.H. TIAN

Biologia plantarum 65:39-46, 2021 | DOI: 10.32615/bp.2020.124  

With the extensive utilization of graphene nanomaterials, they inevitably enter our environment. The potential phytotoxicity and environmental impact of graphene oxide (GO) have recently attracted much attention. We designed the experiment based on seed germination, seedling morphology, physio-biochemical properties, and antioxidant enzyme activities of five rice genotypes (9311, MH63, R527, K866, and Nipponbare) under six concentrations of GO (0, 5, 10, 50, 100, and 150 mg dm^-3). We studied the effects of different concentrations of GO on germination index (GI), shoot length (SL) and root length (RL), adventitious root number, shoot and...

Overexpression of the Panax ginseng MYB4 gene enhances stress tolerance in transgenic Arabidopsis thalianaOriginal Papers

W.H. LIAN, T.X. SUN, X.Y. MENG, R. SUN, F. HUI, Y.N. JIANG, Y. ZHAO

Biologia plantarum 65:27-38, 2021 | DOI: 10.32615/bp.2020.164  

The myeloblastosis (MYB) transcription factors are essential for plant stress responses. They can enhance plant tolerance to abiotic stresses (e.g., drought, salinity, and cold) via improved physiological and biochemical responses including the accumulation of metabolites. In this study, we constructed a Panax ginseng MYB4 (PgMYB4) gene expression vector and established the stable transgenic Arabidopsis thaliana lines to study the effects of this gene on plant stress tolerance. The germination rate and seedling taproot length were greater for the PgMYB4-overexpressing plants than for the wild-type plants. Accordingly,...

Ectopic expression of a wheat superoxide dismutase gene TaSOD5 enhances salt and oxidative stress tolerance in ArabidopsisOriginal Papers

Y. WANG, Q. LIU, Y. LIU, G. LI, G. XIA, M. WANG

Biologia plantarum 65:19-26, 2021 | DOI: 10.32615/bp.2020.147  

Superoxide dismutase (SOD) is a crucial reactive oxygen species (ROS) scavenger, which converts superoxide radical to H₂O₂, so it is thought to enhance abiotic stress tolerance by reducing ROS and thus avoiding oxidative damage. In this study, we isolated a salt- and oxidative stress-responsive copper-zinc (Cu/Zn) SOD encoding gene TaSOD5 from wheat. The ectopic overexpression of TaSOD5 in Arabidopsis thaliana increased total SOD and Cu/Zn SOD activities and enhanced tolerance to salt stress. Arabidopsis ectopically expressing TaSOD5 possessed a superior resistance to oxidative stress stimulated...

Genome-wide association study of low nitrogen tolerance traits at the seedling stage of rapeseedOriginal Papers

C.L. ZENG, H.P. WAN, X.M. WU, X.G. DAI, J.D. CHEN, Q.Q. JI, F. QIAN

Biologia plantarum 65:10-18, 2021 | DOI: 10.32615/bp.2020.144  

The large application of nitrogen fertilizer will cause soil deterioration and pollute the environment. Reduction of nitrogen inputs and maintaining high yields are therefore essential to ensure a more sustainable agriculture. However, little information is available about rapeseed (Brassica napus L.) low nitrogen tolerance. We evaluated low nitrogen tolerance of 304 rapeseed accessions at seedling stage and performed a genome-wide association study to detect low nitrogen tolerance-related quantitative trait loci. A natural population comprising 304 B. napus inbred lines was genotyped with a Brassica 60K Illumina Infinium...

EditorialEditorial

H. Plchová

Biologia plantarum 65:2021  

Molecular cloning and characterization of a novel gene MsKMS1 in Medicago sativaOriginal Papers

B. HAN, P. ZHANG, Z.-Q. ZHANG, Y.-F. WANG, T.-M. HU, P.-Z. YANG

Biologia plantarum 65:1-9, 2021 | DOI: 10.32615/bp.2020.059  

Vacuole membrane proteins play a critical role in the regulation of plant physiological processes including normal growth and development, and responses to stresses. The killing me slowly 1 (KMS1) gene that encodes a soluble N-ethylmaleimide-sensitive fusion attachment receptor (SNARE) domain-containing vacuole membrane protein was first reported in Arabidopsis. Currently, the function of KMS1 in other plants under stress is poorly understood. In this study, we report cloning, expression, and characterization of a novel KMS1 gene in alfalfa (Medicago sativa L.), designated MsKMS1 (GenBank accession No....

Future agroclimatic conditions and implications for European grasslandsSpecial Issue: Festulolium

M. TRNKA, J. BALEK, M.A. SEMENOV, D. SEMERÁDOVÁ, M. BĚLÍNOVÁ, P. HLAVINKA, J.E. OLESEN, J. EITZINGER, A. SCHAUMBERGER, P. ZAHRADNÍČEK, D. KOPECKÝ, Z. ŽALUD

Biologia plantarum 64:865-880, 2020 | DOI: 10.32615/bp.2021.005  

Grasslands play a significant role in livestock fodder production and thus, contribute to food security worldwide while providing numerous additional ecosystem services. However, how agroclimatic conditions and adverse weather events relevant for grasslands will change across the European grassland areas has not been examined to date. Using a single reference setup for soil and management over 476 European sites defined by climate stations, we show the probability of eight selected adverse weather events with the potential to significantly affect grassland productivity under climate change and how these events vary regionally across Europe. Changes...