Photosynthetica, 2008 (vol. 46), issue 1

Photosynthetica 2008, 46(1):127 | DOI: 10.1007/s11099-008-0020-1

Partitioning of photosynthetic electron flow between CO2 assimilation and O2 reduction in sunflower plants under water deficit

W. Tezara1,*, S. Driscoll2, D. W. Lawlor2
1 Instituto de Biología Experimental, Universidad Central de Venezuela, Caracas, Venezuela
2 Biochemistry & Physiology Department, IACR-Rothamsted, Harpenden, Hertfordshire, UK

In sunflower (Helianthus annuus L.) grown under controlled conditions and subjected to drought by withholding watering, net photosynthetic rate (P N) and stomatal conductance (g s) of attached leaves decreased as leaf water potential (Ψw) declined from -0.3 to -2.9 MPa. Although g s decreased over the whole range of Ψw, nearly constant values in the intercellular CO2 concentrations (C i) were observed as Ψw decreased to -1.8 MPa, but C i increased as Ψw decreased further. Relative quantum yield, photochemical quenching, and the apparent quantum yield of photosynthesis decreased with water deficit, whereas non-photochemical quenching (qNP) increased progressively. A highly significant negative relationship between qNP and ATP content was observed. Water deficit did not alter the pyridine nucleotide concentration but decreased ATP content suggesting metabolic impairment. At a photon flux density of 550 µmol m-2 s-1, the allocation of electrons from photosystem (PS) 2 to O2 reduction was increased by 51 %, while the allocation to CO2 assimilation was diminished by 32 %, as Ψw declined from -0.3 to -2.9 MPa. A significant linear relationship between mean P N and the rate of total linear electron transport was observed in well watered plants, the correlation becoming curvilinear when water deficit increased. The maximum quantum yield of PS2 was not affected by water deficit, whereas qP declined only at very severe stress and the excess photon energy was dissipated by increasing qNP indicating that a greater proportion of the energy was thermally dissipated. This accounted for the apparent down-regulation of PS2 and supported the protective role of qNP against photoinhibition in sunflower.

Keywords: chlorophyll fluorescence; electron transport; fluorescence quenching; Helianthus annuus; intercellular CO2 concentration; net photosynthetic rate; stomatal conductance

Received: July 26, 2007; Accepted: September 20, 2007; Published: March 1, 2008Show citation

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Tezara, W., Driscoll, S., & Lawlor, D.W. (2008). Partitioning of photosynthetic electron flow between CO2 assimilation and O2 reduction in sunflower plants under water deficit. Photosynthetica, 46(1), 127. doi: 10.1007/s11099-008-0020-1.
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    References

    1. Biehler, K., Fock, H.: Evidence for the contribution of the Mehler-peroxidase reaction in dissipating excess electrons in drought-stressed wheat.-Plant Physiol. 112: 265-272, 1996. Go to original source...
    2. Biehler, K., Fock, H.P.: P700 contributes to the dissipation of excessive light energy in water stressed wheat.-Photosynthetica 4: 555-558, 1993.
    3. Björkman, O., Demmig-Adams, B.: Regulation of photosynthetic light energy capture, conversion and dissipation in leaves of higher plants.-In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 17-47. Springer-Verlag, Berlin 1994. Go to original source...
    4. Björkman, O., Powles, S.B.: Inhibition of photosynthetic reactions under water stress: Interaction with light level.-Planta 161: 490-504, 1984. Go to original source...
    5. Boyer, J.S., Wong, S.C., Farquhar, G.D.: CO2 and water vapor exchange across leaf cuticle (epidermis) at various water potentials.-Plant Physiol. 114: 185-191, 1997. Go to original source...
    6. Buckley, T.N., Farquhar, G.D., Mott, KA.: Qualitative effects of patchy stomatal conductance distribution features on gas exchanges calculation.-Plant Cell Environ. 20: 867-880, 1997. Go to original source...
    7. Carrier, J.M., Neve, N.: Oxidation-reduction states of pyridine nucleotides measured by an adapted enzymatic cycling method in maize leaves submitted to anoxia.-Photosynthetica 13: 323-331, 1979.
    8. Centritto, M.: Photosynthetic limitations and carbon partitioning in cherry in response to water deficit and elevated [CO2].-Agr. Ecosyst. Environ. 106: 233-242, 2005. Go to original source...
    9. Centritto, M., Loreto, F., Chartzoulakis, K.: The use of low [CO2] to estimate diffusional and nondiffusional limitations of photosynthetic capacity of salt-stressed olive saplings.-Plant Cell Environ. 26: 585-594, 2003. Go to original source...
    10. Chaves, M.M.: Effects of water deficits on carbon assimilation.-J. exp. Bot. 42: 1-16, 1991. Go to original source...
    11. Cornic, G.: Drought stress and high light effects on leaf photosynthesis.-In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis from Molecular Mechanisms to the Field. Pp. 297-313. Bios Scientific Publ., Oxford 1994.
    12. Cornic, G.: Drought stress inhibits photosynthesis by decreasing stomatal aperture-not by affecting ATP synthesis.-Trends Plant Sci. 5: 187-188, 2000. Go to original source...
    13. Cornic, G., Briantais, J.-M.: Partitioning of photosynthetic electron flow between CO2 and O2 reduction in a C3 leaf (Phaseolus vulgaris L.) at different CO2 concentrations and during drought stress.-Planta 183: 178-184, 1991. Go to original source...
    14. Ehleringer, J., Cook, C.S.: Photosynthesis in Encelia farinosa Gray in response to decreasing leaf water potential.-Plant Physiol. 75: 688-693, 1984. Go to original source...
    15. Epron, D., Dreyer, E.: Effects of severe dehydration on leaf photosynthesis in Quercus petraea (Matt.) Liebl.: photosystem II efficiency, photochemical and non-photochemical fluorescence quenching and electrolyte leakage.-Tree Physiol. 18: 273-284, 1992. Go to original source...
    16. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis.-Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    17. Flexas, J., Bota, J., Escalona, J.M., Sampol, B., Medrano, H.: Effects of drought on photosynthesis in grapevines under field conditions: an evaluation of stomatal and mesophyll limitations.-Funct. Plant Biol. 29: 461-471, 2002. Go to original source...
    18. Flexas, J., Bota, J., Galmés, J., Medrano, H., Ribas-Carbo, M.: Keeping a positive carbon balance under adverse conditions: responses of photosynthesis and respiration to water stress.-Physiol. Plant. 127: 343-352, 2006. Go to original source...
    19. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    20. Habash, D.Z., Paul, M.J., Parry, M.A.J., Keys, A.J., Lawlor, D.W.: Increased capacity for photosynthesis in wheat grown at elevated CO2: the relationship between electron transport and carbon metabolism.-Planta 197: 482-489, 1995. Go to original source...
    21. Havaux, M., Canaani, O., Malkin, S.: Inhibition of photosynthetic activities under slow water stress measured in vivo by the photoacoustic method.-Physiol. Plant. 70: 503-510, 1987. Go to original source...
    22. Heber, U.: Irrungen, Wirrungen? The Mehler reaction in relation to cyclic electron transport in C3 plants.-Photosynth. Res. 73: 223-231, 2002. Go to original source...
    23. Horton, P., Ruban, A., Walters, R.G.: Regulation of light harvesting in green plants. Indication by nonphotochemical quenching of chlorophyll fluorescence.-Plant Physiol. 106: 415-420, 1994. Go to original source...
    24. Jacob, J., Lawlor, D.W.: Stomatal and mesophyll limitations of photosynthesis in phosphate deficient sunflower, maize and wheat plants.-J. exp. Bot. 42: 1003-1011, 1991. Go to original source...
    25. Jacob, J., Lawlor, D.W.: Extreme phosphate deficiency decreases the in vivo CO2/O2 specificity factor of ribulose 1,5-bisphosphate carboxylase-oxygenase in intact leaves of sunflower.-J. exp. Bot. 44: 1635-1641, 1993. Go to original source...
    26. Kitao, M., Lei, T.T., Koike, T., Tobita, H., Maruyama, Y.: Higher electron transport rate observed at low intercellular CO2 concentration in long-term drought-acclimated leaves of Japanese mountain birch (Betula ermanii).-Physiol. Plant. 118: 406-413, 2003. Go to original source...
    27. Krall, J.P., Edwards, G.E.: Relationship between photosystem II activity and CO2 fixation in leaves.-Physiol. Plant. 86: 180-187, 1992. Go to original source...
    28. Krause, G.H., Laasch, H., Weis, E.: Regulation of thermal dissipation of absorbed light energy in chloroplasts indicated by energy dependent fluorescence quenching.-Plant Physiol. Biochem. 26: 445-452, 1988.
    29. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    30. Lawlor, D.W.: The effects of water deficit on photosynthesis.-In: Smirnoff, N. (ed.): Environment and Plant Metabolism. Pp. 129-160. Bios Scientific Publ., Oxford 1995.
    31. Lawlor, D.W., Cornic, G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants.-Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    32. Lawlor, D.W., Khanna-Chopra, R.: Regulation of photosynthesis during water stress.-In: Sybesma, C. (ed.): Advances in Photosynthesis Research. Vol. IV. Pp. 379-382. Martinus Nijhoff/Dr W. Junk Publ., The Hague-Boston-Lancaster 1984. Go to original source...
    33. Loreto, F., Tricoli, D., Di Marco, G.: On the relationship between electron transport rate and photosynthesis in leaves of the C4 plant Sorghum bicolor exposed to water stress, temperature changes and carbon metabolism inhibition.-Aust. J. Plant Physiol. 22: 885-892, 1995. Go to original source...
    34. Osmond, C.B.: Photorespiration and photoinhibition; some implications for the energetics of photosynthesis.-Biochim. biophys. Acta 639: 77-98, 1981. Go to original source...
    35. Peterson, R.B.: Partitioning of noncyclic photosynthetic electron transport to O2-dependent dissipative processes as probed by fluorescence and CO2 exchange.-Plant Physiol. 90: 1322-1328, 1989. Go to original source...
    36. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    37. Quick, W.P., Chaves, M.M., Wendler, R., David, M., Rodrigues, M.L., Passarinho, J.A., Pereira, J.S., Adcock, M.D., Leegood, R.C., Stitt, M.: The effects of water stress on photosynthetic carbon metabolism in four species grown under field conditions.-Plant Cell Environ. 15: 25-35, 1992. Go to original source...
    38. Rackham, O., Wilson, J.: Integrating sphere for spectral measurements on leaves.-In: Wadsworth, R.M. (ed.): The measurement of Environmental Factors in Terrestrial Ecology. Pp. 259-263. Blackwell, London 1968.
    39. Scheuermann, R., Biehler, K., Stuhlfauth, T., Fock, H.P.: Simultaneous gas exchange and fluorescence measurements indicate differences in the response of sunflower, bean and maize to water stress.-Photosynth. Res. 27: 189-197, 1991.
    40. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
    41. Stitt, M., Lilley, R., McCGerhardt, R., Heldt, H.W.: Metabolite levels in specific cells and subcellular compartments of plants leaves.-Methods in Enzymology 174: 518-552, 1989. Go to original source...
    42. Stuhlfauth, T., Beckedahl, J., Fock, H.P.: The response of energy charge, NADPH and free phosphate pools on water stress.-Photosynthetica 25: 11-15, 1991.
    43. Stuhlfauth, T., Scheuermann, R., Fock, H.P.: Light energy dissipation under water stress conditions. Contribution of reasimilation and evidence for additional processes.-Plant Physiol. 92: 1053-1061, 1990. Go to original source...
    44. Stuhlfauth, T., Sültemeyer, D.F., Weinz, S., Fock, H.P.: Fluorescence quenching and gas exchange in a water stressed C3 plant, Digitalis lanata.-Plant Physiol. 86: 246-250, 1988. Go to original source...
    45. Terashima, I., Wong, S.-C., Osmond, C.B., Farquhar, G.D.: Characterisation of non-uniform photosynthesis induced by abscisic acid in leaves having different mesophyll anatomies.-Plant Cell Physiol. 29: 385-394, 1988.
    46. Tezara, W., Martínez, D., Rengifo, E., Herrera, A.: Photosynthetic responses of the tropical spiny shrub Lycium nodosum (Solanaceae) to drought, soil salinity and saline spray.-Ann. Bot. 92: 757-765, 2003. Go to original source...
    47. Tezara, W., Mitchell, V.J., Driscoll, S.P., Lawlor, D.W.: Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP.-Nature 401: 914-917, 1999. Go to original source...
    48. Tezara, W., Mitchell, V., Driscoll, S.P., Lawlor, D.W.: Effects of water deficit and its interaction with CO2 supply on the biochemistry and physiology of photosynthesis in sunflower.-J. exp. Bot. 53: 1781-1791, 2002. Go to original source...
    49. Weis, E., Berry, J.A.: Quantum efficiency of Photosystem II in relation to "energy"-dependent quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 894: 198-208, 1987. Go to original source...

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