Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.
We are always interested to hear from prospective (MSc, PhD or postdoc) candidates. Please submit a short personal statement detailing your research interests and qualifications, and a CV by email, RE: "metabolomics".
GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).
MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.
Projects
White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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Software:
GTTAtlas
MetaboAtlas21
Mapper
Reaction network viewer
Methodological highlighs from publications using small molecule omics techniques.
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN.
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Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects...
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Achievements
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Our laboratory has been awarded the grant GA20-00317S New metabolic pathway in the synthesis of anti-diabetic lipokines (2020-2022).
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Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
Sistilli; Gabriella - Kalendová; Veronika - Čajka; Tomáš - Irodenko; Ilaria - Bardová; Kristina - Oseeva; Marina - Žáček; P. - Kroupová; Petra - Horáková; Olga - Lackner; K. - Gastaldelli; A. - Kuda; Ondřej - Kopecký; Jan - Rossmeisl; Martin
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Krill Oil Supplementation Reduces Exacerbated Hepatic Steatosis Induced by Thermoneutral Housing in Mice with Diet-Induced Obesity
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Nutrients. 2021; 13(2)); 437
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IF = 5.717
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Leal; L. G. - Lopes; Magno Alves - Peres; S. B. - Batista; M. L.; Jr.
Exercise Training as Therapeutic Approach in Cancer Cachexia: A Review of Potential Anti-inflammatory Effect on Muscle Wasting
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Frontiers in physiology. 2021; 11(Feb 4)); 570170
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IF = 4.566
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Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chaves; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C.
Dysregulation of endocannabinoid concentrations in human subcutaneous adipose tissue in obesity and modulation by omega-3 polyunsaturated fatty acids
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Clinical science. 2021; 135(1); 185-200
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IF = 6.124
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Brejchová; Kristýna - Radner; F. P. W. - Balas; L. - Palůchová; Veronika - Čajka; Tomáš - Chodounská; Hana - Kudová; Eva - Schratter; M. - Schreiber; R. - Durand; T. - Zechner; R. - Kuda; Ondřej
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Distinct roles of adipose triglyceride lipase and hormone-sensitive lipase in the catabolism of triacylglycerol estolides
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Proceedings of the National Academy of Sciences of the United States of America. 2021; 118(2)); e2020999118
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IF = 11.205
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Benlebna; M. - Balas; L. - Bonafos; B. - Pessemesse; L. - Fouret; G. - Vigor; C. - Gaillet; S. - Grober; J. - Bernex; F. - Landrier; J.F. - Kuda; Ondřej - Durand; T. - Coudray; Ch. - Casas; F. - Feillet-Coudray; Ch.
Long-term intake of 9-PAHPA or 9-OAHPA modulates favorably the basal metabolism and exerts an insulin sensitizing effect in obesogenic diet-fed mice
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European Journal of Nutrition. 2021; 60(4); 2013-2027
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IF = 5.614
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
