Mgr. Marek Piliarik, Ph.D.

Oblasti vědeckého zájmu:

Nanooptika; biofotonika; mikroskopie; rozptyl světla; Nanočástice; detekce proteinů, jejich struktura a dynamika.

Profesní činnost:

2016 – dnes: Team leader – Nano Optics, Institute of Photonics and Electronics ASCR, v.v.i.  Prague, Czech Republic
2011 – 2016: Postdoctoral Fellow, Max Planck Institute for the Science of Light and Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany
May – July 2011: Postdoctoral Fellow, Swiss Federal Institute of Technology (ETH) in Zürich, Switzerland
2008 – 2011: Postdoctoral Fellow, Institute of Photonics and Electronics ASCR, v.v.i.  Prague, Czech Republic
Sept. 2010: Exchange grant research, Karl-Franzens-University Graz, Institute of Physics, Graz, Austria
2001 – 2002: Visiting Scientist, Electrical Engineering Department, University of Washington, Seattle, USA (11 months)
1998 – 2008: Research Assistant, Institute of Photonics and Electronics ASCR, v.v.i., Prague, Czech Republic (former Institute of Radio Engineering and Electronics)

Vzdělání:

2008: Ph.D. in Quantum optics and optoelectronics
Charles University in Prague, Faculty of Mathematics and Physics

2000: MSc. in Optics and optoelectronics
Charles University in Prague, Faculty of Mathematics and Physics

Fellowships and awards:

2015: Fellowship Jana Evangelisty Purkyně
2011: Max-Planck postdoctoral fellowship.
2010: Exchange Grant fellowship, within the framework of the ESF activity PLASMON-BIONANOSENSE

Bibliometrie:

• 5 kapitol knih
• 35 odborných článků
• >50 příspěvků na konferencích
• 1 patent (+1 čekající)

Vybrané publikace:

1. Ł. Bujak, K. Holanová, A.G. Marin, V. Henrichs, I. Barvík, M. Braun, Z. Lánský, M. Piliarik, Fast leaps between millisecond confinements govern Ase1 diffusion along microtubules, PREPRINT, BioRxiv (2021) 2021.04.15.439939, doi: https://doi.org/10.1101/2021.04.15.439939.
2. Y. He, S. Lin, H. Robert, H. Li, P. Zhang, M. Piliarik, X.-W. Chen, Multiscale Modeling and Analysis for High-fidelity Interferometric Scattering Microscopy, Journal of Physics D: Applied Physics (2021) doi: https://doi.org/10.1088/1361-6463/abf70d.
3. H. Robert, Ł. Bujak, K. Holanová, M. Vala, V. Henrichs, Z. Lánský, M. Piliarik, Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale, Nature Communications 12 (2021) 2921; doi: https://doi.org/10.1038/s41467-021-23252-3.
4. M. Vala, L. Bujak, A.G. Marin, K. Holanova, V. Henrichs, M. Braun, Z. Lansky, M. Piliarik, Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy, Small Methods (2021) 2000985; doi: https://doi.org/10.1002/smtd.202000985.
5. M. Vala, D. Palounek, H. M. L. Robert, M. Piliarik, Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy, Journal of Physics D: Applied Physics (2021); doi: https://doi.org/10.1088/1361-6463/abe669.
6. S. Lin, Y. He, H. Robert, H. Li, P. Zhang, M. Piliarik, X.-W. Chen, Multiscale Modeling and Analysis for High-fidelity Interferometric Scattering Microscopy, PREPRINT, arXiv:2004.10575v3
7. H. Robert, Ł. Bujak, K. Holanová, M. Vala, M. Piliarik Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale, PREPRINT available at Research Square doi: https://doi.org/10.21203/rs.3.rs-38429/v1
8. M.P. McDonald, A. Gemeinhardt, K. König, M. Piliarik, S. Schaffer, S. Völkl, M. Aigner, A. Mackensen, V. Sandoghdar, Visualizing Single-Cell Secretion Dynamics with Single-Protein Sensitivity, Nano Letters  18 (2018) 513.
9. K. Holanová, M. Vala, M. Piliarik, Optical imaging and localization of prospective scattering labels smaller than a single protein, Optics and Laser Technology  109 (2019) 323.
10. S. Spindler, J. Ehrig, K. Koenig, T. Nowak, M. Piliarik, H. Stein, R. Taylor, E. Garanger, S. Lecommandoux, I. Alves, V. Sandoghdar, Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy, Journal of Physics D: Applied Physics 49 (2016) 349601.
11. M. Piliarik, V. Sandoghdar, Direct optical sensing of single unlabeled proteins and super-resolution imaging of their binding sites. Nature Communications 5 (2014) 4495.
    • This publication has been highlighted in:
      • Nature Methods 11 (2014) 990 by Allison Doerr: Imaging without labels
      • Nature Photonics 8 (2014) 674 by Oliver Graydon: Fluorescent-free detection
12. M. Piliarik, J. Homola, Surface plasmon resonance (SPR) sensors: approaching their limits? Optics Express 17 (2009) 16505–16517.
13. H. Vaisocherová, W. Yang, Z. Zhang, Z. Cao, G. Cheng, S. Chen, A. D. Taylor, M. Piliarik, J. Homola, and S. Jiang, Ultra-low Fouling and Functionalizable Surface Chemistry Based on a Zwitterionic Polymer Enabling Sensitive and Specific Protein Detection in Undiluted Blood Plasma, Analytical Chemistry, 80 (2008) 7894–7901. 
14. M. Piliarik, J. Homola , Z. Maníková, J. Čtyroký, Surface Plasmon Resonance Sensor Based on a Single-Mode Polarization-Maintaining Optical Fiber, Sensors and Actuators B 90 (2003) 236-242. 
15. M. Piliarik, H. Vaisocherová and J. Homola, A New Surface Plasmon Resonance Sensor for High-Throughput Screening Applications, Biosensors and Bioelectronics 20 (2005) 2104-2110. 
16. M. Piliarik, L. Párová, and J. Homola, High-Throughput SPR Sensor for Food Safety, Biosensors and Bioelectronics 24 (2009) 1399–1404. 
17. M. Piliarik, M. Vala, I. Tichý, J. Homola, Compact and low-cost biosensor based on novel approach to spectroscopy of surface plasmons, Biosensors and Bioelectronics 24 (2009) 3430-3435. 
18. M. Piliarik, H. Vaisocherová, J. Homola, Surface Plasmon Resonance Biosensing, in Biosensors and Biodetection, Methods in Molecular Biology 503, Ed. A. Rasooly and K. Herold, Humana Press (2009) 65-88. 
19. M. Piliarik, , H. Šípová, P. Kvasnička, N. Galler, J. R. Krenn, J. Homola, High-resolution biosensor based on localized surface plasmons, Optics Express 20 (2012) 672–680. 
20. M. Piliarik, Kvasnička, N. Galler, J. R. Krenn, J. Homola, Local refractive index sensitivity of plasmonic nanoparticles, Optics Express 19 (2011) 9213-9220.

Vybrané přednášky a prezentace:

1. M. Piliarik, High-fidelity fast tracking of protein motion, CPS conference on Wave and quantum aspects of contemporary optics, September 3 - 7 2018 Lednice, Czechia, (Invited talk).
2. M. Piliarik, High-fidelity fast tracking of protein motion, OSA Advanced Photonics Congress, July 2 - 5, 2018, Zurich, Switzerland.
3. M. Piliarik, Single protein detection and single cell analytics, Max-Planck Society – Croucher foundation Joint Workshop on Molecular Systems and Engineering Sciences, April 5 -7, 2016, Hong Kong, China (invited)
4. M. Piliarik, K. König, V. Sandoghdar, Label-free optical detection and super-resolution imaging of single proteins, 18th International Biophysics Congress (IUPAB), August 3-7, 2014, Brisbane, Australia.
5. M. Piliarik, V. Sandoghdar, Label-free single-protein optical biosensing, BIOSENSORS 2012 – 22nd World Congress on Biosensors May 15-18, 2012, Cancun, Mexico. (keynote)
6. M. Piliarik, The future of label-free optical biosensing: from complex matrices to single protein sensitivity, International workshop of Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkers, January 23, 2014, Bratislava. (invited)
7. M. Piliarik, V. Sandoghdar, Label-free single-protein detection, 10th European Conference on Optical Chemical Sensors and Biosensors, Europtrode XI, April 1st- 4th, 2012 Barcelona, Spain.
8. M. Piliarik, Surface plasmon resonance biosensors for multianalyte detection, 2nd Photonics Global conference, December 14-16, 2010, Singapore. (invited)

Patenty:

1. H. Robert, M. Piliarik, Thermo-optical spatial light modulator, Czech patent # 308572, 2020
2. V. Sandoghdar, M. Piliarik, K. König, Method and apparatus for detecting particles, like biological macromolecules or nanoparticles, Pending patent, PCT/EP2015/001804.
3. J. Homola, M. Piliarik, I. Tichý, M. Vala, P. Adam, J. Hepnar, K. Chadt, Surface Plasmon Resonance Coupler and Disperser Sensor, United States of America Patent # 8,094,316, 2012.