Photosynthetica 2019, 57(2):491-499 | DOI: 10.32615/ps.2019.064

Phycobilisome isolation and C-phycocyanin purification from the cyanobacterium Aphanizomenon gracile

R. PIRON1, T. BUSTAMANTE1, A. BARRIGA2, N. LAGOS1
1 Membrane Biochemistry Laboratory, Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Independencia 1027, 8380000 Santiago, Chile
2 Mass Spectrometry Unit, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santos Dumont 964, 8380494 Santiago, Chile

Cyanobacterial phycobilisomes are made up from phycobiliproteins held together by linker proteins. Incident photons are absorbed by the phycobiliproteins that carry linear tetrapyrrole chromophores with extended conjugated bond systems. The chemical structure of the chromophores also bestows the phycobiliproteins with excellent radical scavenging and antioxidant properties and phycobiliproteins have pharmaceutical value as such. We purified and characterized the phycobilisome and its major protein constituent C-phycocyanin from the freshwater filamentous cyanobacterium Aphanizomenon gracile. Isolated phycobilisomes were shown to be intact and contained the phycobiliproteins allophyco-cyanin and C-phycocyanin, but not phycoerythrin, together with the classic set of linker proteins. C-phycocyanin could be easily purified and was of analytical grade with spectral characteristics similar to that of other cyanobacterial species. To our best knowledge, this is the first study to report the purification and characterization of the Aphanizomenon gracile phycobilisome and C-phycocyanin.

Additional key words: mass spectrometry; protein chromatography; protein cross-linking.

Received: August 14, 2018; Accepted: December 5, 2018; Prepublished online: April 16, 2019; Published: May 16, 2019Show citation

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PIRON, R., BUSTAMANTE, T., BARRIGA, A., & LAGOS, N. (2019). Phycobilisome isolation and C-phycocyanin purification from the cyanobacterium Aphanizomenon gracile. Photosynthetica, 57(2), 491-499. doi: 10.32615/ps.2019.064
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    Supplementary files

    Download file2022 MS_data_A-peptide_CHCA+IP.msd

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    Download file2022 MS_data_B-peptide_CHCA+IP.msd

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    Download file2022 MS_data_A-peptide_CHCA.msd

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