Čeština
EnglishUnraveling the chromatin-based mechanisms controlling barley seed development
Nowicka A., Ferkova L., Kovacik M., Said M., Pecinka A
Book of Abstracts - The Czech Plant Nucleus Workshop 2021 - September 14-15 - Olomouc - Czech Republic : , 2021
Klíčová slova:
Abstrakt: Seeds are complex biological structures composed of embryo, endosperm, and protective tissues. Cereal grains represent a special type of seeds, with the largest part formed by the endosperm, a triploid plant-unique tissue nourishing developing embryo. Expansion of some plant organs and/or tissue differentiation is often correlated with local changes in nuclear ploidy. In order to estimate nuclear ploidy dynamics during barley seed development, we measured ploidy of endosperm tissues from pollination until desiccation by flow cytometry. This recognized a phase of active nuclei division followed by endoreduplication and finally selective loss of endoreduplicated nuclei. Microscopic analysis revealed, that endoreduplicated endosperm nuclei had changed morphology, decondensed centromeric heterochromatin, and reduced sister chromatids alignment in an age-dependent manner. This coincided with reduced genome stability, progressive loss of cell viability and finally programmed cell death. Altogether, this project opens new avenues towards understanding nucleus- and chromatin-changes accompanying endoreduplication and programmed cell death phenomena during cereal endosperm development. Funding: This work was supported by the GAČR grants 18-112197S and 21-02929S. https://docs.google.com/forms/u/0/d/e/1FAIpQLSe7DItgQUpQAaz6eiDkgNMV5vj3YuWoIT1wHjB1iXaV8gZsIg/formResponse
DOI:
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Martin Kovačik, Anna Nowicka, Aleš Pečinka, Mahmoud Said
Book of Abstracts - The Czech Plant Nucleus Workshop 2021 - September 14-15 - Olomouc - Czech Republic : , 2021
Klíčová slova:
Abstrakt: Seeds are complex biological structures composed of embryo, endosperm, and protective tissues. Cereal grains represent a special type of seeds, with the largest part formed by the endosperm, a triploid plant-unique tissue nourishing developing embryo. Expansion of some plant organs and/or tissue differentiation is often correlated with local changes in nuclear ploidy. In order to estimate nuclear ploidy dynamics during barley seed development, we measured ploidy of endosperm tissues from pollination until desiccation by flow cytometry. This recognized a phase of active nuclei division followed by endoreduplication and finally selective loss of endoreduplicated nuclei. Microscopic analysis revealed, that endoreduplicated endosperm nuclei had changed morphology, decondensed centromeric heterochromatin, and reduced sister chromatids alignment in an age-dependent manner. This coincided with reduced genome stability, progressive loss of cell viability and finally programmed cell death. Altogether, this project opens new avenues towards understanding nucleus- and chromatin-changes accompanying endoreduplication and programmed cell death phenomena during cereal endosperm development. Funding: This work was supported by the GAČR grants 18-112197S and 21-02929S. https://docs.google.com/forms/u/0/d/e/1FAIpQLSe7DItgQUpQAaz6eiDkgNMV5vj3YuWoIT1wHjB1iXaV8gZsIg/formResponse
DOI:
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Martin Kovačik, Anna Nowicka, Aleš Pečinka, Mahmoud Said