Selection of suitable reference genes for real-time qPCR gene expression in cauliflower under abiotic stress and methyl jasmonate treatmentOriginal Papers

H. LIN, Q.R. ZHANG, J.S. CAO, B.Y. QIU, H.S. ZHU, Q.F. WEN

Biologia plantarum 66:46-55, 2022 | DOI: 10.32615/bp.2021.057  

Cauliflower is one of the most important popular vegetables in China. Cauliflower (Brassica oleracea L. var. botrytis) produces glucosinolates, which are secondary metabolites that have anti-cancer properties. The choice of suitable reference genes (RGs) for gene expression studies has a significant effect on experimental outcomes. In this study, we selected 15 candidates as RGs and analyzed them under heat, cold, drought, and salt stresses and methyl jasmonate (MeJA) treatment. The precision of the real-time quantitative polymerase chain reaction data was assessed using four methods (geNorm, NormFinder, BestKeeper,...

The Czech Plant Nucleus Workshop 2021Conference Report

A. PECINKA, P. PROCHÁZKOVÁ SCHRUMPFOVÁ, L. FISCHER, E. DVOŘÁK TOMAŠTÍKOVÁ, I. MOZGOVÁ

Biologia plantarum 66:39-45, 2022 | DOI: 10.32615/bp.2022.003  

The Czech Plant Nucleus Workshop 2021 (CPNW2021) took place during mid-September 2021 in Olomouc, Czech Republic. About 80 researchers and students working in the field of plant nuclear and chromosome biology in the Czech Republic gathered together to present and discuss their current research. The meeting revealed many plant models that are used to study plant genomes and their organization, and also a great diversity of topics including epigenetic regulation of gene expression, genome stability, telomere biology, or sex chromosomes. CPNW2021 provided a broad platform for establishing new research contacts and collaborations. Here, we summarize the...

Heat stress transcription factor DcHsfA1d isolatedfrom Dianthus caryophyllus enhances thermotoleranceand salt tolerance of transgenic Arabidopsis Original Papers

X.L. WAN, Y. Y. SUN, Y. FENG, M.Z. BAO, J.W. ZHANG

Biologia plantarum 66:29-38, 2022 | DOI: 10.32615/bp.2021.061  

Heat shock transcription factors (Hsfs) participate in a variety of plant physiological processes including the regulation of transcription factors associated with thermotolerance. Here, a Hsf gene DcHsfA1d was identified from carnation (Dianthus caryophyllus L.). The open reading frame (ORF) of DcHsfA1d was 1 368 bp and encoded a protein of 455 amino acids with a molecular mass of 51.039 kDa and an isoelectric point of 4.94. Sequence domain prediction revealed that DcHsfA1d protein exhibited five typical functional features and motifs. The transcription of DcHsfA1d was significantly up-regulated under heat stress...

Variability of leaf pubescence characteristics in transgenic tobacco lines with partial proline dehydrogenase gene suppressionBrief Communications

S.M. IBRAGIMOVA, M.A. GENAEV, A.V. KOCHETOV, D.A. AFONNIKOV

Biologia plantarum 66:24-28, 2022 | DOI: 10.32615/bp.2021.067  

Proline, an amino acid, plays an important role in plants, and it is involved in stress resistance and development. Earlier, to study the proline role in maintaining stress resistance in plants, we obtained genetically modified transgenic lines of tobacco (Nicotiana tabacum L.) with reduced activity of proline dehydrogenase (PDH, the proline degradation gene) and increased content of proline. Transgenic tobacco plants demonstrated greater resistance to high concentrations of NaCl, drought, low temperatures, and heavy metals vs. control plants. The visual assessment showed that the leaf pubescence in transgenic plants varied noticeably....

Identification of TPS and TPP gene families in Cannabis sativa and their expression under abiotic stressesOriginal Papers

J. SUN, Z.G. DAI, X.Y. ZHANG, Q. TANG, C.H. CHENG, C. LIU, Y. YU, G.C. XU, D.W. XIE, J.G. SU

Biologia plantarum 66:14-23, 2022 | DOI: 10.32615/bp.2021.051  

Trehalose is a nonreducing disaccharide that is involved in the regulation of plant responses to a variety of environmental stresses. Trehalose 6-phosphate synthase (TPS) and trehalose 6-phosphate phosphatase (TPP) are two key enzymes in trehalose synthesis and they are widely distributed in higher plants. At present, TPS family genes have been systematically identified and analyzed in many plant species, but the TPP family genes have been rarely studied. In this study, ten TPS and six TPP genes in cannabis (Cannabis sativa L.) were identified at the genomic level. The phylogenetic tree of TPS and TPP...

Identification of key genes related to flowering by transcriptome of flowering and nonflowering Prunella vulgarisOriginal Papers

Y.H. CHEN, Z.Z. LI, Q.S. GUO, C.L. WANG, L.P. CAO, H. TANG, J.Y. HU

Biologia plantarum 66:1-13, 2022 | DOI: 10.32615/bp.2021.056  

Prunella vulgaris L. have high medicinal and ornamental values. It blooms in summer and then quickly withers, and there is no research on the regulatory mechanisms of flowering-related genes. Therefore, in this study, the flowering (leaves and spicas) and nonflowering (leaves) parts of P. vulgaris were sequenced with an Illumina HiSeq 4000, and 187 387 transcripts were obtained using Trinity software package. A total of 10 158 differentially expressed genes (DEGs) were found in the leaves of flowering and nonflowering P. vulgaris, with 6 294 upregulated genes and 3 864 downregulated genes. DEGs in leaves of flowering and...

RETRACTION NOTICE:
Following concerns expressed by a reader, the Editor-in-Chief and Publisher of Biologia Plantarum have taken the decision to retract the following paper. The article is retracted due to concern over image manipulation.
Cucumber PDR8/ABCG36 and PDR12/ABCG40 plasma membrane proteins and their up-regulation under abiotic stresses
BIOLOGIA PLANTARUM 61 (1): 115-126, 2017; DOI: 10.1007/s10535-016-0679-2 Retraction

M. MIGOCKA, A. PAPIERNIAK, A. RAJSZ

Biologia plantarum 65:359, 2021 | DOI: 10.32615/bp.2021.081  

Cloning and functional characterization of a terpene synthase gene AlTPS1 from Atractylodes lancea Original Papers

L.N. CHEN, Y. H. LI, X. HUANG, J. DENG, C. L. QU, X. Q. ZHANG, B.S. HUANG, Y. ZHANG, L. GONG, K. YU

Biologia plantarum 65:351-358, 2021 | DOI: 10.32615/bp.2021.054  

Atractylodes lancea (Thunb.) DC has been used widely as a medicinal herb for centuries and is now being used to treat COVID-19 pneumonia. Terpenoids are thought to be its main pharmacologically active constituents. However, their biosynthesis remains uncharacterized in this species. In this study, the terpene synthase gene AlTPS1 was cloned and functionally characterized. We found that AlTPS1 was a bifunctional enzyme that catalyzed the conversion of farnesyl diphosphate to nerolidol and geranyl diphosphate to linalool in vitro. However, it functioned only in the nerolidol production in vivo by transient expression of the AlTPS1...

Seasonal changes in photosynthesis, phenolic content, antioxidant activity, and anatomy of apical and basal leaves of Aristotelia chilensisOriginal Papers

K. CRISÓSTOMO-AYALA, M. HERNÁNDEZ DE LA TORRE, M.A. PEDREÑO, J.A. HERNÁNDEZ, C. PÉREZ, E. BUSTOS, M. SÁNCHEZ-OLATE, D. RÍOS

Biologia plantarum 65:342-350, 2021 | DOI: 10.32615/bp.2021.052  

Aristotelia chilensis (Mol.) Stuntz is an evergreen species endemic to Chile. It grows in open areas or under tree canopy, and its leaves emerge in early spring and summer. The objective of this study was to determine changes in photosynthetic parameters, total phenol content (TPC), antioxidant activity, and anatomy of apical and basal leaves of A. chilensis during the year. Photosynthesis performance was determined by measuring electron transport rate (ETR), the quantum efficiency of photosystem II (F_v/F_m), photochemical quenching (qP), and non-photochemical quenching (NPQ) with a fluorimeter. Leaf extracts were analysed...

Immunogold-labelling localization of chlorophyllase-2 at different developmental stages of Pachira macrocarpa leavesOriginal Papers

T.C. LEE, K.H. LIN, M.Y. HUANG, C.-M. YANG

Biologia plantarum 65:333-341, 2021 | DOI: 10.32615/bp.2021.048  

Chlorophyllases (Chlases) are housekeeping proteins in plant cells. The dephytylating enzymes can catalyze chlorophyll (Chl) to form chlorophyllide, but the distribution of Chlases in plant cells is still an interesting debate. Previously, we showed that PmCLH2 was a nuclear-encoded gene, and PmCLH2 protein was located in cytosol and chloroplasts of Pachira macrocarpa (Pm). In this study, the antibody of PmCLH2 was made and used by the immunogold-labelling technique to detect the localization of Chlase of Pm leaves at four developmental stages (young, mature, yellowing, and senescent). The transmission electron microscopy...

Potassium silicate combined with glycine betaine improved salt tolerance in Dalbergia odorifera Original Papers

L.-J. ZHANG, E.H.M. CISSE, Y.-J. PU, L.-F. MIAO, L.-S. XIANG, W. XU, F. YANG

Biologia plantarum 65:323-332, 2021 | DOI: 10.32615/bp.2021.044  

Salinity has a huge negative impact on plant growth and development by increasing sodium ions accumulation and potassium ions loss that deeply disturbs the plant cell homeostasis and can lead to plant cell death. The imbalance between Na⁺ and K⁺ could be solved by applying potassium silicate (K₂SiO₃). The glycine betaine (GB) is well-known to play a crucial role against oxidative stress in plants by improving the antioxidant machinery. Thus, this research aimed to apply K₂SiO₃ (1 mM) and GB (10 mM) alone or in combination against 200 mM NaCl-induced damages in Dalbergia odorifera....

Molecular characterization and expression studies of Eucalyptus globulus stress-responsive gene DHN-10Original Papers

G.Z. JAHANGIR, S. NAZ, M.Z. SALEEM, M.I. KHAN, A. YOUNAS, Z. QAMAR, Q. ALI

Biologia plantarum 65:316-322, 2021 | DOI: 10.32615/bp.2019.107  

The Eucalyptus globulus is one of the important forest tree species which reveals enhanced tolerance to multiple abiotic stresses. The enhanced tolerance of E. globulus against low temperature is linked to the presence of enhanced accumulation of lysine-rich dehydrin proteins. The accumulation of dehydrin protein of Mr 10 kDa in response to multiple abiotic stresses prevents cells from dehydration. It is encoded by a novel dehydrin-10 (DHN-10) gene. In the present study, we have used the DHN-10 gene of E. globulus of Pakistani origin, which was cloned in a bacterial expression vector pET30(a) and sequenced. We have...

Melatonin alleviates photoinhibition in cucumber seedlings by modulating partitioning of absorbed excitation energy in photosystem ⅡOriginal Papers

H.L. ZHAO, Y.P. WANG, K. GAO, Y. ZHANG, Y. SHI, Y.X. MIAO

Biologia plantarum 65:307-315, 2021 | DOI: 10.32615/bp.2021.039  

The aim of this study was to evaluate the effects of melatonin on photoinhibition under chilling stress in cucumber seedlings and to inquire into any mechanisms of mitigation. Under chilling stress, the net photosynthetic rate declined dramatically but the decline was significantly mitigated by irrigation with a melatonin solution. Possible mechanisms for this mitigation are that melatonin accelerates xanthophyll de-epoxidation by upregulating the transcription of the violaxanthin de-epoxidase gene (CsVDE) and down-regulating that of the zeaxanthin cyclase gene (CsZE) during chilling. There was also a rise in non-photochemical quenching...

Exogenous paclobutrazol can relieve the low irradiance stress in Capsicum annuum seedlingsOriginal Papers

J.F. HOU, Y. XU, Z.X. WANG, F. CHEN, L.Y. YUAN, S.D. ZHU, G.L. SHAN, C.G. WANG

Biologia plantarum 65:297-306, 2021 | DOI: 10.32615/bp.2021.055  

Irradiance is one of the main limiting factors affecting the production of pepper (Capsicum annuum L.) in facility production. It is therefore important to measure the growth of pepper seedlings under low irradiance and to understand how to relieve low radiation stress. In this study, pepper seedlings were cultivated under low irradiance and were treated with gibberellic acid (GA₃) and paclobutrazol (PP333). Agronomic and physiological characteristics of the pepper seedlings were analyzed. Under low irradiance, the plant height, leaf area, and the chlorophyll (Chl) and malondialdehyde (MDA) content of pepper seedlings were higher...

Identification of three gene families coordinating the conversion between fructose-6-phosphate and fructose-1,6-bisphosphate in wheatOriginal Papers

C.M. YU, Y.C. KE, K.P. ZHANG, M. YAN, H.R. JIN, Y.H. CHEN, J. ZHANG

Biologia plantarum 65:283-296, 2021 | DOI: 10.32615/bp.2021.035  

Saccharides are a direct energy source for most organisms and the primary components in grains of common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD). However, genes involved in the metabolism of primary saccharides such as glucose and fructose have not been fully characterized in wheat, which limits our understanding of how these genes influence wheat growth. In this study, genes coding ATP-dependent phosphofructokinase (PFK), fructose-1,6-bisphosphatase (FBP), and pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP), which participate in the conversion between fructose 6-phosphate (F-6-P) and fructose 1,6-bisphosphate...

Transcriptome analysis of developing castor bean seeds and identification of ricinoleic acid biosynthesis genesOriginal Papers

Z.-T. WU, F. XU, L.-L. YU, Y. OUYANG, X.-X. GENG

Biologia plantarum 65:273-282, 2021 | DOI: 10.32615/bp.2021.033  

Ricinoleic acid is a kind of unsaturated fatty acids in oil of castor bean (Ricinus communis) seeds with wide application value. However, there is little transcriptomic information on genes related to ricinoleic acid biosynthesis in castor beans. To better understand the regulation mechanism of ricinoleic acid biosynthesis, immature seeds at three developmental stages (S1, S2, and S3 corresponding to 15, 30, and 45 d after pollination) were collected. The results indicated that the accumulation of castor bean oil and ricinoleic acid increased gradually during seed development, and reached the maximum value at the late stages of seed development...

Respiration responses of wheat seedlings to treatment with trehalose under heat stressOriginal Papers

Y. LUO, X.-Y. LIU, Y.-J. XUE, X.-Y. CAO, J.-J. LIU, M. GENG

Biologia plantarum 65:265-272, 2021 | DOI: 10.32615/bp.2021.025  

Heat stress limits wheat production and trehalose can improve stress tolerance. How trehalose affects wheat respiration is unclear. In this study, we investigated the effects of exogenous trehalose on the respiration of wheat seedlings during heat stress and the subsequent recovery period. Trehalose pretreatment significantly increased the expression of the alternative oxidase genes AOX1a and AOX1c under heat stress, indicating that trehalose pretreatment increased the capacity of the alternative respiration pathway (AP) in response to heat stress. Trehalose pretreatment also enhanced the activity of the malate-oxaloacetate (Mal-OAA)...

Arabidopsis thaliana AtTFIIB1 gene displays alternative splicing under different abiotic stressesOriginal Papers

J.A. MIRANDA-RÍOS, J.A. RAMÍREZ-TRUJILLO, D.J. JAIME-GALLARDO, N.S. HERNÁNDEZ-BUENO, M. RAMÍREZ-YÁÑEZ, G. ITURRIAGA, R. SUÁREZ-RODRÍGUEZ

Biologia plantarum 65:255-264, 2021 | DOI: 10.32615/bp.2021.022  

In Arabidopsis, there are 14 TFIIB-like proteins that have been phylogenetically categorized into the TFIIB, BRF, and Rrn7/TAF1B/MEE12 subfamilies. The TFIIB transcription factor (TF) subfamily plays a key role in the regulation of gene expression in eukaryotes. To identify the expression patterns of some members of the TFIIB and BRF subfamilies in A. thaliana, different approaches were carried out to determine the possible functions of some of these transcription factors. Through an in silico analysis, we identified possible cis-acting regulatory elements in the promoter regions that drive the expression of transcription...

Methyl jasmonate elicitation of common bean seedlings induces nucleotidase activity and the expression of several nucleotidase genes in radiclesOriginal Papers

G. GALVEZ-VALDIVIESO, M. GARMENDIA-CALVO, M. PINEDA, P. PIEDRAS

Biologia plantarum 65:246-254, 2021 | DOI: 10.32615/bp.2021.017  

Nucleotides are the basic elements of the genetic material, participate in bio-energetic processes, are cofactors and components of secondary metabolites, etc. Nucleotide hydrolases (nucleotidases) are phosphatases that remove the 5'-phosphate group from the nucleotides and play a crucial role in nucleotide metabolism. In this study, genes encoding putative nucleotidases in Phaseolus vulgaris have been identified, and the effect of methyl jasmonate (MeJA) on both nucleotidase activity and gene expression has been addressed. The predicted nucleotidase peptides include the conserved domains characteristic of the haloacid dehalogenase-like...

Obituary - Otília GašparíkováObituary

M. Čiamporová

Biologia plantarum 65:2021  

The complete chloroplast genome of Tamarix ramosissima and comparative analysis of Tamaricaceae speciesOriginal Papers

L. WANG, L. WANG, Z.-H. GUO

Biologia plantarum 65:237-245, 2021 | DOI: 10.32615/bp.2021.014  

Tamarix ramosissima is a deciduous shrub that resides in arid and semi-arid regions. Although of ecological and medicinal values, some Tamarix species are considered invasive as they have dominated the riparian zones of dryland in some parts of the world. Here, the complete chloroplast (cp) genome of T. ramosissima was sequenced and analyzed, showing a size of 156 150 bp and a GC content of 36.5 %. The plastome displayed a typical quadripartite structure, consisting of a pair of inverted repeat (IR) regions of 26 554 bp, separated by a large single copy (LSC) region of 84 795 bp, and a small single copy (SSC) region of 18 247 bp. The...

The effect of Fusarium culmorum inoculation and deoxynivalenol application on proteome response in wheat cultivars Sumai 3 and SW KadriljOriginal Papers

K. KOSOVÁ, J. CHRPOVÁ, J. ŠANTRŮČEK, R. HYNEK, M. KLÍMA, I.T. PRÁŠIL, P. VÍTÁMVÁS

Biologia plantarum 65:221-236, 2021 | DOI: 10.32615/bp.2021.010  

Fusarium head blight (FHB) represents a damaging disease of cereal spikes caused by mycotoxin-producing Fusarium fungi revealing adverse effects on grain quality and yield. Chinese spring wheat (Triticum aestivum L.) cv. Sumai 3 represents a major source of FHB resistance thanks to Fhb1 QTL on 3BS chromosome conferring resistance to FHB and encoding a lectin-like protein. The aim of our study lied in a comparison of proteome response to Fusarium culmorum inoculation and mycotoxin deoxynivalenol (DON) application at 10 d after inoculation (dai) in spikes of resistant wheat cultivar Sumai 3 and susceptible wheat cultivar SW...

Transcriptome comparison of physiological divergence between two ecotypes of Portulaca oleraceaOriginal Papers

M. WU, S. FU, W. JIN, W.Z. XIANG, W.C. ZHANG, L. CHEN

Biologia plantarum 65:212-220, 2021 | DOI: 10.32615/bp.2021.012  

According to the survey, wild purslane (Portulaca oleracea L.) has two different ecotypes, one with the red stem living in an arid environment and one with the green stem living in a humid environment. In order to explore the physiological response strategies of plants to environmental changes, these two ecotypes of purslane were selected as experimental materials. Physiological indices were determined and transcriptome analysis was carried out to screen the differentially expressed genes (DEGs) from two ecotypes of purslane. The results showed that the content of soluble sugars, proline, anthocyanins, and chlorophylls was significantly different,...

Physiological and biochemical changes during graft union formation in Carya illinoinensisOriginal Papers

W.C. SU, H.Y. HE, Z.Z. LIU, Z.H. MO, F. CAO, F.R. PENG

Biologia plantarum 65:203-211, 2021 | DOI: 10.32615/bp.2021.023  

To reveal the physiological and biochemical mechanism underlying graft union formation in pecan (Carya illinoinensis), dynamic changes in content of nutrients, tannin, and phytohormones together with key enzyme activities were investigated 0, 3, 5, 7, 10, 14, 18, 22, 31, and 40 d after grafting (DAG), in homograft unions. During graft union formation, peroxidase (POD) activity increased at 7 - 10 DAG compared with that at 0 DAG. Polyphenol oxidase (PPO) activity was higher in grafted than ungrafted pecan seedlings (control) at 22 DAG, which was similar to POD activity. The tannin content exhibited a decreasing trend with grafting relative to...

Effects of chlorsulfuron and cadmium on metabolites of maize seedlingsOriginal Papers

L. ZHAO, S. YAN, M. WANG, H. ZHANG, X. SHI, J. ZHANG, Y. DENG, L. ZHAO

Biologia plantarum 65:193-202, 2021 | DOI: 10.32615/bp.2021.013  

The impact of persisting herbicide residues on succeeding crops is of great concern to farmers because even the presence of very low concentrations can inhibit growth of crop and cause crop reduction. Furthermore, wastewater irrigation can lead to cadmium accumulation in soils. Thus, the co-occurrence of low amounts of herbicide residues and cadmium within agricultural fields are difficult to avoid. How the combination of these two pollutants affect plant metabolites remains to be elucidated and thus warrants investigation. Maize seeds were planted in soil that had been sprayed with chlorsulfuron and Cd, then we studied the effects of exposure to the...

Spermine alleviates heat-induced senescence in creeping bentgrass by regulating water and oxidative balance, photosynthesis, and heat shock proteinsOriginal Papers

L.L. LIANG, Y.Q. CAO, D. WANG, Y. PENG, Y. ZHANG, Z. LI

Biologia plantarum 65:184-192, 2021 | DOI: 10.32615/bp.2021.008  

Spermine (SPM) is involved in response to abiotic stress in plants, but the potential role of SPM in regulating senescence has not been well documented. Objectives of this study were to examine the effect of changes in endogenous polyamines (PAs) by SPM application on improving heat tolerance of creeping bentgrass (Agrostis stolonifera) and explore the SPM-regulated senescence associated with alterations of water and oxidative balance, photosynthesis, and heat shock proteins under heat stress. The results showed that persistent high temperature caused severe oxidative damage and significant decreases in chlorophyll (Chl) content, photosynthetic...

Purification, immunological, and functional characterization of MxFIT in Malus xiaojinensisOriginal Papers

L.L. YIN, X.L. CHEN, S.L. MA, F. LI, F. ZHOU, Y. WANG

Biologia plantarum 65:177-183, 2021 | DOI: 10.32615/bp.2021.004  

MxFIT is a FER-like iron deficiency induced transcriptional factor in Malus xiaojinensis. Here, we described the heterologous expression of MxFIT in Escherichia coli BL21 (DE3) host cells. The E. coli harboring the recombinant construct pET-MxFIT was efficiently induced to express the MxFIT protein at a high level and the optimal profile for MxFIT expression was investigated. By inoculating a New Zealand rabbit with purified MxFIT-His fusion protein, a high specific anti-MxFIT antiserum was achieved. Western blot analysis showed MxFIT protein expression was induced in roots when iron supply was limiting and was inhibited...

Lysigenous aerenchyma formation: responsiveness to waterlogging in oil palm rootsOriginal Papers

S. NUANLAONG, S. WUTHISUTHIMATHAVEE, P. SURANINPONG

Biologia plantarum 65:167-176, 2021 | DOI: 10.32615/bp.2021.002  

Oil palm (Elaeis guineensis Jacq.) responds to waterlogging stress by induction of lysigenous aerenchyma tissues, which facilitates the flow of oxygen through their root tissues for survival under waterlogged conditions. Thus, the morphological and genetic adaptation involved in lysigenous aerenchyma formation in the roots of the oil palm genotype Deli × Calabar under waterlogging stress was evaluated. This study found the highest number of dead cells after waterlogging stress for 2 d in the secondary root, while the percentage of root porosity was increased with increasing of time in both roots, especially at 1.0 - 2.0 cm from...

Ectopic expression of GmNHX3 and GmNHX1, encoding two Glycine max Na⁺/H⁺ vacuolar antiporters, improves water deficit tolerance in Arabidopsis thalianaOriginal Papers

E.M. PARDO, L. TOUM, L.S. PÉREZ-BORROTO, L. FLEITAS, J.P. GALLINO, S. VIDAL MACHI, A.VOJNOV, A.P. CASTAGNARO, B. WELIN

Biologia plantarum 65:157-166, 2021 | DOI: 10.32615/bp.2021.003  

The importance of Na⁺/H⁺ antiporters in salt tolerance in plants has been demonstrated in many studies, but much less is known about their protective role during drought stress. To study their possible contribution to water deficit tolerance, two closely related soybean Na⁺/H⁺ antiporters belonging to the intracellular NHX exchanger protein family, GmNHX3 and GmNHX1, were evaluated in transgenic Arabidopsis thaliana. A. thaliana plants ectopically expressing GmNHX3 or GmNHX1 displayed a more drought-tolerant phenotype compared to wild-type plants, which was accompanied by an increase...

Physiological and transcriptomic analysis of Pinus massoniana seedling response to osmotic stressOriginal Papers

H. XU, X. GAO, C. YU

Biologia plantarum 65:145-156, 2021 | DOI: 10.32615/bp.2021.016  

Masson pine (Pinus massoniana Lamb.) is an important tree species of high economic value in southern China, but osmotic stress threatens its growth and development. In this study, physiological measurements and RNA-Seq analysis were used to clarify the physiological and molecular responses of P. massoniana under osmotic stress. Osmotic treatment caused cell membrane damage and reactive oxygen species (ROS) accumulation in the tree seedlings, but it also increased their antioxidant enzyme (superoxide dismutase, peroxidase, and catalase) activities and osmotic substances (soluble sugars, proline, and trehalose) content so as to adjust to...