Ústav experimentální medicíny AV ČR, v. v. i.

Illumination

Halogen lamp (HAL 100) for transmission light illumination

Fluorescence light source 100W mercury lamp

Solid-state laser 405nm (35mW)

Ar laser 458nm (25mW)

Ar laser 488nm (25mW)

Ar laser 514nm (25mW)

Solid-state laser 561nm (20mW)

Solid-state laser 633nm (5mW)

Objectives

10X, NA 0.45

20X, NA 0.8

40X, NA 1.2, water

63X, NA 1.4, oil

100X, NA 1.46, oil

Filter sets

CFP; GFP; RFP; YFP; DAPI; Cy5

Detectors

2x confocal PMT detector

1x 32 channel spectral confocal GaAsP detector

1x AiryScan detector

1x Transmitted light detector (T-PMT)

Scanning module

2 galvanometer scanning mirrors

Resolution 512x512 pixels, 10 frames/s

Resolution up to 8000x8000 pixels

Stage

PC-controlled motorized stage

Software

ZEISS ZEN Black Edition

Additional equipment

Temperature and CO₂ controlled incubated enclosure, DIC prism set