Photosynthetica 2019, 57(2):446-457 | DOI: 10.32615/ps.2019.059

Uniconazole confers chilling stress tolerance in soybean (Glycine max L.) by modulating photosynthesis, photoinhibition, and activating oxygen metabolism system

J.J. ZHAO1, N.F. FENG1, X.X. WANG1, G.R. CAI1, M.Y. CAO1, D.F. ZHENG1,2, H.D. ZHU1
1 Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
2 National Coarse Cereals Engineering Research Center, Daqing, Heilongjiang 163000, China

The current study investigates a possible mediatory role of uniconazole in improving chilling stress tolerance in chilling-sensitive (Hefeng 50) and chilling-tolerant (Kenfeng 16) soybean varieties. Chilling stress decreased photosynthetic pigments and inhibited net photosynthetic rate which was ascribed to stomatal limitation. The maximum quantum efficiency of PSII primary photochemistry and electron transport rate were lower in uniconazole-treated plants compared with the control. The increased contents of superoxide (O2*-) and malondialdehyde during chilling stress indicated oxidative stress. Chilling stress reduced glutathione and ascorbic acid contents, and promoted peroxidase, glutathione reductase, and ascorbate peroxidase activities. Uniconazole improved the tolerance of chilled plants. This might be due to better antioxidant defense mechanisms including higher contents of antioxidants and activities of antioxidant enzymes, which retard lipid peroxidation. Thus, uniconazole has a positive effect and improves the chilling resistance of soybean.

Additional key words: carotenoid; catalase; chlorophyll; reactive oxygen species; superoxide dismutase.

Received: January 4, 2018; Accepted: October 15, 2018; Prepublished online: April 4, 2019; Published: May 16, 2019Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
ZHAO, J.J., FENG, N.F., WANG, X.X., CAI, G.R., CAO, M.Y., ZHENG, D.F., & ZHU, H.D. (2019). Uniconazole confers chilling stress tolerance in soybean (Glycine max L.) by modulating photosynthesis, photoinhibition, and activating oxygen metabolism system. Photosynthetica, 57(2), 446-457. doi: 10.32615/ps.2019.059
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Allen D.J., Ort D.R.: Impacts of chilling temperatures on photosynthesis in warm-climate plants. - Trends Plant Sci. 6: 36-42, 2001. Go to original source...
    2. Al-Rumaih M.M., Al-Rumaih M.M.: Physiological response of two species of datura to uniconazole and salt stress. - J. Food Agric. Environ. 46: 663-668, 2015.
    3. Arnon D.T.: Copper enzymes in isolated chloroplasts. Polyphe-noloxidase in Beta vulgaris. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    4. Aro E.M., Virgin I., Andersson B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover. - Biochim. Biophys. Acta 1143: 113-134, 1993. Go to original source...
    5. Balestrasse K.B., Tomaro M.L., Batlle A., Noriega, G.O.: The role of 5-aminolevulinic acid in the response to cold stress in soybean plants. - Phytochemistry 71: 2038-2045, 2010. Go to original source...
    6. Cakmak I., Marschner H.: Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. - Plant Physiol. 98: 1222-1227, 1992. Go to original source...
    7. Chen F., Wang F., Wu F. et al.: Modulation of exogenous glutathione in antioxidant defense system against Cd stress in the two barley genotypes differing in Cd tolerance. - Plant Physiol. Bioch. 48: 663-672, 2010. Go to original source...
    8. Chucheep K., Kanlayanarat S., Matsuo T.: Analogous effect of preheating on chilling sensitivity of mung bean seedlings and membrane viscosity to effects of ABA and uniconazole treatments. - J. Food Agric. Environ. 3: 110-116, 2005.
    9. Demmig B., Björkman O.: Comparison of the effect of excessive light on chlorophyll fluorescence (77 K) and photon yield of O2 evolution in leaves of higher plants. - Planta 171: 171-184, 1987. Go to original source...
    10. Dhindsa R.S., Plumb-Dhindsa P., Thorpe T.A.: Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. - J. Exp. Bot. 32: 93-101, 1981. Go to original source...
    11. Duan L., Guan C., Li J. et al.: Compensative effects of chemical regulation with uniconazole on physiological damages caused by water deficiency during the grain filling stage of wheat. - J. Agron. Crop Sci. 194: 9-14, 2008. Go to original source...
    12. Egli D.B.: Flowering, pod set and reproductive success in soya bean. - J. Agron. Crop Sci. 191: 283-291, 2005. Go to original source...
    13. Erdal S.: Androsterone-induced molecular and physiological changes in maize seedlings in response to chilling stress. - Plant Physiol. Bioch. 57: 1-7, 2012. Go to original source...
    14. Esterbauer H., Grill D.: Seasonal variation of glutathione and glutathione reductase in needles of Picea abies. - Plant Physiol. 61: 119-121, 1978. Go to original source...
    15. Fu J., Huang B.: Involvement of antioxidants and lipid peroxi-dation in the adaptation of two cool-season grasses to localized drought stress. - Environ. Exp. Bot. 45: 105-114, 2001. Go to original source...
    16. Funatsuki H., Matsuba S., Kawaguchi K. et al.: Methods for evaluation of soybean chilling tolerance at the reproductive stage under artificial climatic conditions. - Plant Breeding 123: 558-563, 2004. Go to original source...
    17. Gill S.S., Tuteja N.: Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. - Plant Physiol. Bioch. 48: 909-930, 2010. Go to original source...
    18. Grace S.C., Logan B.A.: Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. - Plant Physiol. 112: 1631-1640, 1996. Go to original source...
    19. Hall A.E.: Crop Response to Environment. Pp. 248. CRC Press, New York 2000. Go to original source...
    20. Hasanuzzaman M., Hossain M.A., Teixeira da Silva J.A., Fujita M.: Plant response and tolerance to abiotic oxidative stress: Antioxidant defense is a key factor. - In: Venkateswarlu B., Shanker A., Shanker C., Maheswari M. (ed.): Crop Stress and its Management: Perspectives and Strategies. Pp. 261-315. Springer, Dordrecht 2012. Go to original source...
    21. Ikeda T., Ohnishi S., Senda M. et al.: A novel major quantitative trait locus controlling seed development at low temperature in soybean (Glycine max). - Theor. Appl. Genet. 118: 1477-1488, 2009. Go to original source...
    22. He J., Lin L., Ma Q. et al.: Uniconazole (S-3307) strengthens the growth and cadmium accumulation of accumulator plant Malachium aquaticum. - Int. J. Phytoremediat. 19: 348-352, 2017. Go to original source...
    23. Kreslavski V.D., Los D.A., Allakhverdiev S.I., Kuznetsov V.V.: Signaling role of reactive oxygen species in plants under stress. - Russ. J. Plant. Physl+ 59: 141-154, 2012. Go to original source...
    24. Leul M., Zhou W.: Alleviation of waterlogging damage in winter rape by application of uniconazole: Effects on morphological characteristics, hormones and photosynthesis. - Field Crop. Res. 59:121-127, 1998. Go to original source...
    25. Liu Y., Jiang H., Zhao Z., An L.: Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana. - J. Plant Physiol. 168: 853-862, 2011. Go to original source...
    26. Liu Y., Zhao Z., Si J. et al.: Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana. - Plant Growth Regul. 59: 207-214, 2009. Go to original source...
    27. Liu Y.F., Qi M.F., Li T.L.: Photosynthesis, photoinhibition, and antioxidant system in tomato leaves stressed by low night temperature and their subsequent recovery. - Plant Sci. 196: 8-17, 2012. Go to original source...
    28. Liu Y.F., Zhang G.X., Qi M.F., Li T.L.: Effects of calcium on photosynthesis, antioxidant system, and chloroplast ultrastructure in tomato leaves under low night temperature stress. - J. Plant Growth Regul. 34: 263-273, 2015. Go to original source...
    29. Manafi E., Modarres Sanavy S.A.M., Aghaalikhani M., Dolatabadian A.: Exogenous 5-aminolevulenic acid promotes antioxidative defence system, photosynthesis and growth in soybean against cold stress. - Not. Sci. Biol. 7: 486-494, 2015. Go to original source...
    30. Nahar K., Hasanuzzaman M., Alam M.M., Fujita M.: Exogenous spermidine alleviates low temperature injury in mung bean (Vigna radiata L.) seedlings by modulating ascorbate-glutathione and glyoxalase pathway. - Int. J. Mol. Sci. 16: 30117-30132, 2015. Go to original source...
    31. Nahar K., Hasanuzzaman M., Alam M.M. et al.: Polyamine and nitric oxide crosstalk: antagonistic effects on cadmium toxicity in mung bean plants through upregulating the metal detoxification, antioxidant defense, and methylglyoxal detoxification systems. - Ecotox. Environ. Safe. 126: 245-255, 2016. Go to original source...
    32. Ögren E.: Prediction of photoinhibition of photosynthesis from measurements of fluorescence quenching components. - Planta 184: 538-544, 1991. Go to original source...
    33. Ohnishi S., Miyoshi T., Shirai S.: Low temperature stress at different flower developmental stages affects pollen development, pollination, and pod set in soybean. - Environ. Exp. Bot. 69: 56-62, 2010. Go to original source...
    34. Polle A., Otter T., Seifert F.: Apoplastic peroxidases and lignifi-cation in needles of Norway (Picea abies L.). - Plant Physiol. 106: 53-60, 1994. Go to original source...
    35. Qiu J., Wang R., Yan J., Hu J.: Seed film coating with uniconazole improves rape seedling growth in relation to physiological changes under waterlogging stress. - Plant Growth Regul. 47: 75-81, 2005. Go to original source...
    36. Saito S., Okamoto M., Shinoda S. et al.: A plant growth retardant, uniconazole, is a potent inhibitor of ABA catabolism in Arabidopsis. - Biosci. Biotech. Bioch.70: 1731-1739, 2006. Go to original source...
    37. Savitch L.V., Gray G.R., Huner N.P.A.: Feedback-limited photo-synthesis and regulation of sucrose-starch accumulation during cold acclimation and low-temperature stress in a spring and winter wheat. - Planta 201: 18-26, 1997. Go to original source...
    38. Singh S.K., Kakani V.G., Surabhi G.K., Reddy K.R.: Cowpea (Vigna unguiculata [L.] Walp.) genotypes response to multiple abiotic stresses. - J. Photoch. Photobio. B 100: 135-146, 2010. Go to original source...
    39. Strauss A.J., Krüger G.H.J., Strasser R.J., Van Heerden P.D.R.: Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P. - Environ. Exp. Bot. 56: 147-157, 2006. Go to original source...
    40. Takahashi R., Benitez E.R., Funatsuki H., Ohnishi S.: Soybean maturity and pubescence color genes improve chilling tolerance. - Crop Sci. 45: 1387-1393, 2005. Go to original source...
    41. Tian X., Liu Y., Huang Z. et al.: Comparative proteomic analysis of seedling leaves of cold-tolerant and -sensitive spring soybean cultivars. - Mol. Biol. Rep. 42: 581-601, 2015. Go to original source...
    42. Todoroki Y., Kobayashi K., Shirakura M. et al.: Abscinazole-F1, a conformationally restricted analogue of the plant growth retardant uniconazole and an inhibitor of ABA 8'-hydroxylase CYP707A with no growth-retardant effect. - Bioorgan. Med. Chem. 17: 6620-6630, 2009. Go to original source...
    43. Upadhyaya A., Davis T.D., Larsen M.H. et al.: Uniconazole-induced thermotolerance in soybean seedling root tissue. - Physiol. Plantarum 79: 78-84, 1990. Go to original source...
    44. Wan Z., Kothare M.V.: Uniconazole-induced tolerance of rape plants to heat stress in relation to changes in hormonal levels, enzyme activities and lipid peroxidation. - Plant Growth Regul. 27: 99-104, 1999.
    45. Wang A.G., Luo G.H.: Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. - Plant Physiol. Commun. 6: 55-57, 1990.
    46. Wei W., Li Q.T., Chu Y.N. et al.: Melatonin enhances plant growth and abiotic stress tolerance in soybean plants. - J. Exp. Bot. 66: 695-707, 2015. Go to original source...
    47. Yan Y., Wan Y., Liu W. et al.: Influence of seed treatment with uniconazole powder on soybean growth, photosynthesis, dry matter accumulation after flowering and yield in relay strip intercropping system. - Plant Prod. Sci. 18: 295-301, 2015. Go to original source...
    48. Zhang J., Kirkham M.B.: Antioxidant responses to drought in sunflower and sorghum seedlings. - New Phytol. 132: 361-373, 1996. Go to original source...
    49. Zhang M., Duan L., Tian X. et al.: Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system. - J. Plant Physiol. 164: 709-717, 2007. Go to original source...
    50. Zhao H., Ye L., Wang Y. et al.: Melatonin increases the chilling tolerance of chloroplast in cucumber seedlings by regulating photosynthetic electron flux and the ascorbate-glutathione cycle. - Front. Plant Sci. 7: 1814, 2016. Go to original source...

    Return to the content