Photosynthetica, 2008 (vol. 46), issue 1

Photosynthetica 2008, 46(1):73-78 | DOI: 10.1007/s11099-008-0013-0

Mutation mechanism of chlorophyll-less barley mutant NYB

Z. L. Liu1, S. Yuan1, W. J. Liu1, J. B. Du1, W. J. Tian1, M. H. Luo1, H. H. Lin1,*
1 Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Science, Sichuan University, Chengdu, Sichuan, P. R. China

NYB is chlorophyll-less barley mutant, which is controlled by a recessive nuclear gene. The mutation mechanism is revealed. The activities of enzymes transforming 5-aminolevulinic acid into protochlorophyllide were the same in both NYB and the wild type (WT), but the activity of the protochlorophyllide oxidoreductase (POR) in WT was much higher than that of NYB. Most of the photosystem 2 apoproteins were present in both WT and NYB, suggesting that the capability of protein synthesis was probably fully preserved in the mutant. Thus chlorophyll (Chl) biosynthesis in NYB was hampered at conversion form protochlorophyllide (Pchlide) into chlorophyllide. The open reading frame of porB gene in NYB was inserted with a 95 bp fragment, which included a stop codon. The NYB mutant is a very useful material for studies of Chl biosynthesis, chloroplast signalling, and structure of light-harvesting POR-Pchlide complex (LHPP).

Keywords: chlorophyll; fluorescence emission spectra; light-harvesting complex 2; Nanchong yellow barley (NYB); nucleotide sequences; photosystem 2; proteins; protochlorophyllide oxidoreductase (POR)

Received: March 20, 2007; Accepted: September 3, 2007; Published: March 1, 2008Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Liu, Z.L., Yuan, S., Liu, W.J., Du, J.B., Tian, W.J., Luo, M.H., & Lin, H.H. (2008). Mutation mechanism of chlorophyll-less barley mutant NYB. Photosynthetica, 46(1), 73-78. doi: 10.1007/s11099-008-0013-0.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Anderson, J.M., Waldron, J.C., Thorne, S.W.: Chlorophyll protein complexes of spinach and barley thylakoids. Spectral characterization of six complexes resolved by an improved electrophoretic procedure.-FEBS Lett. 92: 227-233, 1978. Go to original source...
    2. Bogorad, L.: Porphyrin synthesis.-Methods Enzymol. 5: 885-891, 1962. Go to original source...
    3. Cheng, Y., Tang, Z.S., Wang, Z.X., Yang, F.: Research of physiology and heredity on barley of chlorophyll-deficient mutant.-J. Sichuan Teachers Coll. (nat. Sci.) 22: 131-133, 2001.
    4. Dei, M.: Benzyladenine-induced stimulation of 5-aminolevulinic acid accumulation under various light intensities in levulinic acid-treated cotyledons of etiolated cucumber.-Physiol. Plant. 64: 153-160, 1985. Go to original source...
    5. Eggink, L.L., Park, H., Hoober, J.K.: The role of chlorophyll b in photosynthesis: Hypothesis.-BMC Plant Biol. 1(2): http://www.biomedcentral.com/1471-2229/1/2, 2001.
    6. Falbel, T.G., Meehl, J.B., Staehelin, L.A.: Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis.-Plant Physiol. 112: 821-832, 1996. Go to original source...
    7. Hoober, J.K., Eggink, L.L.: Assembly of light-harvesting complex II and biogenesis of thylakoid membranes in chloroplasts.-Photosynth. Res. 61: 197-215, 1999. Go to original source...
    8. Krawetz, S.A., Pon, R.T., Dixon, G.H.: Increased efficiency of the Taq polymerase catalyzed polymerase chain reaction.-Nucleic Acids Res. 17: 819, 1989. Go to original source...
    9. Kropat, J., Oster, U., Rudiger, W., Beck, C.F.: Chloroplast signaling in the light induction of nuclear HSP70 genes requires the accumulation of chlorophyll precursors and their accessibility to cytoplasm/nucleus.-Plant J. 24: 523-531, 2000. Go to original source...
    10. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    11. Lay, P.L., Eullaffroy, P., Juneau, P., Popovic, R.: Evidence of chlorophyll synthesis pathway alteration in desiccated barley leaves.-Plant Cell Physiol. 41: 565-570, 2000. Go to original source...
    12. Lichtenthaler, H.K., Wellburn, A.R.: Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591-592, 1983. Go to original source...
    13. Lin, H.H., He, J.X., Liang, H.G., Du, L.F.: Studies on light harvesting complexes of photosystem II in a chlorophyll-deficient barley mutant NYB.-Acta biochim. biophys. sin. 30: 530-532, 1998.
    14. Lin, H.H., He, J.X., Liang, H.G., Du, L.F.: Qualitative and quantitative differences in the light harvesting complexes of photosystem II (LHC II) between a chlorophyll-less barley mutant and its wild type.-J. Plant Physiol. 154: 152-156, 1999. Go to original source...
    15. Lin, Z.-F., Peng, C.-L., Lin, G.-Z., Zhang, J.-L.: Alteration of components of chlorophyll-protein complexes and distribution of excitation energy between the two photosystems in two new rice chlorophyll b-less mutants.-Photosynthetica 41: 589-595, 2003. Go to original source...
    16. Masuda, T., Takamiya, K.: Novel insights into the enzymology, regulation and physiological functions of light-dependent protochlorophyllide oxidoreductase in angiosperms.-Photosynth. Res. 81: 1-29, 2004. Go to original source...
    17. Mohanty, S., Grimm, B., Tripathy, B.C.: Light and dark modulation of chlorophyll biosynthetic genes in response to temperature.-Planta 224: 692-699, 2006. Go to original source...
    18. Nott, A., Jung, H.-S., Koussevitzky, S., Chory, J.: Plastid-to-nucleus retrograde signalling.-Annu. Rev. Plant Biol. 57: 739-759, 2006. Go to original source...
    19. Pfannschmidt, T.: Chloroplast redox signals: how photosynthesis controls its own genes.-Trends Plant Sci. 8: 33-41, 2003. Go to original source...
    20. Rebeiz, C.A., Mattheis, J.R., Smith, B.B., Rebeiz, C.C., Dayton, D.F.: Chloroplast biogenesis. Biosynthesis and accumulation of protochlorophyll by isolated etioplasts and developing chloroplasts.-Arch. Biochem. Biophys. 171: 549-567, 1975. Go to original source...
    21. Reinbothe, C., Buhr, F., Bartsch, S., Desvignes, C., Quigley, F., Pesey, H., Reinbothe, S.: In vitro-mutagenesis of NADPH: protochlorophyllide oxidoreductase B: two distinctive protochlorophyllide binding sites participate in enzyme catalysis and assembly.-Mol. gen. Genomics 275: 540-552, 2006. Go to original source...
    22. Reinbothe, C., Lebedev, N., Reinbothe, S.: A protochlorophyllide light-harvesting complex involved in de-etiolation of higher plants.-Nature 397: 80-84, 1999. Go to original source...
    23. Reinbothe, C., Pollmann, S., Desvignes, C., Weigele, M., Beck, E., Reinbothe, S.: LHPP, the light-harvesting NADPH:protochlorophyllide (Pchlide) oxidoreductase:Pchlide complex of etiolated plants, is developmentally expressed across the barley leaf gradient.-Plant Sci. 167: 1027-1041, 2004. Go to original source...
    24. Rudoi, A.B., Shcherbakov, R.A.: Analysis of the chlorophyll biosynthetic system in a chlorophyll b-less barley mutant.-Photosynth. Res. 58: 71-80, 1998. Go to original source...
    25. Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning. A Laboratory Manual. 2nd Ed.-Cold Spring Harbor Laboratory, Cold Spring Harbor 1989.
    26. Strand, A., Asami, T., Alonso, J., Ecker, J.R., Chory, J.: Chloroplast to nucleus communication triggered by accumulation of Mg-protoporphyrin IX.-Nature 421: 79-83, 2003. Go to original source...
    27. Tan, X.X., Xu, D.Q., Tang, Z.S.: A cause of higher photochemical efficiency of photosystem II in a chlorophyll-deficient barley mutant.-Acta phytophysiol. sin. 23: 251-256, 1997.
    28. Tang, Z.S., Tang, Y.: The Golden Champaign of Nangchong Yellow Barley.-Sichuan Science & Technology Press, Chengdu 1994.
    29. Wettstein, D. von, Gough, S., Kannangara, C.G.: Chlorophyll biosynthesis.-Plant Cell 7: 1039-1057, 1995. Go to original source...
    30. Yuan, S., Liu, Z.L., Liu, W.J., Lei, T., Luo, M.H., Du, J.B., Wang, J.H., Lin, H.H.: A chlorophyll-less barley mutant "NYB" is insensitive to water stress.-Z. Naturforsch. 62c: 403-409, 2007. Go to original source...
    31. Zhang, N.H., Wei, Z.Q., He, J.X., Du, L.F., Liang, H.G.: An efficient and economic method for preparation of high quality plant RNA.-Progr. Biochem. Biophys. 31: 947-950, 2004.

    Return to the content