Čeština
EnglishThe impact of increased soil risk elements on carotenoid contents
Procházková D., Haisel D., Pavlíková D., Száková J., Wilhelmová N.
CENTRAL EUROPEAN JOURNAL OF BIOLOGY 9: 678-685, 2014
Keywords: Arsenic, cadmium, zinc, xanthophyll cycle, neoxanthin, lutein
Abstract: A pot experiment was conducted to compare a response of non-transgenic tobacco plant (WT) and those with genetically prolonged life-span (SAG) to risk elements. Plants were grown in control soil and in soil with increased levels of As, Cd and Zn. The pigment contents were established by HPLC and chlorophyll fluorescence parameters were measured from slow kinetics after a 15 min dark period with the PAM fluorometer. Top (i.e. young) leaves of both WT and SAG plants were more sensitive to photoinhibition caused by the harmful elements but plants showed acclimation to such elements in bottom leaves. Plants differed in the participation of individual pigments of xanthophyll cycle: increased levels of risk elements seem to stimulate especially first (violaxanthin to antheraxanthin) and second (anhtheraxanthin to zeaxanthin) steps of the cycle in WT plants. In SAG plants, toxic elements caused an increase in the content, particularly of the initial compound of the cycle - violaxanthin.
DOI:
IEB authors: Daniel Haisel, Naďa Wilhelmová
CENTRAL EUROPEAN JOURNAL OF BIOLOGY 9: 678-685, 2014
Keywords: Arsenic, cadmium, zinc, xanthophyll cycle, neoxanthin, lutein
Abstract: A pot experiment was conducted to compare a response of non-transgenic tobacco plant (WT) and those with genetically prolonged life-span (SAG) to risk elements. Plants were grown in control soil and in soil with increased levels of As, Cd and Zn. The pigment contents were established by HPLC and chlorophyll fluorescence parameters were measured from slow kinetics after a 15 min dark period with the PAM fluorometer. Top (i.e. young) leaves of both WT and SAG plants were more sensitive to photoinhibition caused by the harmful elements but plants showed acclimation to such elements in bottom leaves. Plants differed in the participation of individual pigments of xanthophyll cycle: increased levels of risk elements seem to stimulate especially first (violaxanthin to antheraxanthin) and second (anhtheraxanthin to zeaxanthin) steps of the cycle in WT plants. In SAG plants, toxic elements caused an increase in the content, particularly of the initial compound of the cycle - violaxanthin.
DOI: