Hoyerová K., Perry L., Hand P., Laňková M., Kocábek T., May S., Kottová J., Pačes J., Napier R., Zažímalová E.
PLANT PHYSIOLOGY 146: 1128–1141, 2008

Klíčová slova: Plant hormones, polar auxin transport, auxin influx carrier, AUX1, Arabidopsis, tobacco
Abstrakt: We have isolated the cDNA of the gene PaLAX1 from a wild cherry tree (Prunus avium). The gene and its product are highly similar in sequences to both the cDNAs and the corresponding protein products of AUX/LAX-type genes, coding for putative auxin influx carriers. We have prepared and characterized transformed tobacco (Nicotiana tabacum) and Arabidopsis (Arabidopsis thaliana) plants carrying the gene PaLAX1. We have proved that constitutive overexpression of PaLAX1 is accompanied by changes in the content and distribution of free indole-3-acetic acid, the major endogenous auxin. The increase in free indole-3-acetic acid content in transgenic plants resulted in various phenotype changes, typical for the ‘auxin-overproducing’ plants. The uptake of synthetic auxin, 2,4-dichlorophenoxyacetic acid, was three times higher in transgenic lines compared to the wild type lines and the treatment with the auxin uptake inhibitor, 1-naphthoxyacetic acid, reverted the changes caused by the expression of PaLAX1. Moreover, the agravitropic response could be restored by expression of PaLAX1 in the mutant aux1 plants, which are deficient in auxin influx carrier activity. Based on our data, we have concluded that the product of the gene PaLAX1 promotes the uptake of auxin into cells and, as a putative auxin influx carrier, it affects the content and distribution of free endogenous auxin in transgenic plants.
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Autoři z ÚEB: Klára Hoyerová, Martina Laňková, Eva Zažímalová