Biologia plantarum, 1970 (vol. 12), issue 5

Article

Content of free amino acids and their exosmose from maize kernels in relation to cold resistance

V. Segeťa, Eliška Vedralová

Biologia plantarum 12:315-326, 1970 | DOI: 10.1007/BF02922185

Maize caryopses were exposed in moist, sterile sand to temperatures of 24°, 10° and 6°. The amino acid content of embryo, endosperm and pericarp tissues of kernels and amino acid occurence in sand eluates was analysed by paper chromatography.The lower the temperature to which the seeds were exposed and the greater the delay in start of germination, the greater were the amounts of amino acids exosmosed from the kernel to the surrounding media. The exudation of aspartic acid, proline, alanine, γ-aminobutyric and glutamic acids was especially high.The time-course of amino acid content in various kernel parts proves that cold does not check...

Electron microscopic autoradiographic study of RNA isolated from apple-tree callus tissue labelled with 6-Benzylaminopurine-8-C¹⁴

Alena Volfová, A. Friedrich, L. Chvojka

Biologia plantarum 12:327-331, 1970 | DOI: 10.1007/BF02922186

In the present paper RNA from apple-tree callus tissue labelled with 6-benzyl-minopurine¹⁴) was studied. The RNA isolated from this tissue was prepared as sample for electron microscopic studies and was also used as biochemical control. The electron microscopic autoradiograms obtained showed the labelled structure of RNA, sRNA and rRNA. The incorporation of labelled purine rings was confirmed in all three RNA types by the radioactivity, which was also proved in nucleotides after hydrolysation of RNA fractions. The results were compared with RNA isolated from tissue cultivated on a non-radiactive medium.

Use of acid, alkali and enzymes for the extraction of nucleic acids from pollen

J. Süss

Biologia plantarum 12:332-341, 1970 | DOI: 10.1007/BF02922187

Various methods for extracting nucleic acids from pollen were tested to find a suitable procedure for obtaining a pure preparation of nucleic acids uncontaminated by polysaccharides and polyphosphates without the use of ion exchangers. Extraction was carried out with perchloric acid, potassium hydroxide, ribonuclease and deoxyribonuclease, sodium tetraborate, and combinations of these. In all fractions, residues of precipitates and residues of extracted pollen matter, the quantity of RNA, DNA, proteins and concomitants,i.e. Polysaccharides and polyphosphates, was determined. The purity of preparations was checked by means of UV-spectra. The...

Transport, localization and physiological effect of 6-benzyladenme-8-¹⁴C in apple shoots

A. Friedrich, L. Chvojka, R. Bulgakov, J. Kolín

Biologia plantarum 12:342-347, 1970 | DOI: 10.1007/BF02922188

The transport of radioactivity of 6-benzyladenine-8-¹⁴C applied to one-year old apple shoots was studied. Simultaneously, labelled metabolites of this cytokinin were studied separately in the xylem and phloem above and below the place of application. According to the results obtained, it can be assumed that 6-B and its metabolites are transported in one-year old apple shoots acropetally through the xylem and basipetally through the phloem, while penetrating from the xylem to the phloem. Besides 6-B-8-¹⁴C, a complex of cytokinin with sugar, also adenosine and adenine were found in the phloem.

Effect of β-indoleacetic acid, maleic hydrazide, and 2,3,5-triiodobenzoic acid on N, P, K, and Ca accumulation by pea plants

Alena Raková, J. Minar

Biologia plantarum 12:348-358, 1970 | DOI: 10.1007/BF02922189

A study was performed on the effect of various concentrations of IAA, 2,3,6-triiodobenzoic acid, and maleic hydrazide, supplied to Richter's nutrient solution, on growth of pea plants in water cultures. After a 18-day cultivation growth was evaluated and in the plants gathered the content of total N, P, K, and Ca was estimated.Growth of experimental plants (as evaluated from fresh and dry weight) was affected by all three regulators in dependence on the concentration used. It was stimulated by lower concentrations and inhibited by higher, the production of both fresh and dry weight of the root system being stimulated by all IAA concentrations used.The...

The growth rate variability of Chlorella vulgaris beijer. Clones grown after mutagen treatment under autotrophic or heterotrophic conditions on the surface of a solid medium

J. Nečas

Biologia plantarum 12:359-367, 1970 | DOI: 10.1007/BF02922190

Populations of theChloretta vulgaris Beijeb. strain Česnokov "V" clones (progenies of single cells) grown up from cells treated by chemical mutagens were cultivated on the surface of solid media. The differentiation of growth types according to their growth rates in the populations originating from the same sample of cells but growing under different conditions was compared. The strain studied exhibited much greater growth rate variability on the complete medium than on the minimal one. Short time changes of light regime during the cultivation for the purpose of inducing reparation processes in the mutagen damaged cells did not manifest themselves...