Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.
GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).
MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.
Projects
White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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Software:
GTTAtlas
MetaboAtlas21
Mapper
Reaction network viewer
Methodological highlighs from publications using small molecule omics techniques.
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Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects...
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN.
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Achievements
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Our laboratory has been awarded the grant GA20-00317S New metabolic pathway in the synthesis of anti-diabetic lipokines (2020-2022).
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Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
Štěpán; M. - Daďová; K. - Matouš; M. - Krauzová; E. - Sontáková; L. - Koc; M. - Larsen; T. - Kuda; Ondřej - Štich; V. - Rossmeislová; L. - Šiklová; M.
Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women
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Nutrients. 2022; 14(1)); 149
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IF = 6.706
[ASEP]
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Riečan; Martin - Palůchová; Veronika - Lopes; Magno - Brejchová; Kristýna - Kuda; Ondřej
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Branched and linear fatty acid esters of hydroxy fatty acids (FAHFA) relevant to human health
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Pharmacology and Therapeutics. 2022; 231(Mar)); 107972
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IF = 13.4
[ASEP]
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doi
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Oeckl; J. - Janovská; Petra - Adamcová; Kateřina - Bardová; Kristina - Brunner; S. - Dieckmann; S. - Ecker; J. - Fromme; T. - Funda; Jiří - Gantert; T. - Giansanti; P. - Soledad Hidrobo; M. - Kuda; Ondřej - Kuster; B. - Li; Y. - Pohl; Radek - Schmitt; S. - Schweizer; S. - Zischka; H. - Zouhar; Petr - Kopecký; Jan - Klingenspor; M.
Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat
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Molecular Metabolism. 2022; 61(July)); 101499
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IF = 8.568
[ASEP]
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doi
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Fisk; H. L. - Childs; C. E. - Miles; E. A. - Ayres; R. - Noakes; P. S. - Paras-Chavez; C. - Kuda; Ondřej - Kopecký; Jan - Antoun; E. - Lillycrop; K. A. - Calder; P. C.
Modification of subcutaneous white adipose tissue inflammation by omega-3 fatty acids is limited in human obesity-a double blind; randomised clinical trial
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EBioMedicine. 2022; 77(Mar)); 103909
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IF = 11.205
[ASEP]
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doi
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Brejchová; Kristýna - Palůchová; Veronika - Březinová; Marie - Čajka; Tomáš - Balas; L. - Durand; T. - Křížová; M. - Straňák; Z. - Kuda; Ondřej
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Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase
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Food Chemistry. 2022; 388(Sep 15)); 132983
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IF = 9.231
[ASEP]
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
