Čeština
EnglishHormonal responses associated with acclimation to freezing stress in Lolium perenne
Přerostová, S., Zupková, B., Petřík, I., Šimura, J, Našinec, I., Kopecký, D., Knirsch, V., Gaudinová, A., Novák, O., Vaňková, R.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY 182: 104295, 2021
Keywords: cold acclimation, cold stress, freezing stress, Lolium perenne, phytohormone, RT-qPCR
Abstract: Complex phytohormonal analyses revealed hormone changes underlying the freezing response of perennial ryegrass (Lolium perenne L.). Comparisons among sensitive (code of accession 1/61), highly tolerant (5/101) and semi-tolerant (6/104; sensitive without acclimation, but tolerant after acclimation) genotypes allowed identification of changes associated with freezing tolerance. The main response to freezing stress (- 7 ◦C for four days) was a significant elevation of abscisic acid (ABA) in all tissues of all genotypes, supported by elevation of jasmonic acid (JA) in leaves, and of salicylic acid (SA) in crowns and roots. Jasmonate-isoleucine increase was observed in roots of the tolerant genotype. The auxin (indole-3-acetic acid) levels showed relatively minor changes, while cytokinins exhibited up-regulation in crowns of sensitive genotypes. Tolerant genotypes exhibited decreased levels of highly active cytokinin trans-zeatin compensated by elevated low-active cis-zeatin, involved in stress responses. Cold acclimation (3 ◦C for seven days) applied prior frost treatment was associated with stimulation of plant defences, especially expression of ice recrystallisation inhibition protein LpIRI3. Acclimation diminished the impact of subsequent freezing treatment as indicated by lower up-regulation of stress hormones ABA, JA and SA in all tested tissues (with the exception of SA in crowns and roots of sensitive genotype) and upregulation of active cytokinins in tolerant and semi-tolerant genotypes, especially in crowns and roots.
DOI: 10.1016/j.envexpbot.2020.104295
Fulltext: contact IEB authors
IEB authors: David Kopecky
ENVIRONMENTAL AND EXPERIMENTAL BOTANY 182: 104295, 2021
Keywords: cold acclimation, cold stress, freezing stress, Lolium perenne, phytohormone, RT-qPCR
Abstract: Complex phytohormonal analyses revealed hormone changes underlying the freezing response of perennial ryegrass (Lolium perenne L.). Comparisons among sensitive (code of accession 1/61), highly tolerant (5/101) and semi-tolerant (6/104; sensitive without acclimation, but tolerant after acclimation) genotypes allowed identification of changes associated with freezing tolerance. The main response to freezing stress (- 7 ◦C for four days) was a significant elevation of abscisic acid (ABA) in all tissues of all genotypes, supported by elevation of jasmonic acid (JA) in leaves, and of salicylic acid (SA) in crowns and roots. Jasmonate-isoleucine increase was observed in roots of the tolerant genotype. The auxin (indole-3-acetic acid) levels showed relatively minor changes, while cytokinins exhibited up-regulation in crowns of sensitive genotypes. Tolerant genotypes exhibited decreased levels of highly active cytokinin trans-zeatin compensated by elevated low-active cis-zeatin, involved in stress responses. Cold acclimation (3 ◦C for seven days) applied prior frost treatment was associated with stimulation of plant defences, especially expression of ice recrystallisation inhibition protein LpIRI3. Acclimation diminished the impact of subsequent freezing treatment as indicated by lower up-regulation of stress hormones ABA, JA and SA in all tested tissues (with the exception of SA in crowns and roots of sensitive genotype) and upregulation of active cytokinins in tolerant and semi-tolerant genotypes, especially in crowns and roots.
DOI: 10.1016/j.envexpbot.2020.104295
Fulltext: contact IEB authors
IEB authors: David Kopecky