2.2. 2023, Metabolismus bioaktivních lipidů

Laboratory name: Metabolism of Bioactive Lipids

Laboratory website

PhD project: Human breast milk – the unique underexplored liquid

Nourishment plays a crucial role in the early stage of life. Breast milk provides the ideal cocktail of nutrients for infants in this period of rapid development and growth, and breastfeeding has a long-term impact on health. Lifestyle changes and the higher age of mothers result in an increased incidence of preterm birth, gestational diabetes mellitus (GDM) and other complications in perinatal time period. However, the effects on milk lipidome and metabolome are poorly explored. The aim of the project is to perform the pilot study to explore the potential of 2H2O to label milk lipids in mice and women; to perform the observational study and explore the effect of GDM on milk lipidome and de novo lipogenesis; and to test whether dietary regime or anti-diabetic medication yield the same milk metabolome in GDM and healthy mothers.

References:

Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase. Brejchova K, Paluchova V, Brezinova M, Cajka T, Balas L, Durand T, Krizova M, Stranak Z, Kuda O. Food Chem. 2022 Sep 15;388:132983. https://doi.org/10.1016/j.foodchem.2022.132983

Levels of palmitic acid ester of hydroxystearic acid (PAHSA) are reduced in the breast milk of obese mothers. Brezinova M, Kuda O, Hansikova J, Rombaldova M, Balas L, Bardova K, Durand T, Rossmeisl M, Cerna M, Stranak Z, Kopecky J. Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Feb;1863(2):126-131. https://doi.org/10.1016/j.bbalip.2017.11.004

Metabolomics atlas of oral 13C-glucose tolerance test in mice. Lopes M, Brejchova K, Riecan M, Novakova M, Rossmeisl M, Cajka T, Kuda O. Cell Rep. 2021 Oct 12;37(2):109833. https://doi.org/10.1016/j.celrep.2021.109833

Lipokine 5-PAHSA Is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for De Novo Lipogenesis in Mice. Paluchova V, Oseeva M, Brezinova M, Cajka T, Bardova K, Adamcova K, Zacek P, Brejchova K, Balas L, Chodounska H, Kudova E, Schreiber R, Zechner R, Durand T, Rossmeisl M, Abumrad NA, Kopecky J, Kuda O. Diabetes. 2020 Mar;69(3):300-312. https://doi.org/10.2337/db19-0494

Supervisor (email): Ondrej Kuda, Ph.D. ondrej.kuda@fgu.cas.cz

Candidate’s profile (requirements): We are seeking outstanding self-motivated candidates with master's degree or equivalent in biochemistry, analytical chemistry, molecular biology, physiology, medicine or related fields, or those expecting to obtain their degree this year. Candidates should be fluent in English. Experience with in vivo models (mouse) as well as with in vitro cell cultures and omics techniques are advantage.