Ústav experimentální medicíny AV ČR, v. v. i.

Illumination

Halogen lamp (HAL 100) for transmission light illumination

Fluorescence light source 100W mercury lamp

Solid-state laser 405nm (35mW)

Ar laser 458nm (25mW)

Ar laser 488nm (25mW)

Ar laser 514nm (25mW)

Solid-state laser 561nm (20mW)

Solid-state laser 633nm (5mW)

Objectives

10X, NA 0.45

20X, NA 0.8

40X, NA 1.2, water immersion, correction collar

63X, NA 1.4, oil immersion

100X, NA 1.46, oil immersion

Filter sets

CFP; GFP; RFP; YFP; DAPI; Cy5

Detectors

2x confocal PMT detector

1x 32 channel spectral confocal GaAsP detector

1x AiryScan detector

1x Transmitted light detector (T-PMT)

Scanning module

2 galvanometer scanning mirrors

Resolution 512x512 pixels, 10 frames/s

Resolution up to 8000x8000 pixels

Stage

PC-controlled motorized stage

Software

ZEISS ZEN Black Edition

Additional equipment

Temperature and CO₂ controlled incubated enclosure, DIC prism set