2.2. 2023, Laboratory of Metabolism of Bioactive Lipids

Laboratory name: Metabolism of Bioactive Lipids

Laboratory website

PhD project: New metabolomics dimensions - subcellular and inter-organ communication

Cells communicate with each other via three main channels: juxtracrine, paracrine, and endocrine signaling. The spatiotemporal resolution represents an unexplored territory that could reveal a new layer of communication or regulation. Advances in various techniques allow us to explore communication across scales (from organelles to organs) and add new dimensions to our understanding of human diseases. The aim of the project is to develop chemical tags for subcellular metabolomics, to apply metabolic tracers labeled with stable isotopes at the level of organelles and the whole organism, and to combine them in mouse models of type 2 diabetes.

References:

Metabolomics atlas of oral 13C-glucose tolerance test in mice. Lopes M, Brejchova K, Riecan M, Novakova M, Rossmeisl M, Cajka T, Kuda O. Cell Rep. 2021 Oct 12;37(2):109833. https://doi.org/10.1016/j.celrep.2021.109833

Lipokine 5-PAHSA Is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for De Novo Lipogenesis in Mice. Paluchova V, Oseeva M, Brezinova M, Cajka T, Bardova K, Adamcova K, Zacek P, Brejchova K, Balas L, Chodounska H, Kudova E, Schreiber R, Zechner R, Durand T, Rossmeisl M, Abumrad NA, Kopecky J, Kuda O. Diabetes. 2020 Mar;69(3):300-312. https://doi.org/10.2337/db19-0494

The role of peroxiredoxin 6 in biosynthesis of FAHFAs. Paluchova V, Cajka T, Durand T, Vigor C, Dodia C, Chatterjee S, Fisher AB, Kuda O. Free Radic Biol Med. 2022 Nov 20;193(Pt 2):787-794. https://doi.org/10.1016/j.freeradbiomed.2022.11.015

Triacylglycerols containing branched palmitic acid ester of hydroxystearic acid (PAHSA) are present in the breast milk and hydrolyzed by carboxyl ester lipase. Brejchova K, Paluchova V, Brezinova M, Cajka T, Balas L, Durand T, Krizova M, Stranak Z, Kuda O. Food Chem. 2022 Sep 15;388:132983. https://doi.org/10.1016/j.foodchem.2022.132983

Supervisor (email): Ondrej Kuda, Ph.D. ondrej.kuda@fgu.cas.cz

Candidate’s profile (requirements): We are seeking outstanding self-motivated candidates with master's degree or equivalent in biochemistry, analytical chemistry, molecular biology, physiology, medicine or related fields, or those expecting to obtain their degree this year. Candidates should be fluent in English. Experience with in vivo models (mouse) as well as with in vitro cell cultures and omics techniques are advantage.