Analysis of Bacteriophage–Host Interaction by Raman Tweezers
- Zdeněk Pilát*Zdeněk Pilát*Email: [email protected]Institute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Zdeněk Pilát,
- Alexandr JonášAlexandr JonášInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Alexandr Jonáš,
- Jana PilátováJana PilátováDepartment of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 128 44 Prague 2, Czech RepublicMore by Jana Pilátová,
- Tereza KlementováTereza KlementováInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Tereza Klementová,
- Silvie BernatováSilvie BernatováInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Silvie Bernatová,
- Martin ŠilerMartin ŠilerInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Martin Šiler,
- Tadeáš MaňkaTadeáš MaňkaInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Tadeáš Maňka,
- Martin KizovskýMartin KizovskýInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Martin Kizovský,
- Filip RůžičkaFilip RůžičkaDepartment of Microbiology, Faculty of Medicine, Masaryk University and St. Anne’s Faculty Hospital, Pekařská 53, 656 91 Brno, Czech RepublicMore by Filip Růžička,
- Roman PantůčekRoman PantůčekDepartment of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech RepublicMore by Roman Pantůček,
- Ute NeugebauerUte NeugebauerCenter for Sepsis Control and Care (CSCC), Jena University Hospital, Am Klinikum 1, D-07747 Jena, GermanyLeibniz Institute of Photonic Technology (Leibniz-IPHT), Albert-Einstein-Str. 9, D-07745 Jena, GermanyMore by Ute Neugebauer,
- Ota SamekOta SamekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Ota Samek, and
- Pavel ZemánekPavel ZemánekInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Czech Academy of Sciences, Královopolská 147, 612 64 Brno, Czech RepublicMore by Pavel Zemánek
Abstract
Bacteriophages, or “phages” for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage–host interaction on a molecular level. We employed Raman tweezers to analyze the phage–host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80α of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage–host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
Cited By
This article is cited by 1 publications.
- S. Bernatová, K. Rebrošová, Z. Pilát, M. Šerý, A. Gjevik, O. Samek, J. Ježek, M. Šiler, M. Kizovský, T. Klementová, V. Holá, F. Růžička, P. Zemánek. Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers. The European Physical Journal Plus 2021, 136 (2) https://doi.org/10.1140/epjp/s13360-021-01152-1