LaVision UltraMicroscope II

Upright fluorescence microscope with light-sheet technology for visualization of whole biological samples.
Microscope is dedicated to project Neurorecon.

Application

Fast 3D imaging of whole cleared biological samples
Multi-channel, Z-stack, positions, mosaic
Suitbale for common clearing media (Cubic, uDIsco)

Affiliation

Centrum rekonstrukčních neurověd

Výzkumné centrum

Hlavní vedoucí

James Fawcett

Location

Lb building, 0.19A

Downloads

CellSens manual
Stáhnout PDF
ScanR Acquisition manual
Stáhnout PDF
ScanR Analysis manual
Stáhnout PDF

Specification

Illumination

Up to 6 light sheets
Solid-state laser 488 nm (50 – 100 mW)
Solid-state laser 561 nm (50 – 100 mW)
Solid-state laser 640 nm (50 – 100 mW)

Objectives

Olympus MVPLAPO 2x, NA 0.5, wd 10 mm.
Additional manual zoom (post-objective) 0.63x – 63x

Filter sets

525/50 (green)
620/60 (red)
680/30 (far-red)

Detectors

sCMOS camera Andor Ixon
5.5 megapixel
Pixel size 6.5×6.5 um
Frame rate up to100 fps

Refractive index matching

1.33 – 1.56

Stage

PC-controlled motorized chamber

Software

Inspector control software, Imaris

Additional equipment

Contact

Mgr. Barbora Hyklová, Ph.D.

+420 241 062 718

barbora.hyklova@iem.cas.cz

Zobrazit více

Light-Sheet mikroskop LaVision UltraMicroscope II