Electrochemical analysis of proteins important in cancer: Behavior of anterior gradient receptors, p53 protein and their complexes at charged surfaces

Abstract

Electrochemical methods are one of the most utilized approaches for newly developing strategies of cancer biomarker detection. However, electrochemical methods can be used in cancer research also as techniques applicable for fast preliminary tests of protein structural changes and for clarifying protein interactions with the interacting partners including DNA, proteins, and peptides. In this review, we present some examples of electrochemical approaches, which help to understand the role of cancer protein(s) in biological pathways. Data obtained with protein p53 and anterior gradient proteins using chronopotentiometric stripping analysis provide information about the structural dynamics of a given protein and its complexes. The knowledge about biomacromolecule behavior at the charged surfaces can be further utilized in other fields including molecular biology, proteomics, glycomics as well as in sensorics.