The department is focused on the mechanisms of toxic effects of engineered nanoparticles as well as combustion originated fine and ultrafine particles. Major attention is paid to the interaction of particles and their components with biomolecules in human cell lines. Currently, DGTE is focused on the role of ultrafine particles in the etiology of neurodegenerative diseases. The methods include various tests of cytotoxicity, the detection of reactive oxygen species and oxidative damage of DNA, proteins and lipids (ELISA), analyzes of genotoxicity (Comet assay, DNA adducts) and clastogenic effects (micronucleus test and cytogenetic analysis by FISH), as well as analyzes of protein expression (western blotting) and DNA repair activity (NER and NHEJ). To get more insight into the mechanism of biological effects of particles or their extracts, the toxicogenomic methods (microarrays and next generation sequencing) and RT-PCR are used. Most of these tests are also available to external users from research, universities, industry and academia via the Research Infrastructure NanoEnviCz (www.nanoenvicz.cz).
Head of the Department
Res. Prof. Jan Topinka, Ph.D.
Maternal Diet Quality and the Health Status of Newborns
The study evaluated the quality of the maternal diet and the health status of newborns. The results showed insufficient intake of vegetables, dairy products and fiber. The intake of the most persistent organic pollutants did not exceed limits, only the intake of perfluorinated substances exceeded the limit for 7.8% of women. Birth weight had a positive relationship with protein intake. Neonatal oxidative stress had a positive relationship with DDT intake and a negative relationship with protein intake.
Graphical abstract of the study and its results. First, the nutritional quality of the maternal diet was evaluated from the detailed food records (1) and chemical analysis of food samples contamination by persistent organic pollutants was performed (2). Subsequently, the relationships of these two factors with birth weight (3) and neonatal oxidative stress (4) were studied.
Publication:
Pavlíková, J., Ambrož, A., Hoňková, K., Chvojková, I., Šrám, R.J., Rössner, P., Topinka, J., Gramblička, T., Parizek, O., Parizkova, D., Pulcmanová, J., Rössnerová, A.: (2022) Maternal Diet Quality and Health Status of Newborns. Foods. 11(23): 3893.
Individual DNA Methylation Pattern Shifts in Nanoparticle-Exposed Workers Analyzed in Four Consecutive Years
Our study focused on the impact of nanoparticle exposure on DNA methylation. We aimed to map methylation changes over time at the individual level for 4 years. The results showed fluctuation in methylation settings in both exposed and control persons. Overall, the changes were more pronounced in the exposed subjects, although for several CpG sites no modification was noted. These sites are linked with the process of adaptation, which is fixed by the process of the epigenetic memory.
Individual DNA methylation pattern trajectories. Examples of individual DNA methylation pattern trajectories of the nanoparticles exposed (#1 – #10) and control subjects (#11 – #14) analyzed by principal component analysis in four consecutive years (2016 – 2019): results of the whole CpG data set analyzed by 850K array chips.
Publication:
Rössnerová, A., Hoňková, K., Chvojková, I., Pelcová, D., Ždímal, V., Hubáček, J. A., Lischková, L., Vlčková, Š., Ondráček, J., Dvořáčková, Š., Topinka, J., Rössner, P.: (2021) Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years. International Journal of Molecular Sciences. 22(15): 7834. PMID: 34360600, PMCID: PMC8346047, doi: 10.3390/ijms22157834 (IF = 5.924).
Projects
1. 5. 2019 – 30. 4. 2023
Transport derived Ultrafines and the Brain Effects
Department of Genetic Toxicology and EpigeneticsPřichystalová R.Caron-Beaudoin E.Richardson l.Dirkx E.Amadou A.Závodná T.Cihak R.Cogliano V.Hynes J.Pelland-St-Pierre L.Verner M.A.van Tongeren M.Ho V.
2021
Journal of Exposure Science and Environmental Epidemiology. 07/20.
Department of Genetic Toxicology and EpigeneticsMussalo L.Avesani S.Shahbaz M. A.Závodná T.Saveleva L.Järvinen A.Lampinen R.Belaya I.Krejčík Z.Ivanova M.Hakkarainen H.Kalapudas J.Penttilä E.Löppönen H.Koivisto A. M.Malm T.Topinka J.Giugno R.Aakko-Saksa P.Chew S.Rönkkö T.Jalava P.Kanninen K. M.
2023
Sci Total Environ . 2023 Sep 13;905:167038. doi: 10.1016/j.scitotenv.2023.167038. Online ahead of print.
Department of Genetic Toxicology and EpigeneticsJandacka D.Uchytil J.Zahradnik D.Farana R.Vilimek D.Skypala J.Urbaczka J.Plesek J.Motyka A.Blaschova D.Beinhauerova G.Rygelova M.Brtva P.Balazova K.Horka V.Malus J.Freedman Silvernail J.Irwin G.Nieminen M.T.Casula V.Juras V.Golian M.Elavsky S.Knapova L.Šrám R.Hamill J.
2020
International Journal of Environmental Research and Public Health. 17(23): 9142.
Department of Genetic Toxicology and EpigeneticsMartikainen M.V.Aakko-Saksa P.van den Broek L.Flemming R.C.Carare R.O.Chew S.Dinnyes A.Giugno R.Kanninen K.M.Malm T.Muala A.Nedergaard M.Oudin A.Oyola P.Pfeiffer T.V.Ronkko T.Sandstrom T.Schins R.P.F.Topinka J.Yang M.Zeng X.Westerink R.H.S.Jalava P.I.
2022
International Journal of Environmental Research and Public Health. 19(1): 311.