Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes and LIMeX platform.
GTTAtlas - interactive metabolomics and lipidomics atlas of mouse tissues during oral glucose tolerance test (OGTT).
MetaboAtlas21 - a comprehensive atlas of the mouse metabolome and lipidome.
LORA - a Lipid Over-Representation Analysis tool.
Projects
Software:
GTTAtlas
MetaboAtlas21
Mapper
Reaction network viewer
LORA
Methodological highlighs from publications using small molecule omics techniques.
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White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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Naturally occurring long-chain omega-3 PUFA, namely eicosapentaenoic acid (EPA; 20:5 ω-3), docosapentaenoic acid (DPA; 22:5 ω-3), and docosahexaenoic acid (DHA; 22:6 ω-3) exert multiple biological effects...
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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We run local instances of several services focused on Metabolomics, Lipidomics, Statistical analysis and Chemometrics. As the hardware resources are limited, these tools are available only within campus LAN.
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Achievements
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Our laboratory has been awarded the grant GA20-00317S New metabolic pathway in the synthesis of anti-diabetic lipokines (2020-2022).
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Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
Vondráčková; Michaela - Kopczynski; D. - Hoffmann; N. - Kuda; Ondřej
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LORA; Lipid Over-Representation Analysis Based on Structural Information
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Analytical Chemistry. 2023; 95(34); 12600-12604
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IF = 7.4
[ASEP]
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doi
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Song; J. - Duivenvoorde; L. P. M. - Grefte; S. - Kuda; Ondřej - Martínez-Ramírez; Felipe - van der Stelt; I. - Mastorakou; Dimitra - van Schothorst; E. M. - Keijer; J.
Normobaric hypoxia shows enhanced FOXO1 signaling in obese mouse gastrocnemius muscle linked to metabolism and muscle structure and neuromuscular innervation
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Pflugers Archiv - European Journal of Physiology. 2023; 475(11); 1265-1281
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IF = 4.5
[ASEP]
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doi
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Martínez-Ramírez; Felipe - Riečan; Martin - Čajka; Tomáš - Kuda; Ondřej
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Analysis of fatty acid esters of hydroxy fatty acids in edible mushrooms
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LWT-Food Science and Technology. 2023; 173(1 Jan)); 114311
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IF = 6.0
[ASEP]
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doi
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Hricko; Jiří - Rudl Kulhavá; Lucie - Paučová; Michaela - Nováková; Michaela - Kuda; Ondřej - Fiehn; O. - Čajka; Tomáš
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Short-Term Stability of Serum and Liver Extracts for Untargeted Metabolomics and Lipidomics
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Antioxidants. 2023; 12(5)); 986
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IF = 7.0
[ASEP]
[
doi
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Drastichová; Z. - Trubačová; Radka - Novotný; J.
Regulation of phosphosignaling pathways involved in transcription of cell cycle target genes by TRH receptor activation in GH1 cells
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Biomedicine & Pharmacotherapy. 2023; 168(Dec); 115830
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IF = 7.5
[ASEP]
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doi
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
