Equipment | Year | Dept | Location | Use for | Manager/ Operator | ||
---|---|---|---|---|---|---|---|
FemtoJet® 4i (programmable microinjector) + InjectMan® 4 (micromanipulator) | 2023 | 80 | DA1 001 | microinjection of adherent cells | Markéta Hlaváčková | ||
Bruker Ultima IntraVital | 2018 | 60 | DAI | upright multiphoton microscope for in vivo and in vitro imaging |
Daniel Hadraba / David Vondrášek |
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Leica SP8 AOBS WLL MP confocal | 2015 | 60 | DAI 10 | Inverted laser scanning and multiphoton microscope |
Daniel Hadraba/ David Vondrášek |
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Leica SPE confocal | 2007 | 60 | DAI 9 | Upright laser scanning and fluorescence microscope |
Daniel Hadraba/ David Vondrášek |
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Optical projection tomography | 2011 | 60 | DAI 2 | 3D imaging of transparent samples | Radochová Barbora | ||
CellVizio Optic fibre confocal microscopy | 2015 | 33 | D 050 | Optic fibre confocal microscope capable of fluorescence imaging from surface to deep tissues in whole animal. See more. | Jakub Otáhal / David Kala | ||
Neuroimage - stereology microscope | 2010 | 33 | D 048 | Neuroimage (MBF Bioscience) | Hana Kubová | ||
Leica LMD 6000 Laser capture microdissector | 2008 | 21 | D 239 | Allows to identify and dissect microareas from tissue or cell smear. | Martin Vodička | ||
Carv II system | 2013 | 32 | D 048 | Spinning disc confocal microscope |
Daniel Hadraba / David Vondrášek |
Equipment | Year | Dept | Location | Use for | Manager/Operator | |
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Qtrap 5500 mass spectrometer | 2012 | 17 | E 218 | metabolomics, lipidomics |
Ondřej Kuda/ Ivana Brabcová |
Equipment | Year | Dept | Location | Use for | Manager | ||
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Behavioral analysis |
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PhenoWorld - automatic modular system | 2015 | 32 | G | Modular system for complex behavioral analyses | Petr Ergang | ||
Multi conditioning system | 2011 | 32 | D 037 | System accommodating nine standard conditioning paradigms for the evaluation of learning, memory, emotion and stress-related behaviors in mice and rats. | Štěpán Kubík | ||
Phenotyper - system for behavioral analysis | 2013 | 33 | D 011 | PhenoTyper (Noldus) for 8 rats. System is designed for automatated behavioral analysis in instrumented observation cage. | Hana Kubová | ||
Hematology Analyzer scil Vet abc Plus+ | 2016 | 41 | E 017 | Analytical Instrument to determine haematological parameters from whole blood | Světlana. Žufanová | ||
CT/PET |
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microCT/PET Albira | 2013 | 96 | E 013 | In vivo imaging of small laboratory animals | Roman Liška | ||
Echocardiography |
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GE-VIVID 7 | 2010 | 80 | D 325 | Echocardiographic assessment of rat heart geometry and function | František Papoušek | ||
VisualSonics Vevo 3100 | 2022 | 80 | D 302b | Echocardiographic assessment of mouse heart geometry and function | František Papoušek | ||
Blood pressure |
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Telemetric monitoring of blood pressure | 2013 | 25 | D | Long term telemetric monitoring of blood pressure and sympathetic activity | Ivana Vaněčková | ||
Metabolism |
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Indirect calorimetry INCA Somedic | 2012 | 17 | G 008 | Indirect calorimetry for mice and rats | Kristína Bardová |
Equipment | Year | Dept | Location | Use for | Manager | |
---|---|---|---|---|---|---|
Oroboros Oxygraph 2k | 2013 | 13+17 | DAII 108 | Three setups for measurement of cellular/mitochondrial respiration. One with fluorescent attachment for ROS/ATP monitoring | Petr Pecina | |
Seahorse Xfe-24 alanyzer | 2014 | 13 | DAII 228 | Plate based system for measurement of respiration and glycolytic activity of cells | Tomáš Mráček |
Equipment | Year | Dept | Location | Use for | Manager | |
---|---|---|---|---|---|---|
2017 | 80 | D3 335 | incubation and handling of cells under hypoxic conditions | Markéta Hlaváčková | ||
Nanodrop spectrophotometer | 2007 | 17 | D 224 | Measurement of nucleic acid concentration and purity and fluorescent dye labeled proteins. | Karla Vágnerová | |
Light Cycler 480 real-time PCR | 2009 | 17 | D 224 | Real-time PCR system | Veronika Kleinová | |
ViiA 7 real-time PCR | 2012 | 17 | D 224 | Real-time PCR system with HRM possibility | Karla Vágnerová | |
Labchip GX Touch | 17 | D 237 |
Nucleic Acid Analyzer- automated capillary electrophoresis |
Karla Vágnerová | ||
Nanodrop One c | 17 | D 237 | Measurement of nuclei acids and proteins concentration | Karla Vágnerová | ||
Chemidoc | 17 | D 237 | Imaging system for the fluorescence and chemiluminescence detection | Karla Vágnerová | ||
QX200 Droplet Digital PCR System | 2020 | 33 | D 237/238 | System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications | Šárka Růžičková | |
Osmometer OSMOMAT 3000 Basic | 2020 | 24 | D 215 | osmolarity measurement (buffers, media) | Martin Sládek | |
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Orbital Shaker/Incubator | 1997 | 24 | D 2xx (corridor) | bacteria growth (up to 3L flasks) | Martin Sládek |
Equipment |
Year |
Dept |
Location |
Use for |
Manager |
|
---|---|---|---|---|---|---|
Microbeta counter | 2019 | 14 | D 121 | scintillation counting in a plate format | Jan Jakubík | |
Reader Tecan infinity M200 | 2007 | 13 | DAII 112 | Luminescence, Fluorescence (top reading), UV-absorbance (UV-VIS) plate-reader with injectors | Tomáš Mráček | |
Reader Victor X4 | 2011 | 14 | D 121 | Luminescence, Fluorescence Intensity (both top and bottom reading), UV-absorbance (UV-VIS) and Time-Resolved Fluorescence technologies | Jan Jakubík | |
Reader Synergy HT | 2008 | 75 | G C.1.09 | single-channel absorbance, fluorescence (400/30, 460/40, 485/20, 508/20, 516/20, 528/20, 560/15, 590/20) and luminiscence microplate reader. |
Equipment | Year | Dept | Location | Use for | Manager | |
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ISS PC1 spectrofluorimeter |
2006 | 13 | D 125 | photon counting steady-state fluorescence | Marek Vrbacký |
Equipment |
Year |
Dept. | Location | Use for |
Manager |
|
Beckman Optima XL-100K | 2001 | 33 | D 024 | floor model, max speed 100,000 rpm, rotors: 35 (retired), 45 Ti, 50.3 Ti, 70 Ti, 70.1 Ti, 90 Ti, NVT 90, SW 28, SW 41 Ti | Lenka Roubalová | |
Beckman Optima L-90 | 1996 | 13 | DaII 114 | floor model, max speed 90,000 rpm, rotors: same as for Optima XL-100K | Marek Vrbacký | |
Beckman Optima MAX 130K | 2007 | 33 | D 224 | benchtop model, max speed 130,000 rpm (1,019,000g with MLA-130 rotor), rotors: MLA-80, MLA-130, TLA-100, TLA-110, MLN-80 | Lenka Roubalová | |
Further info on Ultracentrifuges, Rotors, and Tubes | ||||||
Biomaterials | ||||||
Watson Marlow 530U IP31 drive | DAII ground floor | peristaltic pump | Elena Fillová | |||
Radiometry | ||||||
Shimadzu UV-1700 | 2004 | 96 | E 008 | spectrophotometer | Karla Bohuhová | |
Sample preparation | ||||||
Trans-Blot Turbo Transfer System | 2024 | 25 | D 248 | |||
Imaging systems | ||||||
Odyssey Classic IR scanner | 2004 | 13 | DaII 112 | Quantification of the signal of near-infrared fluorophores - Infrared Western blot, In-gel Western analysis, Coomassie-stained gels, SYTO 60, In-Cell Western analysis, in-vivo imaging | Kateřina Tauchmannová | |
Odyssey CLx IR scanner | 2018 | 13 | DaII 131b | Quantification of the signal of near-infrared fluorophores - Infrared Western blot, In-gel Western analysis, Coomassie-stained gels, SYTO 60, In-Cell Western analysis, On-Cell Western analysis, Protein array, ELISA, small animals imaging, tissue imaging | Kateřina Tauchmannová | |
Bio-Rad Molecular Imager Pharos FX scanner | 2009 | 22 | D 237 | imaging complex fluorescence applications | Adam Eckhardt | |
ELx808 plate reader (Biotek) | 2015 | 16 | D 324 | measurements of absorbance (filters) in 96-well plates, shaking, regulation of temperature | Olga Zimmermannová | |
EL808 plate reader (Biotek) | 2003 | 16 | D 324 | measurements of absorbance (filters) in 96-well plates, shaking, regulation of temperature | Olga Zimmermannová | |
Plate reader (Biotek) | 2003 | 16 | D 324 | measurements of absorbance (filters) in 96-well plates, shaking, regulation of temperature | Olga Zimmermannová | |
Eppendorf Mastercycler Nexus | 2024 | 16 | D 324 | PCR cycler, 0.2ml and 0.5 ml PCR tubes | Olga Zimmermannová | |
Eppendorf Mastercycler Pro | 2016 | 16 | D 324 | PCR cycler, 0.2ml PCR tubes | Olga Zimmermannová | |
Eppendorf Mastercycler Nexus Gradient | 2020 | 16 | D 324 | PCR cycler, 0.2ml or 0.5ml PCR tubes, temperature gradient | Olga Zimmermannová | |
Reader Cytation 3 (Biotek) | 2016 | 16 | D 324 | measurements of absorbance, fluorescence (filters, monochromator), luminescence, dual reagent injectors, imaging – objectives 4x and 20x, BF, DAPI, GFP, RFP, laser autofocus; adaptors – slides, Petri dish, gas controler | Olga Zimmermannová | |
Reader Synergy H1M (Biotek) | 2024 | 16 | D 324 | measurements of absorbance, fluorescence, luminescence (monochromator) | Olga Zimmermannová |