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Revolutionizing Cell Sorting

26. 6. 2024

The Institute of Biophysics of the Czech Academy of Sciences (IBP CAS) welcomed the new cutting-edge spectral flow cytometry sorter from BD Biosciences. This makes IBP CAS the first institution in the Czech Republic and the entire region to adopt this groundbreaking device. The BD Biosciences FACSDiscover™ S8 Cell Sorter with BD CellView™ Image Technology can perform precise cell sorting using real-time image analysis of cells or fluorescent signals from any of 78 detectors.

Thanks to the support of The Czech Republic Academy of Sciences, we can use and offer this technology for new scientific exploration and discovery in immunology, cell, and cancer biology.

The S8 is equipped with five lasers (349, 405, 488, 561, and 637 nm), 78 algorithmically optimized fluorescent APDs that cover the full spectrum, and a Self-Aware System for automatic spectral unmixing. The emission spectrum of each fluorochrome is captured across the various detectors and then combined to create a spectral signature that integrates emission data from all five excitation wavelengths.

BD CellView™ Image Technology allows for the composition of images from laser beamlets, enabling real-time imaging for sorting purposes. This technology permits sorting based on these imaging parameters while integrating and upgrading traditional fluorescent parameters. The image module employs a blue laser and generates three scatter parameters along with three fluorescent parameters. A short video about BD CellView™ Image Technology.

 Key features:

  • Visual, real-time inspection during analysis and sorting
  • High dimensional spectral analysis
  • Flexible 6-way sorting
  • Index flow and cell imaging data
  • Combination of image features and flow parameters


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