Kinetic fluorescence imaging technique was used to predict post-harvest damage in lemons caused by the infection of fungi Penicillium digitatum. We were able to distinguish between the mold-infected areas and mechanically damaged areas (Nedbal et al. 2000b).

In Soukupová et al. (2003) a new detection limit for plant exposure to low levels of destruxins (fungal phytotoxins of Alternaria brassicae) was set. Here, we for the first time presented a simple general experimental algorithm that can be used to identify the combination of fluorescence parameters providing high contrast between the affected and unaffected plants or plant segments. This simple algorithm was further used to prove that the activity of roseotoxin, fungal secondary metabolite of Trichothecium roseum, is similar to that of destruxins (Žabka et al. 2005, in press).

Similar approach was used in pre-symptomatic visualization of beans infected by two pathovars of Pseudomonas syringae, Nicotiana benthamiana plants infected by tobamoviruses PMMoV-I and PMMoV-S (Pepper mild mottle virus – Italian and Spanish strain) and Nicotiana tabbacum plants infected by TMV virus - cooperation with Department Biochemistry and Cell and Molecular Biology of Plants, Experimental Station of Zaidín Spanish Academy of Sciences.

In parallel, in cooperation with the Agricultural Faculty of the University of South Bohemia and the Institute of Chemical Technology we studied the effect of titanium on photosynthesis.