^^^
Dr. Ladislav Nedbal
Degree
Associate Professor (Doc.) in physics, Charles University, Prague 2004
Doctor of Natural Sciences (RNDr) in physics, Charles University, Prague 1981
Position
head of the Workgroup of Mathematical Biology
ÚSBE and of the Institute of Physical Biology
CEO of Photon Systems Instruments, Ltd.
Zámek 136, CZ-37333 Nové Hrady
Phone: +420 386361231
Fax: +420 386361231
nedbal@nh.usbe.cas.cz
http://www.e-photosynthesis.org
Current projects
European Community FP6-2005-Life Sciences, Genomics and Biotechnology for Health (contract negotiation for 2007-2011): AUTOSCREEN for cell based high-throughput and high-content gene function analysis and drug discovery screens
GAČR 206/05/0894 (2005-2007): Technical and methodical platform for monitoring and manipulation of biological regulation in plants, algae and cyanobacteria.
Czech Ministry of Education, Sports and Youth, Research Program MSM6007665808
European Space Agency: FLuorescence EXplorer (FLEX)
Functional Dynamics in Complex Chemical and Biological Systems (FUNCDYN) with VSCHT Praha
I am interested in interaction between a complex biological system and its dynamic environment. The interaction is typically far from equilibrium with strong non-linear features. We apply multidimensional (e.g., 3-D space, time, spectrum) experimental methods to capture the resulting dynamics. Systemic approach is used to construct models exhibiting dynamic features homologous to the experiment (e-photosynthesis). The dominant model process of my research is plant photosynthesis with chlorophyll fluorescence emission used as a reporter signal.
Past Projects
The earlier results that I value most are:
1. Identification of the role of Cyt b559 in photoprotection of plants (Nedbal, L., Samson, G. and Whitmarsh, J. (1992) Redox state of a one-electron component controls the rate of photoinhibition of Photosystem II. Proc.Natl.Acad.Sci.
2. My lab was the first to propose that mycosporine-like amino acids protect algae against UV-B radiation (Xiong F., Lederer F., Lukavský J. and Nedbal L. (1996) Screening of freshwater algae (Chlorophyta, Chromophyta) for ultraviolet-B sensitivity of the photosynthetic apparatus. J.Plant Physiol. 148: 42-48 / Xiong FS, Komenda J, Kopecký J, et al. (1997) Strategies of ultraviolet-B protection in microscopic algae. Physiol plantarum 100: (2) 378-388).
3. With Martin Trtílek, I am co-founder of Photon Systems Instruments, spol. s r.o.. We proposed and constructed numerous novel instruments that are operated by many distinguished users worldwide.
4. We identified new chlorophyll fluorescence transient in harmonically modulated irradiance (Nedbal, L., Brezina, V., Adamec, F., Štys, D., Oja, V., Laisk, A. and Govindjee: Negative feedback regulation is responsible for the non-linear modulation of photosynthetic activity in plants and cyanobacteria exposed to a dynamic light environment (2003) Biochim.Biophys.Acta: Bioenergetics 1607: 5-17 / Nedbal,L., B?ezina,V, ?ervený, J. and Trtílek, M. (2005) Photosynthesis in dynamic light: Systems biology of unconventional chlorophyll fluorescence transients in Synechocystis sp. PCC6803. Photosynth.Res. 84: 99-106).
5. My laboratory was the first research pioneer in Nové Hrady starting in 2000 an endeavor leading to the present Academic and
6. In 2002, we designed and organized start-up of Schola ludus program of graduate education in Nové Hrady.
7. In 2002, we organized the first international workshop in Nové Hrady as the foundations to the present
8. In 2006, we hosted Systems Biology Mark-Up Language (SBML) Hackathon http://sbml.org/workshops/, 7-9 April and Workshop on Emergent Dynamic Properties of Photosynthetic Organisms, 30 July - 5 August
Links
Visit our spin-off Photon Systems Instruments www.psi.cz
Recent publicity in Czech TV Planeta veda
Publications
- Nedbal L., Trtílek M., Červený J., Komárek O., Pakrasi H. (2008) A photobioreactor system for precision cultivation of photoautotrophic microorganisms and for high-content analysis of suspension dynamics. In: Biotechnology and Bioengineering. Vol 100 (5), pp. 902-910.
- Pineda, M., Soukupová, J., Matouš, K., Barón, M. and Nedbal, L. (2008) Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants. In: Photosynthetica. Vol 46 (3), pp. 441-451.
- Nedbal L, Cerveny J, Rascher U, et al. (2007) E-photosynthesis: a comprehensive modeling approach to understand chlorophyll fluorescence transients and other complex dynamic features of photosynthesis in fluctuating light. In: Photosynthesis Research. Vol 93 (1-3), pp. 223-234.
- Berger S, Benediktyová Z, Matous K, Bonfig K, Mueller MJ, Nedbal L, Roitsch T. (2007) Visualization of dynamics of plant-pathogen interaction by novel combination of chlorophyll fluorescence imaging and statistical analysis: differential effects of virulent and avirulent strains of P. syringae and of oxylipins on A. thaliana. In: J Exp Bot. Vol 58 (4), pp. 797-806.
- Matous K, Benediktyová Z, Berger S, Roitsch T, Nedbal L. (2006) Case study of combinatorial imaging: what protocol and what chlorophyll fluorescence image to use when visualizing infection of Arabidopsis thaliana by Pseudomonas syringae?. In: Photosynth Res. Vol 90 (3), pp. 243-253.
- Žabka, M., Drastichová, K., Jegorov, A., Soukupová, J. and Nedbal, L. (2006) Direct evidence of plant-pathogenic activity of fungal metabolites of Trichothecium roseum on apple. In: Mycopathologia. Vol 162 (1), pp. 65-68.
- Rascher U. and Nedbal, L. (2006) Dynamics of plant photosynthesis in fluctuating light. In: Current Opinions in Plant Biology. Vol 9, pp. 671-678.
- Nedbal, L. and Koblížek, M. (2006) Chlorophyll fluorescence as a reporter on in vivo electron transport and regulation in plants.. In: “Biochemistry and Biophysics of Chlorophylls”., pp. 507-519. (Eds: B. Grimm, R. Porra, W. Rüdiger, H. Scheer), Springer, ISBN 1-4020-4515-8.
- Nedbal,L., Březina,V., Červený,J., Trtílek,M. (2005) Photosynthesis in dynamic light: systems biology of unconventional chlorophyll fluorescence transients in Synechocystis sp. PCC 6803. In: Photosynthesis Research. Vol 84, pp. 99-106.
- Nedbal L, Březina V, Adamec F, Štys D, Oja V, Laisk A, Govinjee (2003) Negative feedback regulation is responsible for the non-linear modulation of photosynthetic activity in plants and cyanobacteria exposed to a dynamic light environment. In: BIOCHIM BIOPHYS ACTA. Vol 1607, pp. 5-17.
- Soukupová J, Smatanová S, Nedbal L, Jegorov A (2003) Plant response to destruxins visualized by imaging of chlorophyll fluorescence. In: PHYSIOL PLANTARUM. Vol 118, pp. 1-8.
- Nedbal L, Brezina V (2002) Complex metabolic oscillations in plants forced by harmonic irradiance. In: BIOPHYS J. Vol 83 (4), pp. 2180-2189.
- Koblížek, M., Kaftan, D., Whitmarsh J. and Nedbal, L. (2001) On the relationship between the non-photochemical quenching of the chlorophyll fluorescence and the Photosystem II light harvesting efficiency. A repetitive flash fluorescence induction study. In: Photosynth. Res.. Vol 68 (2), pp. 141-152.
- Kupper H, Setlik I, Trtilek M, Nedbal L (2000) A microscope for two-dimensional measurements of in vivo chlorophyll fluorescence kinetics using pulsed measuring radiation, continuous actinic radiation, and saturating flashes. In: Photosynthetica. Vol 38 (4), pp. 553-570.
- Šprtová, M., Marek, M.V. and Nedbal, L. (2000) Effect of enhanced UV-B radiation on chlorophyll a fluorescence parameters in Norway spruce needles. In: Journal of Plant Physiology. Vol 156, pp. 234-241.
- Wang QJ, Nedbal L, Whitmarsh J (2000) Investigating the puzzle of oxygen evolution in cyanobacteria lacking photosystem I. In: BIOPHYS J. Vol 78 (1), pp. 81-81.
- Nedbal L., Soukupová J., Kaftan D., Whitmarsh J. and Trtílek M. (2000) Kinetic imaging of chlorophyll fluorescence using modulated light. In: Photosynthetica. Vol 66, pp. 1-2.
- Nedbal, L., Soukupova, J., Kaftan, D., Whitmarsh, J. and Trtilek, M. (2000) Modulated-light fluorescence imager. In: Photosynthetica. Vol 66, pp. 3-12.
- Nedbal L, Soukupova J, Whitmarsh J, Trtilek M (2000) Postharvest imaging of chlorophyll fluorescence from lemons can be used to predict fruit quality. In: Photosynthetica. Vol 38 (4), pp. 571-579.
