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An illustration of the principle of the Solvent Relaxation method.

Selected publications reporting principles and applications of the solvent relaxation technique:

  1. Sykora, J., P. Slavicek, P. Jungwirth, J. Barucha, and M. Hof, Time-dependent stokes shifts of fluorescent dyes in the hydrophobic backbone region of a phospholipid bilayer: Combination of fluorescence spectroscopy and ab initio calculations. Journal of Physical Chemistry B, 2007. 111(21): p. 5869-5877,

  2. Jurkiewicz, P., A. Olzynska, M. Langner, and M. Hof, Headgroup hydration and mobility of DOTAP/DOPC bilayers: A fluorescence solvent relaxation study. Langmuir, 2006. 22(21): p. 8741-8749,

  3. Sykora, J., P. Jurkiewicz, R.M. Epand, R. Kraayenhof, M. Langner, and M. Hof, Influence of the curvature on the water structure in the headgroup region of phospholipid bilayer studied by the solvent relaxation technique. Chemistry and Physics of Lipids, 2005. 135(2): p. 213-221,

  4. Jurkiewicz, P., J. Sykora, A. Olzynska, J. Humplickova, and M. Hof, Solvent relaxation in phospholipid bilayers: Principles and recent applications. Journal of Fluorescence, 2005. 15(6): p. 883-894,

  5. Humpolickova, J., M. Stepanek, K. Prochazka, and M. Hof, Solvent relaxation study of pH-dependent hydration of poly(oxyethylene) shells in polystyrene-block-poly(2-vinylpyridine)-block-poly(oxyethylene) micelles in aqueous solutions. Journal of Physical Chemistry A, 2005. 109(48): p. 10803-10812,

  6. Sykora, J., V. Mudogo, R. Hutterer, M. Nepras, J. Vanerka, P. Kapusta, V. Fidler, and M. Hof, ABA-C-15: A new dye for probing solvent relaxation in phospholipid bilayers. Langmuir, 2002. 18(24): p. 9276-9282,

  7. Sykora, J., P. Kapusta, V. Fidler, and M. Hof, On what time scale does solvent relaxation in phospholipid bilayers happen? Langmuir, 2002. 18(3): p. 571-574,

  8. Hutterer, R. and M. Hof, Probing ethanol-induced phospholipid phase transitions by the polarity sensitive fluorescence probes Prodan and Patman. Zeitschrift Fur Physikalische Chemie-International Journal of Research in Physical Chemistry & Chemical Physics, 2002. 216: p. 333-346,

  9. Hutterer, R. and M. Hof, Dynamics in diether lipid bilayers and interdigitated bilayer structures studied by time-resolved emission spectra, decay time and anisotropy profiles. Journal of Fluorescence, 2001. 11(3): p. 227-236,

  10. Egelhaaf, H.J., B. Lehr, M. Hof, A. Hafner, H. Fritz, F.W. Schneider, E. Bayer, and D. Oelkrug, Solvation and solvent relaxation in swellable copolymers as studied by time-resolved fluorescence spectroscopy. Journal of Fluorescence, 2000. 10(4): p. 383-392,

  11. Hutterer, R., F.W. Schneider, W.T. Hermens, R. Wagenvoord, and M. Hof, Binding of prothrombin and its fragment 1 to phospholipid membranes studied by the solvent relaxation technique. Biochimica Et Biophysica Acta-Biomembranes, 1998. 1414(1-2): p. 155-164,

  12. Hutterer, R., A.B.J. Parusel, and M. Hof, Solvent relaxation of Prodan and Patman: A useful tool for the determination of polarity and rigidity changes in membranes. Journal of Fluorescence, 1998. 8(4): p. 389-393,

  13. Hof, M. and R. Hutterer, Solvent relaxation of fluorescent labels as a new tool for the detection of polarity and rigidity changes in membranes. Czechoslovak Journal of Physics, 1998. 48(4): p. 435-441,

  14. Hutterer, R., F.W. Schneider, H. Lanig, and M. Hof, Solvent relaxation behaviour of n-anthroyloxy fatty acids in PC-vesicles and paraffin oil: A time-resolved emission spectra study. Biochimica Et Biophysica Acta-Biomembranes, 1997. 1323(2): p. 195-207,

  15. Hutterer, R., F.W. Schneider, and M. Hof, Anisotropy and lifetime profiles for n-anthroyloxy fatty acids: A fluorescence method for the detection of bilayer interdigitation. Chemistry and Physics of Lipids, 1997. 86(1): p. 51-64,

  16. Hof, M., P. Lianos, and A. Laschewsky, An amphiphilic hemicyanine dye employed as a sensitive probe of water in reverse AOT micelles. Langmuir, 1997. 13(8): p. 2181-2183,

  17. Hof, M. and P. Lianos, Structural studies of thin AOT films by using the polarity fluorescent probe coumarin-153. Langmuir, 1997. 13(2): p. 290-294,

  18. Hutterer, R., F.W. Schneider, H. Sprinz, and M. Hof, Binding and relaxation behaviour of Prodan and Patman in phospholipid vesicles: A fluorescence and H-1 NMR study. Biophysical Chemistry, 1996. 61(2-3): p. 151-160,

  19. Hof, M., R. Hutterer, N. Perez, H. Ruf, and F.W. Schneider, Influence of Vesicle Curvature on Fluorescence Relaxation Kinetics of Fluorophores. Biophysical Chemistry, 1994. 52(2): p. 165-172,


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