Laboratory of Biology of Cytoskeleton

IMG ASCR, v.v.i


Gallery of immunofluorescence stainings


3T3-interphase.jpg (76542 bytes) 3T3-mitosis.jpg (27280 bytes)

Mouse embryonal fibroblast 3T3. Interphase cell.

Mouse embryonal fibroblast 3T3. Metaphase, anaphase and telophase.
Tubulin stained with polyclonal antibody (green), gamma-tubulin stained with antibody TU-30 (red), DNA (blue). Tubulin stained with polyclonal antibody (green), gamma-tubulin stained with antibody TU-30 (red), DNA (blue).
 

 

3T3-microinjection.jpg (27160 bytes) 3T3-vinblastine.jpg (21422 bytes)

Mouse embryonal fibroblast 3T3.

Mouse embryonal fibroblast 3T3 treated with anti-mitotic drug vinblastine.

Cells were microinjected with antibody TU-07 recognizing epitope within C-terminal structural domain of alpha-tubulin (green). Preparation was thereafter fixed and stained with polyclonal antibody against tubulin (red)  and DNA-binding dye (blue). 

Tubulin paracrystals stained with antibody TU-01 against alpha-tubulin (red), vimentin coils stained with antibody VI-01 against vimentin (green), DNA (blue). 

 

 

RBL.jpg (30309 bytes) erythrocytes-vimentin.jpg (89509 bytes)
Rat basophilic leukemia cells RBL. Chicken postnatal erythrocytes.

Alpha-tubulin stained with antibody TU-01 against alpha-tubulin (green), vimentin stained with antibody VI-01 (red), DNA (blue).

Tubulin stained by polyclonal antibody (red), vimentin stained with antibody VI-10 (green),  DNA (blue).
 

 

neurons-tubulin.jpg (30908 bytes) Leydig cell-actin.jpg (30609 bytes)
Primary culture of  rat neurons and glial cells. Mouse Leydig cell in primary culture.
Tubulin stained by polyclonal antibody (green), neuron-specific class-III beta tubulin stained with antibody TU-20 (red), DNA (blue). Actin fibers stained with  labelled-phalloidin (red), DNA (blue).