Hybridoma technology
Preparation of monoclonal antibodies against selected proteins (sperm, seminal plasma, trophoblast, different pollutants).

Immunochemical and immunocytochemical methods
ELISA, immunodetection on the blot, indirect immunofluorescence microscopy (fluorescent and confocal microscopy), immunolabeling (electron microscopy).

Chromatographic methods
Affinity and size exclusion chromatographies, high-performance liquid chromatography with reversed phase.

Electrophoretic methods
SDS-PAGE, 2D electrophoresis, zymographies, IEF, Western blotting.

Methods of molecular biology
Isolation of mRNA, PCR, preparation of recombinant proteins.

Binding studies
ELBA (Enzyme-Linked Binding Assay).

Sperm/zona pellucida binding test
Test for detection of binding activity of sperm proteins in vitro.