Contact

Laboratoř genové exprese

Biotechnologický ústav AV ČR

Vídeňská 1083

142 20 Prague 4

Czech Republic

Tel.:+420 241 063 623

Fax: +420 241 063 610

Institute of Biotechnology

Instruments

BioMark™ System

Fluidigm The BioMark™ The BioMark™ line of life science products are meeting the challenges of today’s high throughput research environment through integrated fluidic circuits known as dynamic arrays and digital arrays. These innovative products allow scientists to practice tried-and-true techniques, such as TaqMan® assays, while realizing a previously unachievable throughput. The advantages of the BioMark system include:
  • Savings. Researchers save on reagents, pipette tips, time, and on the upkeep of expensive liquid-handling robots.
  • More Data. Far more data are generated compared to microwell chips, and with no pre-spotting required.

QIAcube

Qiagen QIAcube The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. No change of purification chemistry is required, assuring fast startup and immediate results. All steps in the purification procedure are fully automated – and up to 12 samples can be processed per run.

For fully automated sample prep using spin-column kits

  • Automation of trusted QIAGEN spin-column kits
  • Elimination of manual processing steps
  • Purification of DNA, RNA, or proteins
  • More free time with affordable automated processing
  • Standardized results and increased productivity

Qiagen Video

2100 Bioanalyzer

Agilent Agilent The Agilent 2100 Bioanalyzer is the most successful microfluidics-based platform available commercially, offering solutions for the analysis of DNA, RNA, proteins and cells. It answers your questions within 30 minutes, delivering automated, high quality digital data.
  • Faster than gels (digital data in 30 minutes for quality and quantity of up to 12 samples)
  • Reproducible and complete digital data
  • Quantification of each individual fragment per sample offers purity results
  • Compliance Services allow work within regulated environments (e.g. QA/QC labs)

Experion Automated Electrophoresis Station

Bio-Rad Experion Automated Electrophoresis Station The Experion automated electrophoresis station performs all of the steps of gel-based electrophoresis in one compact, durable unit.

It automates analysis by combining electrophoresis, staining, destaining, band detection, and imaging into a single, 30 minute step. It's an electrophoresis cell, power supply, and imager built into a single device.

Designed for Easy, Precise Operation

  • Highly accurate laser provides precise fluorescent detection
  • USB port allows easy installation and maximum usability
  • Readily accessed and removable electrode manifold allows easy maintenance
  • Built-in power supply reduces cost and saves benchspace

Experion Training Video: Chip Loading.

Bio-Rad CFX96 Real Time PCR Instrument

Bio-Rad Bio-Rad CFX96 Real Time PCR Instrument Bio-Rad’s next-generation PCR instruments meet all your PCR needs — whether you are designing your first experiment or running a high-throughput laboratory and analyzing large gene expression studies. The CFX96 real-time PCR detection system builds on the power and flexibility of the C1000™ thermal cycler, adding an easy-to-install interchangeable reaction module to create an exceptional real-time PCR system. The system’s unsurpassed thermal performance plus its innovative optical design produce accurate, reliable data, and the powerful yet intuitive software accelerates every step of your real-time PCR research, shortening the time between getting started and getting great results.

Eppendorf centrifuga 5804

Eppendorf Eppendorf centrifuga 5804 Eppendorf Model 5804R Multipurpose Centrifuges feature powerful, maintenance-free motors, and they accommodate a variety of rotors for unparalleled application versatility. They are three centrifuges in one: a high capacity general purpose centrifuge for cell harvesting; a high-speed centrifuge for separating cell lysates; and a micro centrifuge for DNA precipitations.Product features Interchangeable swing-bucket and high-speed fixed-angle rotors spin microcentrifuge tubes, popular glass and plastic tubes, microplates, and blood collection tubes Variable speed, maintenance-free motor spins up to 14,000 rpm, 20,800 x g Low profile 11-inch access height for easy sample loading Automatic rotor recognition senses rotor type to set maximum allowable speed-ensures safety.

Eppendorf centrifuga 5415R

Eppendorf Eppendorf centrifuga 5415R

Centrifuge 5415 R enables rapid and reliable refrigerated centrifugation in a temperature range between 0 °C and 40 °C. Temperature sensitive samples are maintained safely at 4 °C, even at maximumn speed. With the aid of the Fast Cool function, an almost chilly 4 °C can be attained within a mere 16 minutes. It has the indestructible maintenance-free drive, which requiresonly 13 seconds to bring the rotor to the maximum speed of 13,200 rpm (max. rcf: 16,110 x g).

Eppendorf R epMotion T 5070 Workstation

Eppendorf Eppendorf R epMotion T 5070 Workstation The Eppendorf epMotion 5070 workstation enables fast and reproducible liquid handling by way of pipetting, dispensing, and other dispensing variants. With its great similarity to manual pipettes and to the familiar manual work method, pipetting sequences and protocols previously carried out manually can now be transferred quickly and easily to the epMotion 5070 workstation. Tried and true pipetting procedures have been maintained and standard techniques are quickly and easily transferred. Extensive experience with other liquid handling platforms and long training times are not needed.Valuable bench space is saved by using the integrated control panel instead of a PC. Operate the workstation using the keyboard of the connected control panel. The connected mouse enables free movement of the cursor over the integrated screen. Thus, an almost-intuitive operation with greater flexibility in the application is made possible. The user is led step by step through the software and prompted to carry out the necessary actions.

The following steps take place fully automatically following the insertion of the dispensing tool: the dispensing tool attaches the pipette tip; the liquid to be isolated is taken up from a supply and dispensed into one or more target tubes. Normally, the dispensing is concluded following the ejection of the pipette tip. Optical sensors control and ensure the reliability of the procedure:

  • Testing of liquid quantities
  • Testing of the existing pipette tip type with the programming
  • Testing of the existing tubes with the programming

The epMotion 5070 workstation enables quick filling of plates in the 384-well scale. It is also possible to fill an MTP 384-well from other formats (eg, 4 x MTP 96-well).

Listen to the Eppendorf song.

Mastercycler ep realplex real-time PCR system

Eppendorf Mastercycler ep realplex real-time PCR system The Eppendorf Mastercycler ep realplex system1 is a licensed real-time thermal cycler for quantitative, real-time PCR applications. The realplex combines the advanced thermal cycling technology of Eppendorf’s Mastercycler2 ep gradient thermocyclers with intuitive, multipurpose software and an expertise in optical systems. A modular design allows you to choose from two 96-well options—a fast aluminum gradient block or the ultrahigh-speed gradient S. Two optical modules—realplex2 or realplex4—permit the use of nearly all fluorescent dyes used in real-time PCR.

Eppendorf Mastercycler realplex video.

NanoDrop 1000

Thermo NanoDrop 1000 The Thermo Scientific NanoDropTM 1000 Spectrophotometer enables highly accurate UV/Vis analyses of 1 ul samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions. You’ll find yourself making fewer dilutions and taking more readings than ever before, essentially becoming a more quantitative laboratory.

The NanoDrop 1000 offers many benefits:

  • Small samples: designed for 1 ul samples
  • Large dynamic range: measures 2-3700 ng/ul (dsDNA) on a single sample. No need to change out cuvettes or caps.
  • High absorbance capability: 50 times that of traditional spectrophotometers
  • No Dilutions for most samples
  • No extra costs for consumables
  • Full Spectrum (220-750nm)
  • 10 second measurement time
  • No cuvettes or capillaries
  • Sample is recoverable
  • Small size (size of a tissue box)
  • Easy set up (plug and play)
  • Software designed for life sciences
  • Free software upgrades

You can watch ilustrative movie.

Rotor Gene 6000

Ilustration Ilustration

The Rotor-Gene 6000 is unlike any other real-time system. It uses a unique centrifugal rotary design, ideal for even the most demanding requirements of real-time thermo-optical analyses. What sets the Rotor-Gene apart is its amazing precision; the result of near-perfect well-to-well thermal and optical uniformity. Its design also enables a fast data acquisition rate, a capability now proving to be essential for advanced applications such as HRM (high-resolution melt).

  • Unique centrifugal rotary format
  • Uniformity: ± 0.01°C
  • Resolution: 0.02°C (smallest programmable and reported thermal increment)
  • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels
  • High resolution melt (HRM) application
  • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc
  • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC, and free software upgrades from Corbett Download Centre.

Watch a 3D animation of the Rotor-Gene 6000 via the You Tube.

StepOne™ Real-Time PCR System

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The StepOne™ Real-Time PCR System delivers remarkable plug-and-play convenience and uncompromising performance. It supports the full range of TaqMan® assays while providing ease-of-use and a small footprint. Each system offers intuitive software, fast runs (< 40 minutes) and standard runs (< hours), and are configured for PC-free or networked operation. This factory-calibrated system makes it easy to step up to high performance real-time PCR.

  • Surprisingly affordable
  • 48-wells for lower throughput
  • 3 colors for basic applications research
  • Easily upgradeable to the StepOnePlus™ System

7500 Fast Real-Time PCR System

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The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.

  • Specially designed Fast block ensures thermal uniformity at top speeds
  • Faster ramp rates enable rapid results without compromising extension times or assay quality
  • Fast optical plates ensure excellent precision in 10-30 uL reaction volumes
  • Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate
  • Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System
  • The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes
  • High Resolution Melting Analysis available

Quick Gene-Mini 80

Fujifilm Fujifilm

The key technology distinguishing the QuickGene systems from comparable products is Fujifilm’s patented 80 µm porous membrane, which selectively binds nucleic acids. This ultra-thin membrane with uniformly fine pores and a large specific binding surface ensures high yield and outstanding purity. The membrane’s unique features allow DNA extraction using air pressure, with no need for centrifugation.

  • Genomic DNA can be extracted manually from 8 samples simultaneously in just 12 minutes
  • The QuickGene systems can now be applied as CE IVD marked pre-analytic method for molecular diagnostics