Contact

Laboratoř genové exprese

Biotechnologický ústav AV ČR

Vídeňská 1083

142 20 Prague 4

Czech Republic

Tel.:+420 241 063 623

Fax: +420 241 063 610

Institute of Biotechnology

Gene Expression Services

Core Facility

The Quantitative PCR Core Facility was founded in 2009 at the Institute of Biotechnology AS CR, v.v.i., it is a part of Laboratory of Gene Expression, which is under supervision of Prof. Mikael Kubista. The Quantitative PCR Core Facility provides high-throughput gene and transcript analysis using real-time quantitative PCR and especially a unique high-throughput instrument Biomark from Fludigm. The PCR core facility has also the following major equipment available: 5 thermocyclers: Realplex Mastercycler from Eppendorf, CFX96 from Bio-Rad, StepOne Real-Time PCR System and 7500 Fast Real-Time PCR System from Applied Biosystems and a rotor based Rotor Gene 6000 from Qiagen. 7500 Fast Real-Time PCR System and Rotor Gene 6000 can be used for High resolution melt analysis. Samples can be processed and assay plates assembled utilizing a robotic liquid handling workstation Eppendorf R epMotion T 5070 Workstation. The robot is capable of loading 96-well and 384-well plates in a timely manner with great precision. For a quality and quantity controls of samples, NanoDrop ND-1000 Spectrophotometer, Experion Automated Electrophoresis Station from Bio-Rad and 2100 Bioanalyzer from Agilent Technologies are available

The real-time PCR Core Facility, headed by Vlasta Korenkova, Ph.D., offers a full complement of real-time PCR services not only for internal users but also for researches from other academic institutions. Our services include:

  • One-on-one consulting on experimental design
  • Automated RNA/DNA isolation using QIAcube(Qiagen) or Quick Gene-Mini 80 (Fujifilm)
  • Probe/primer design
  • Quantification of your RNA/DNA samples: Nanodrop quantification
  • Determining quality of RNA/DNA samples: Experion Bioanalyzer (Bio-Rad) or 2100 Bioanalyzer (Agilent Technologies)
  • Reverse transcription
  • Preamplification
  • Setting up your real-time PCR experiment (96 reaction plate). Instruments available: Realplex Mastercycler (Eppendorf), CFX96 (Bio-Rad), StepOne Real-Time PCR System and 7500 Fast Real-Time PCR System (Applied Biosystems), Rotor Gene 6000 (Qiagen)
  • Setting up Biomark real-time PCR experiment. Six different arrays are available, see Biomark page.
  • High resolution melt (7500 Fast Real-Time PCR System and Rotor Gene 6000)
  • Data analysis
  • Training to use the method and equipment: TATAA qPCR courses