Biomark

The core facility offers high-throughput gene and transcript quantification services with real-time quantitative PCR based on the Biomark microfluidic platform from Fluidigm, USA, with which 48 (assays) × 48 (samples) = 2,304 or 96 x 96 = 9,216 reactions can be processed in a single run. Available is also digital array technology for absolute quantification of PCR targets.

• Digital Array 12.765
• Digital Array 48.771

Prices for use of Biomark

For pricing, please see pdf document.

Biomark references

For references on Biomark, please see pdf document.

Samples

If it is possible, bring cDNA samples, do RT with random hexamers mixed with oligo dT. However, the input samples have to meet requirements on quality and quantity to obtain succesful result of experiment. Researches are thus required to check the quality and quantity of their RNA. The quality of the total RNA samples could be assessed by agarose gel electrophoresis or Agilent 2100 Bioanalyzer or Experion Automated Electrophoresis. On the agarose gel, there should be two bands visible without obvious smears and higer molecular weight bands. Good quality RNA will have an OD 260/280 ratio of 1.8 to 2.1 and an OD 260/230 of 1.8 or greater.

You can bring RNA samples if your target gene expression is high. Any extracted RNA must be devoid of contaminants such as salt, protein, solvents and genomic DNA. Poor quality RNA will lead to problems when performing the reverse transcription. We can process your RNA and make all necessary controls: quantity measurements (Nanodrop Spectrophotometer) and quality control (Experion Automated Electrophoresis, Agilent Bioanalyser). We will reverse transcribe your RNA into cDNA and we can perform subsequent preamplification, if it is needed. The minimal RNA concentration intended for preamplification should be at least 10 ng/ul.