Contact

Laboratoř genové exprese

Biotechnologický ústav AV ČR

Vídeňská 1083

142 20 Prague 4

Czech Republic

Tel.:+420 241 063 623

Fax: +420 241 063 610

DIČ: CZ86652036

IČ: 86652036

VAT No.: CZ86652036

Institute of Biotechnology

Gene Expression Services

Core Facility

The Quantitative PCR Core Facility was founded in 2009 at the Institute of Biotechnology AS CR, v.v.i., it is a part of Laboratory of Gene Expression, which is under supervision of Prof. Mikael Kubista. The Quantitative PCR Core Facility provides high-throughput gene and transcript analysis using real-time quantitative PCR and especially a unique high-throughput instrument Biomark from Fludigm. The PCR core facility has also the following major equipment available: 5 thermocyclers: Realplex Mastercycler from Eppendorf, CFX96 from Bio-Rad, StepOne Real-Time PCR System and 7500 Fast Real-Time PCR System from Applied Biosystems and a rotor based Rotor Gene 6000 from Qiagen. 7500 Fast Real-Time PCR System and Rotor Gene 6000 can be used for High resolution melt analysis. Samples can be processed and assay plates assembled utilizing a robotic liquid handling workstation Eppendorf R epMotion T 5070 Workstation. The robot is capable of loading 96-well and 384-well plates in a timely manner with great precision. For a quality and quantity controls of samples, NanoDrop ND-1000 Spectrophotometer, Experion Automated Electrophoresis Station from Bio-Rad and 2100 Bioanalyzer from Agilent Technologies are available

The real-time PCR Core Facility, headed by Vlasta Korenkova, Ph.D., offers a full complement of real-time PCR services not only for internal users but also for researches from other academic institutions. Our services include:

  • One-on-one consulting on experimental design
  • Automated RNA/DNA isolation using QIAcube(Qiagen) or Quick Gene-Mini 80 (Fujifilm)
  • Probe/primer design
  • Quantification of your RNA/DNA samples: Nanodrop quantification
  • Determining quality of RNA/DNA samples: Experion Bioanalyzer (Bio-Rad) or 2100 Bioanalyzer (Agilent Technologies)
  • Reverse transcription
  • Preamplification
  • Setting up your real-time PCR experiment (96 reaction plate). Instruments available: Realplex Mastercycler (Eppendorf), CFX96 (Bio-Rad), StepOne Real-Time PCR System and 7500 Fast Real-Time PCR System (Applied Biosystems), Rotor Gene 6000 (Qiagen)
  • Setting up Biomark real-time PCR experiment. Six different arrays are available, see Biomark page.
  • High resolution melt (7500 Fast Real-Time PCR System and Rotor Gene 6000)
  • Data analysis
  • Training to use the method and equipment: TATAA qPCR courses