Transporters in cell physiology

Recent Papers

2011

• Krauke Y., Sychrova H.: Cnh1 Na+/H+ antiporter and Ena1 Na+-ATPase play different roles in cation homeostasis and cell physiology of Candida glabrata.
  FEMS Yeast Res 11, 29-41 (2011) [Abstract].
• Martínez J.L., Sychrova H., Ramos J.: Monovalent cations regulate expression and activity of the Hak1 potassium transporter in Debaryomyces hansenii.
  Fung. Gen. Biol. 48, 177-184 (2011) [Abstract].
• Leandro, M.J., Sychrova H., Prista C., Loureiro-Dias M.C.: The osmotolerant fructophilic yeast Zygosaccharomyces rouxii employs two plasma-membrane fructose uptake systems belonging to a new family of yeast sugar transporters.
  Microbiology 157, 601-607 (2011) [Abstract].

2010

• Krauke Y., Sychrova H.: Chimeras between C. glabrata Cnh1 and S. cerevisiae Nha1 Na+/H+-antiporters are functional proteins increasing the salt tolerance of yeast cells.
  Folia Microbiol. 55, 435-441 (2010) [Abstract].
• Curto M., Valledor L., Navarrete C., Gutierez D., Sychrova H., Ramos J., Jorrin J.: 2-DE based proteomic analysis of Saccharomyces cerevisiae wild and K+ transport-affected mutant (trk1,2) strains at the growth exponential and stationary phases.
  J Proteom 73, 2316-2335 (2010) [Abstract].
• Maresova L., Sychrova H.: Genetic interactions between the Arl1 GTPase and intracellular Na+/H+ antiporters in pH homeostasis and cation detoxification.
  FEMS Yeast Res 10, 802-811 (2010) [Abstract].
• Huang X., Morse L.R., Xu Y., Zahradka J., Sychrova H., Stashenko P., Fan F., Batagglino R.A.: Mutational analysis of NHAoc/NHA2 in Saccharomyces cerevisiae. Biochim Biphys Acta 1800, 1241-1247 (2010) [Abstract].
• Petrezsélyová S., Zahrádka J., Sychrová H.: Saccharomyces cerevisiae BY4741 and W303-1A laboratory strains differ in salt tolerance.
  Fungal Biology (former Mycological Research) 114, 144-150 (2010) [Abstract].
• Marešová L., Hošková B.J., Urbánková E., Chaloupka R., Sychrová H.: New applications of pHluorin – measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis.
  Yeast 27, 317-325 (2010) [Abstract].
• Arino J., Ramos J., Sychrova H.: Alkali-metal-cation transport and homeostasis in yeasts.
  Microbiol. Mol. Biol. Rev. 75 (1), 95-120 (2010) [Abstract].
• Krauke Y., Sychrová H.: Four pathogenic Candida species differ in salt tolerance.
  Current Microbiology (2010) [Abstract | doi: 10.1007/s00284-010-9616-3].
• Navarrete C., Petrezsélyová S., Barreto L., Martínez J.L., Zahrádka J., Arino J., Sychrová., Ramos J.: Lack of main K+ uptake systems in Saccharomyces cerevisiae cells affects yeast cell physiological parameters both in potassium sufficient and limiting conditions.
  FEMS Yeast Res (2010) [Abstract | doi: 10.1111/j.1567-1364.2010.00630.x].

2009

• Maresova L, Muend S, Zhang YQ, Sychrova H, Rao R.: Membrane hyperpolarization drives cation influx and fungicidal activity of amiodarone.
  J. Biol. Chem 284 (5), 2795-2802 (2009) [Abstract].
• Kolecka A., Krauke Y., Bujdaková H., Sychrová H.: Subinhibitory concentrations of fluconazole increase the intracellular sodium content in both fluconazole-resistant and sensitive Candida albicans strains.
  Can J. Microbiol 55(5), 605-610 (2009) [Abstract].

2008

• Schwartzer S., Koláčná L., Lichtenberg-Fraté H., Sychrová H. , Ludwig J.: Functional expression of the voltage-gated neuronal mammalian potassium channel rat ether à go-go in yeast.
  FEMS Yeast Res. 8, 405-413 (2008) [Abstract].
• Krauke Y., Sychrova H.: Functional comparison of plasma-membrane Na+/H+ antiporters from two pathogenic Candida species.
  BMC Microbiology, 8:80 (2008) [Abstract | doi: 10.1186/1471-2180-8-80].
• Přibylová L., Papoušková K., Sychrová H.: The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiology
  Fun. Gen. Biol. 45, 1439-1447 (2008) [Abstract].

2007

• Papoušková K., Sychrová H.: Schizosaccharomyces pombe possesses two plasma membrane alkali metal cation/H+ antiporters differing in thein substrate specificity.
  FEMS Yeast Res 7, 188-195 (2007) [Abstract].
• Přibylová L., de Montigny J., Sychrová H.: Osmoresistant yeast Zygosaccharomyces rouxii: the two most studied wild-type strains (ATCC 2623 and ATCC 42981) differ in osmotolerance and glycerol metabolism.
  Yeast 24, 171-180 (2007) [Abstract].
• Papoušková K., Sychrová H.: The co-action of osmotic and high temperature stresses results in a growth improvement of Debaryomyces hansenii cells.
  Int J Food Microbiol 118, 1-7 (2007) [Abstract].
• Přibylová L., Straub M.-L., Sychrová H., de Montigny J.: Characterisation of Zygosaccharomyces rouxii centromeres and construction of first Z. rouxii centromeric vectors.
  Chromosome Res 15, 439–445 (2007) [Abstract].
• Kinclova-Zimmermannova O., Sychrová H.: Plasma membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans.
  Microboiology SGM 153, 2603-2612 (2007) [Abstract].
• Papoušková K., Sychrová H.: Production of Yarrowia lipolytica Nha2 Na+/H+ antiporter improves the salt tolerance of Saccharomyces cerevisiae.
  Folia Microbiol. 52 (6), 600-603 (2007) [Abstract].
• Přibylová L., Slaninova I., Farkas V., de Montigny J., Sychrova H.: Differences in osmotolerant and cell wall properties of two Zygosaccharomyces rouxii strains.
  Folia Microbiol 52 (3),241–245 (2007) [Abstract].
• Přibylová L., de Montigny J., Sychrová H.: Tools for genetic manipulation of Zygosaccharomyces rouxii
  FEMS Yeast Res 7, 1285-1294 (2007) [Abstract].
• Marešová L., Sychrová H.: Applications of a microplate reader in yeast physiology research.
  Biotechniques 43, 667-672 (2007) [Abstract].
• Hasenbrink G., Koláčná L., Ludwig J., Sychrová H., Kschischo M., Lichtenberg-Fraté H.: Ring test assessment of the mKir2.1 growth based assay in Saccharomyces cerevisiae using parametric models and model-free fits.
  Appl. Microbiol. Biotechnol. 73, 1212-1221 (2007) [Abstract].

2006

• Velkova K., Sychrova H.: The Debaryomyces hansenii NHA1 gene encodes a plasma membrane alkali-metal-cation antiporter with broad substrate specificity.
  Gene 369, 27-34 (2006) [Abstract].
• Flegelova H., Haguenauer-Tsapis R., Sychrova H.: Heterologous expression of mammalian Na/H antiporters in Saccharomyces cerevisiae.
  Biochim. Biophys. Acta 1760, 504-516 (2006) [Abstract].
• Kinclova-Zimmermannova O., Sychrova H.: Functional study of the Nha1p C-terminus: involvement in cell response to changes in extrenal osmolarity.
  Current Genetics 49, 229-236 (2006) [Abstract].
• Papouskova K., Sychrova H.: Yarrowia lipolytica possesses two plasma membrane alkali metal cation/H⁺ antiporters with different functions in cell physiology
  FEBS Lett. 580, 1971-1976 (2006) [Abstract].
• Kinclova-Zimmermannova O., Gaskova D., Sychrova H.: The Na⁺,K⁺/H⁺-antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae.
  FEMS Yeast Res. 6, 792-800 (2006) [Abstract].
• Pribylova L., Sychrova H.: Expression of the Saccharomyces cerevisiae MPR1 gene encoding N-Acetyltransferase in Zygosaccharomyces rouxii confers resistence to L-Azetidine-2-carboxylate
  Folia Microbiol. 51, 203-207 (2006) [Abstract].
• Kinclova-Zimmermannova O., Zavrel M., Sychrova H.: Importance of the seryl and threonyl residues of the fifth transmembrane domain to the substrate specificity of yeast plasma membrane Na+/H+ antiporters.
  Mol. Membr. Biol. 23, 349-361 (2006) [Abstract].
• Marešová L., Urbánková E., Gášková D., Sychrova H.: Measurements of plasma membrane potential changes in Saccharomyces cerevisiae cells reveal the importance of the Tok1 channel in membrane potential maintenance.
  FEMS Yeast Res 6, 1039–1046 (2006) [Abstract].
• Přibylová L., Papoušková K., Zavřel M., Souciet J.-L., Sychrova H.: Exploration of yeast alkali metal cation/H+ antiporters: Sequence and structure comparison.
  Folia Microbiol. 51, 413-424 (2006) [Abstract].
• Marešová L., Sychrová H.: Arabidopsis thaliana CHX17 gene complements the kha1 deletion phenotypes in Saccharomyces cerevisiae.
  Yeast 23, 1167-1171 (2006) [Abstract].

2005

• Hasenbrink G., Schwartzer S., Koláčná L., Ludwig J., Sychrová H., Lichtenberg-Fraté H.: Analysis of the mKir2.1 channel aktivity in potassium influx defective Saccharomyces cerevisiae strains determined as changes in growth characteristics.
  FEBS Lett. 579, 1723-1731 (2005) [Abstract].
• Marešová L., Sychrová H.: Physiological characterization of Saccharomyces cerevisiae kha1 deletion mutants.
  Molec. Microbiol. 55, 588-600 (2005) [Abstract].
• Kinclova-Zimmermannova O., Zavrel M., Sychrova H.: Identification of conserved prolyl residue important for transport activity and the substrate specificity range of yeast plasma membrane Na+/H+ Antiporters.
  J. Biol. Chem. 280, 30638 – 30647 (2005) [Abstract].
• Flegelova H., Sychrova H.: Mammalian NHE2 Na/H Exchange mediates efflux of potassium upon heterologous expression in trast.
  FEBS Lett. 579, 4733-4738 (2005) [Abstract].
• Kolacna L., Zimmermannova O., Hasenbrink G., Schwartzer S., Ludwig J., Lichtenberg-Frata H., Sychrova H.: New phenotypes of functional expression of the mKir2.1 channel in potassium-deficient Saccharomyces cerevisiae strains.
  Yeast 22, 1315-1323 (2005) [Abstract].

2004

• Kinclová-Zimmermannová O., Flegelová H., Sychrová H.: Rice Na⁺/H⁺- antiporter Nhx1 complements partially the alkali-metal-cation sensitivity of yeast strains lacking three sodium transporters.
  Folia Microbiol. 49, 519-525 (2004) [Abstract].
• Sychrová H.: Yeast as a model organism to study transport and homeostasis of alkali metal cations.
  Physiol. Res. 53, S91-S98 (2004) [Abstract].

2003

• Přibylová L., Sychrová H.: Efficient transformation of the osmotolerant yeast Zygosaccharomyces rouxii by electroporation.
  J. Microbiol. Meth. 55, 481-484 (2003) [Abstract].
• Marešová L., Sychrová H.: Physiological characterization of osmotolerant yeast Pichia sorbitophila and comparison with a putative synonym Pichia farinosa.
  Folia Microbiol. 48, 211-217 (2003) [Abstract].

2002

• Kinclová O., Potier S., Sychrová H.: The difference in substrate specificity divides the yeast alkali-metal-cation/H⁺ antiporters in two subfamilies.
  Microbiology-UK 148, 1225-1232 (2002) [Abstract].

2001

• Kinclová O., Potier S., Sychrová H.: The Candida albicans Na⁺/H⁺ antiporter transports potassium and rubidium.
  FEBS Lett. 504, 11-15 (2001) [Abstract].
• Sychrová H.: Molecular cloning and sequence analysis of the Zygosaccharomyces rouxii LEU2 gene encoding a ß-isopropylmalate dehydrogenase.
  Yeast 18, 989-994 (2001) [Abstract].
• Kinclová O., Potier S., Sychrová H.: The Zygosaccharomyces rouxii strain CBS732 contains only one copy of the HOG1 and the SOD2 genes.
  J. Biotechnol. 88, 151-158 (2001). [Abstract].
• Kinclová O., Ramos J., Potier S., Sychrová H.: Functional study of the Saccharomyces cerevisiae Nha1p C-terminus.
  Mol. Microbiology 40, 656-668 (2001) [Abstract].

2000

• Sychrová H., Braun V., Potier S., Souciet J.-L.: Organization of specific genomic regions of Zygosaccharomyces rouxii and Pichia sorbitophila: comparison with Saccharomyces cerevisiae.
  Yeast 16, 1377-1385 (2000) [Abstract].
• Sychrová H., Braun V., Souciet J.-L.: Sequence and organization analyses of Zygosaccharomyces rouxii DNA fragment containing the HIS3 gene.
  Yeast 16, 581-587 (2000). [Abstract]