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Laboratoř genové exprese
Biotechnologický ústav AV ČR
Vídeňská 1083
142 20 Prague 4
Czech Republic
Tel.:+420 241 063 623
Fax: +420 241 063 610
DIČ: CZ86652036
IČ: 86652036
VAT No.: CZ86652036
Biomark
The core facility offers high-throughput gene and transcript quantification services with real-time quantitative PCR based on the Biomark microfluidic platform from Fluidigm, USA, with which 48 (assays) × 48 (samples) = 2,304 or 96 x 96 = 9,216 reactions can be processed in a single run. Available is also digital array technology for absolute quantification of PCR targets.
Six different arrays are available:
Dynamic arrays
- GE Dynamic array 48.48
- GE Dynamic array 96.96
- GT Dynamic array 48.48
- GT Dynamic array 96.96
- Digital Array 12.765
- Digital Array 48.771
For pricing, please, contact Vlasta Korenkova: vlasta.korenkova@img.cas.cz
Our Biomark references
- Radek Sindelka, Monika Sidova, David Svec, Mikael Kubista.
Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography. Methods 51:87-91(2010). - Anders Bergkvist, Vendula Rusnakova, Radek Sindelka, Jose Manuel Andrade Garda, Björn Sjögreen,Daniel Lindh, Amin Forootan, Mikael Kubista.
Gene expression profiling – Clusters of possibilities. Methods 50:323–335(2010). - Sindelka R, Jonák J, Hands R, Bustin SA, Kubista M.
Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte. Nucleic Acids Res. 2008 Feb;36(2):387-92
Biomark single cell references
For references, please see pdf document.
Forms to fill (only in Czech)
Form for 48x48 array.
Form for 96x96 array.
How to prepare samples
If it is possible, bring cDNA samples, do RT with random hexamers or hexamers mixed with oligo dT. However, the input samples have to meet requirements on quality and quantity to obtain succesful result of experiment. Researches are thus required to check the quality and quantity of their RNA. The quality of the total RNA samples could be assessed by agarose gel electrophoresis or Agilent 2100 Bioanalyzer or Experion Automated Electrophoresis. On the agarose gel, there should be two bands visible without obvious smears and higer molecular weight bands. Good quality RNA will have an OD 260/280 ratio of 1.8 to 2.1 and an OD 260/230 of 1.7 or greater.
You can bring RNA samples if your target gene expression is high. Any extracted RNA must be devoid of contaminants such as salt, protein, solvents and genomic DNA. Poor quality RNA will lead to problems when performing the reverse transcription. We can process your RNA and make all necessary controls: quantity measurements (Nanodrop Spectrophotometer) and quality control (Experion Automated Electrophoresis, Agilent Bioanalyser). We will reverse transcribe your RNA into cDNA and we can perform subsequent preamplification, if it is needed. The minimal RNA concentration intended for preamplification should be at least 20 ng per sample.
