M. Hof started to build up the group in 1999. As a starting investment the JH-Institute bought a basic FCS Microscope (Zeiss).
In 2001 the lab was extended by additional rooms and equipment (mainly, TCSPC and steady state fluorimeters (Horiba JY). The confocal microscope was extended for Fluorescence Lifetime FCS. The growth of the laboratory was heavily supported by the home institute as well as by Czech science foundations.
In 2005 The Academy of Sciences financed the built up of a Time Resolved Single Molecule Sensitive Confocal Fluorescence Microscope (PicoQuant, Olympus) and the group became part of the newly founded Department of Biophysical Chemistry.
In 2006 the group became the coordinator of the National Research Centre "Advanced Fluorescence Microscopy in Biological and Medical Sciences" that is financed by the Ministry of Education.