Fig. 3. Human osteoblast-like MG 63 cells on day 14 after seeding on porous (A) or fibrous (B) scaffolds, made of a degradable copolymer of lactide and glycolide and designed for bone tissue engineering.
A: Summarizing picture of horizontal optical sections. The depth of cell ingrowth onside the scaffolds with pore size of 400-600 mm is indicated with spectral colors (blue signal: 0 – 60 mm, green: 80 – 160 mm, yellow: 180 – 220 mm, orange: 240 – 300 mm, red: 320 – 400 mm, violet: 420 – 480 mm).
B: MG 63 cells cultured in a dynamic perfusion cell culture system. The cell membrane is visualized with Texas Red C2-maleimide (red fluorescence), the cell nuclei with Hoechst #33342 (blue fluorescence). Confocal microscope Leica TCS SP2 AOBS, obj. 5x (A) or 10x (B).
C: Dynamic perfusion cell culture system (Provitro GmbH, Berlin
D: Detail of the perfusion chamber for the cell-material complex.