Fyziologický ústav

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19 May 09 - 5 Apr 14

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Biochemistry of Membrane Receptors

Head
Doc. RNDr. Petr Svoboda, DrSc

Scientists
RNDr. Lenka Roubalová, PhD
RNDr. Jiří Novotný, CSc
Mgr. Pavel Ostašov, PhD
Prof.RNDr. Jiří Wilhelm, PhD

Technical Assistants
Bc. Kateřina Kašparovská

Postgradual students
Mgr. Jana Brejchová
Ing. Kateřina Dlouhá
Mgr. Dmytro Kagan
Mgr. Hana Ujčíková
Ing. Miroslava Vošahlíková

Pregradual students
Bc. Kateřina Višněvská
Ladislav Metra

A: Separation of plasma membrane and mitochondrial fractions in Percoll gradient; flotation of plasma membrane fraction in sucrose gradient.
B: Determination of G protein activity - [³⁵S]GTPγS binding in Percoll-purified plasma membranes (rat brain cortex):
Comparison of the dose-response curves of agonist stimulation at a single [³⁵S]GTPγS concentration indicated the order of efficacy: baclofen (GABA_B-receptor) > DADLE (d-opioid receptor) > DAMGO (m-opioid receptor) > U-69593 (k-opioid receptor).