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MicroTime 200 made by PicoQuant, Berlin is a time resolved fluorescence microscopy worksation fine tuned to provide single molecule sensitivity. Besides advanced FLIM, FCS, FLCS and photon correlation (e.g antibunching) measurement capabilities, standard wide field fluorescence imaging is available as well.
The excitation system is based on fiber coupled pulsed laser diodes (405 nm, 440 nm, 470 nm, 532 nm, 640 nm) driven by a computer controlled driver. This makes possible to excite the sample with arbitrary multicolor pulse sequences (PIE, ALEX) when necessary.
Currently we have four parallel detection channels based on SPAD detectors. A chamber with controlled atmosphere (CO2 concentration, temperature, humidity) can be attached for in vivo measurements.
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FluoView 1000 made by Olympus is a confocal fluorescence microscopy system providing both single photon and multi-photon excitation capabilities. Besides classical confocal fluorescence imaging and Raster Image Correlation Spectroscopy (RICS), standard wide field fluorescence imaging is available as well.
The CW excitation system is based on two fiber coupled He-Ne lasers (514 and 632 nm) and a 488 nm diode laser. Multiphoton excitation is possible with Coherent Chameleon Vision II Titanium:Sapphire laser. Currently we use a filter based detection system with three descanned and two NDD channels. A chamber with controlled atmosphere (CO2 concentration, temperature, humidity) can be attached for in vivo measurements.
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Confocal fluorescence microscope for time (FLIM, FCS, FLCS) and spectrally (spectral imaging, spectral FCS) resolved fluorescence microscopy. The setup is also designed to perform super-resolution imaging by DSOM technique.
System is equipped with TCSPC card HydraHarp (PicoQuant, Berlin) and an imaging spectrograph based on Andor iXon EMCCD camera. Sample stage with a piezo actuator (PI, Karlsruhe) is used for scanning the sample.
Excitation sources available: pulsed laser diodes (405 nm, 440 nm, 470 nm, 640 nm) and continuous wave He-Ne lasers (633 nm, 543 nm)
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Our microscope setup for super-resolution imaging by PALM or STORM techniques is based on an Olympus IX71 frame, dual channel TIRF illuminator and Andor EM-CCD camera. The setup is able to perform dual color imaging using a configurable image splitter (Optosplit II with OptoMask plus from Cairn Research).
Multicolor excitation is accomplished by four CW diode lasers with 405 nm, 488 nm, 560 and 640 nm output combined into two fibers and modulated using two acousto-optic tunable filters (AOTFs).
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Time resolved fluorescence spectrometer IBH 5000U (TCSPC) used mainly for measurement of time resolved emission spectra (TRES) and time resolved fluorescence anisotropy.
Excitation sources available: pulsed laser diodes (370 nm, 440 nm, 470 nm, 640 nm), 280 nm LED diode.
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Modular steady state florescence spectrometer FluoroLog 3 (Horiba).
Light trace: Lamp Housing -> Double Monochromator -> Sample Compartment -> Double Monochromator -> Detector
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Confocal microscope ConfoCor 2 (Carl Zeiss, Jena) for Fluorescence correlation spectroscopy (FCS) equipped with time resolved fluorescence detection via TimeHarp 200 (PicoQuant, Berlin) data acquisition card enabling FLCS experiments. The instrument is on a long-term loan in the laboratory of XUV spectroscopy at Faculty of Biomedical Engineering of Czech Technical University. |
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Shimadzu UV-2600 UV-VIS-NIR absorption spectrometer with optional ISR-2600Plus integrating sphere and thermoelectrically temperature-controlled cell holder.
Equipment for sample preparation includes equipment for liposome preparation: rotary evaporator, sonicator for SUVs preparation, membrane extruders (Avestin) for LUVs preparation and chambers for electroformation of GUVs.
Furthermore, columns for gel chromatography, pH meter and analytic balance and other basic laboratory equipment are available.
A cell culture room with an incubator and a laminar box is available.
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MicroTime
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