People

Head of the laboratory

Assoc. Prof. RNDr. Eva Zažímalová, Ph.D.

zazimalova@ueb.cas.cz

Mechanisms of transport of auxins and cytokinins across the plasma membrane; auxin carrier systems and their regulation. Perception and transduction of auxin and cytokinin signals. Roles of these processes in the regulation of cell division and polar growth.

Scientists (in alphabetical order)

	Ing. Petre Ivanov Dobrev, Ph.D. dobrev@ueb.cas.cz Development of methods for the extraction, purification and determination of plant hormones using HPLC with on-line monitoring of UV-Vis, fluorescence, radioactivity and mass spectra. Metabolism of plant hormones, particularly cytokinins, in synchronized cell systems.
	Ing. Klára Hoyerová, Ph.D. hoyerova@ueb.cas.cz Regulation of the isopentenyl transferase expression in relation to enzyme targeting and cytokinin metabolism. Mechanisms of transport of auxins into Arabidopsis and tobacco cells.
	Prof. Ing. Miroslav Kamínek, Ph.D. kaminek@ueb.cas.cz Mechanisms of metabolic regulation of cytokinin levels in plant cells, their function in control of plant development and exploitation in enhancement of plant productivity.
	Ing. Kateřina Malínská, Ph.D. malinska@ueb.cas.cz Effect of phytotropins on cytoskeleton dynamics in tobacco BY2 cells. Yeast Saccharomyces cerevisiae as a tool to study mechanism of phytotropins action and auxin transport proteins. High resolution confocal microscopy on living cells, DNA cloning.
	Ing. Václav Motyka, Ph.D. motyka@ueb.cas.cz Regulation of the metabolic inactivation (degradation and N-glucosylation) of cytokinins in relation to control of plant growth and development. Metabolism and biological functions of cis-zeatin-type cytokinins in plants. Effects of biotic and abiotic stresses on the contents and metabolism of phytohormones.
	Ing. Karel Müller, Ph.D. muller@ueb.cas.cz Cloning of genes coding for tobacco auxin transporters, their expression and targeted silencing.
	RNDr. Jan Petrášek, Ph.D. petrasek@ueb.cas.cz, www The role of auxins and cytokinins in the regulation of cell division and elongation, hormon-autonomous growth of cell lines, the identification and visualisation of cellular structures playing role in the polar transport of auxin.
	Ing. Petr Skůpa, Ph.D. skupa@ueb.cas.cz Changes of phenotype of cell suspension in relation to the expression of transgenes affecting hormonal regulations in plant cells.
	RNDr. Radomíra Vaňková, Ph.D. vankova@ueb.cas.cz Regulation of the level of physiologically active cytokinins during the plant development by their reversible and irreversible glycosylation. The role of cytokinins and abscisic acid in plant response to abiotic stress and subsequent recovery.

Research assistants

	RNDr. Alena Gaudinová gaudinova@ueb.cas.cz Analytical methods for phytohormones; determination of level and activity of the enzymes of cytokinin biosynthesis and metabolism.
	Mgr. Markéta Pařezová parezova@ueb.cas.cz Transformation of tobacco and Arabidopsis suspension cultures.

Postdocs

	Ing. Milada Čovanová, Ph.D. covanova@ueb.cas.cz Role of Auxin Binding Protein 1 in the development of BY-2 tobacco cells and its involvement in the regulation of auxin level inside the cells.
	RNDr. Adriana Jelínková, Ph.D. cernaa@ueb.cas.cz Identification of cytoskeletal and associated proteins that are involved in dynamics od endomembrane transport, polar PIN targetting and thus on cell polarity establishment.
	Mgr. Petr Klíma, Ph.D. klima@ueb.cas.cz Mechanism of cytokinin transport across cell membranes, characterisation of carriers.
	RNDr. Martin Kubeš, Ph.D. kubes@ueb.cas.cz The role of ER-localized PIN5 and PILS transporters in regulation of homeostatic intracellular auxin levels.
	RNDr. Martina Laňková, Ph.D. lankova@ueb.cas.cz Mechanism of transport of plant hormone auxin into tobacco cells, characterisation of an auxin influx carrier and its inhibitors.

Postgraduate students

	Innu Kottanal Baby, M.Sc. baby@ueb.cas.cz Ph.D. dissertation theme: The role of cytoskeleton in the auxin-regulated plant morphogenesis. Faculty of Science, Charles University in Prague. Since 2012. Supervisor: J. Petrášek
	Mgr. Zdeněk Cit cit@ueb.cas.cz Ph.D. dissertation theme: Dynamics and function of plasma membrane carriers of auxin studied with advanced fluorescence microscopy techniques. Faculty of Science, Charles University in Prague. Since 2012. Supervisor: J. Petrášek, advisors: M. Hof a J. Humpolíčková (ÚFCH JH AVČR), E. Zažímalová
	Mgr. Matouš Glanc glanc@ueb.cas.cz Ph.D. dissertation theme: The role of endoplasmic reticulum in auxin signalling and metabolism. Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2014. Supervisor: J. Friml (IST, Klosterneuburg, Austria), advisor: E. Zažímalová
	Ing. Petr Hošek petr.hosek@fbmi.cvut.cz Ph.D. dissertation theme: Auxin flux mathematical modelling at the cellular level. FBMI ČVUT. Supervisor: M. Jiřina FBMI ČVUT, advisor: K. Hoyerová
	Ing. Jozef Lacek lacek@ueb.cas.cz Ph.D. dissertation theme: Protein ABP1 and its role in regulation of plant cell development. Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2013. Supervisor: E. Zažímalová, advisor: M. Čovanová
	Mgr. Sylva Přerostová prerostova@ueb.cas.cz Ph.D. dissertation theme: Characterization of the role of cytokinins and abscisic acid during abiotic stress response. Faculty of Science, Charles University in Prague. Since 2011. Supervisor: R. Vaňková
	Mgr. Roman Skokan letajici.kiwi@seznam.cz Ph.D. dissertation theme: Evolution of auxin homeostasis mechanisms. Faculty of Science, Charles University in Prague. Department of Experimental Plant Biology. Since 2014. Supervisor: J. Petrášek, advisor: K. Müller
	Ing. Eva Žižková zizkovae@ueb.cas.cz Ph.D. dissertation theme: Metabolic pathways regulating the levels of bioactive cytokinins in plants. Faculty of Science, Charles University in Prague. Since 2008. Supervisor: V. Motyka, advisor: K. Hoyerová

Diploma students

Technical assistants

	Mgr. Nikola Drážná drazna@ueb.cas.cz Isolation of plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
	Bc. Vojtěch Knirsch knirsch@ueb.cas.cz Cultivation of plant material, sampling and sample preparation, isolation of plant hormones, membrane integrity measurement.
	Marie Korecká korecka@ueb.cas.cz Isolation of the plant hormones auxins and cytokinins from plant tissues. Isolation of cytokininoxidase from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
	Karolína Müllerová mullerovak@ueb.cas.cz Preparation of cultivation media, cultivation of plant material.

Alumni

	Mgr. Jana Dobrá dobra@ueb.cas.cz Ph.D. dissertation theme: Role of cytokinins in abiotic stress. Faculty of Science, Charles University in Prague. Since 2007. Supervisor: R. Vaňková
	Mgr. Silvia Gajdošová, Ph.D. gajdosova@ueb.cas.cz Biological effects and metabolism of cis-zeatin type cytokinins in plants.
	RNDr. Marie Havlová, Ph.D. novakovam@ueb.cas.cz Regulation of cytokinin metabolism in plants, cell suspensions and chloroplasts.
	Mgr. Andrej Hurný, ->VIB, Ghent, Belgium andrej.hurny@gmail.com Diploma thesis theme: The role of transcriptional regulation of PIN proteins, membrane transporters of plant hormone auxin. Faculty of Science, Charles University in Prague. Supervisor: J. Petrášek, advisor: J. Libus
	Eva Kobzová kobzova@ueb.cas.cz Isolation of the plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).
	Mgr. Pavel Křeček, Ph.D. krecek@ueb.cas.cz Mechanisms of regulation of activity of auxin-efflux-carrier complex.
	RNDr. Jiří Libus, Ph.D. libus@ueb.cas.cz Transcription regulation of PIN genes.
	Mgr. Hana Lukšanová, Ph.D. luksanova@ueb.cas.cz Analysis of proteomic changes in plants under biotic and abiotic stress.
	Mgr. Marie Martincová martinc1@natur.cuni.cz Diploma thesis theme: The role of AGC protein kinases in the regulation of auxin transport. Faculty of Science, Charles University in Prague. Supervisor: J. Petrášek
	Mgr. Eva Miklánková miklankova@ueb.cas.cz Ph.D. dissertation theme: The study of gene expression during heat stress. Faculty of Science, Charles University in Prague. Since 2010. Supervisor: R. Vaňková
	Mgr. Daniela Seifertová, Ph.D. seifertova@ueb.cas.cz Study of the carriers of plant hormone auxin at the cellular level in Arabidopsis thaliana.
	Mgr. Sibu Simon, Ph.D., ->VIB, Ghent, Belgium simon@ueb.cas.cz Ph.D. dissertation theme: Mechanism of auxin transport – biochemical characterisation of auxin efflux from cells. Faculty of Science, Charles University in Prague. Supervisor: E. Zažímalová, advisor: J. Petrášek
	Ing. Jana Žabová zabovaj@ueb.cas.cz Isolation of the plant hormones auxins and cytokinins from plant tissues. Cultivation of plant material (in vitro cultures, greenhouse).